Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE/cglE   Type   Machinery gene
Locus tag   E0F31_RS10055 Genome accession   NZ_LR216064
Coordinates   1863253..1863555 (-) Length   100 a.a.
NCBI ID   WP_257641679.1    Uniprot ID   A0AA95D7F2
Organism   Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1858253..1868555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F31_RS10025 (SAMEA3714520_01889) rnpA 1858939..1859310 (-) 372 WP_000739248.1 ribonuclease P protein component -
  E0F31_RS12135 - 1859327..1859458 (-) 132 WP_000768902.1 hypothetical protein -
  E0F31_RS10030 (SAMEA3714520_01890) - 1859459..1860649 (-) 1191 WP_050085692.1 acetate kinase -
  E0F31_RS10035 (SAMEA3714520_01891) comYH 1860700..1861653 (-) 954 WP_000345083.1 class I SAM-dependent methyltransferase Machinery gene
  E0F31_RS10040 (SAMEA3714520_01892) - 1861714..1862301 (-) 588 WP_000679775.1 class I SAM-dependent methyltransferase -
  E0F31_RS10045 (SAMEA3714520_01893) comGG/cglG 1862438..1862851 (-) 414 WP_000265617.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F31_RS10050 (SAMEA3714520_01894) comGF/cglF 1862829..1863290 (-) 462 WP_000250546.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F31_RS10055 (SAMEA3714520_01895) comGE/cglE 1863253..1863555 (-) 303 WP_257641679.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F31_RS10060 (SAMEA3714520_01896) comGD/cglD 1863518..1863922 (-) 405 WP_000588016.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F31_RS10065 (SAMEA3714520_01897) comGC/cglC 1863915..1864241 (-) 327 WP_000738632.1 comG operon protein ComGC Machinery gene
  E0F31_RS10070 (SAMEA3714520_01898) comGB/cglB 1864243..1865259 (-) 1017 WP_077141332.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F31_RS10075 (SAMEA3714520_01899) comGA/cglA/cilD 1865207..1866148 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F31_RS10080 (SAMEA3714520_01900) - 1866224..1866589 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F31_RS10085 (SAMEA3714520_01901) - 1866740..1867798 (-) 1059 WP_000649470.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 100 a.a.        Molecular weight: 11149.14 Da        Isoelectric Point: 9.7273

>NTDB_id=1126297 E0F31_RS10055 WP_257641679.1 1863253..1863555(-) (comGE/cglE) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVAKMALQTGQNQININGVEIQVFS
SEKGLEVYHGSEQLLAIKEP

Nucleotide


Download         Length: 303 bp        

>NTDB_id=1126297 E0F31_RS10055 WP_257641679.1 1863253..1863555(-) (comGE/cglE) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
ATGGAAAAATTAAACGCATTAAGGAAACAAAAAATTAGGGCAGTGATTTTACTGGAAGCAGTAGTCGCTCTAGCTATCTT
TGCCAGCATTGCGACCCTCCTTTTGGGACAAATTCAAAAAAATAGGCAAGAGGAAGCAAAAATCTTGCAAAAGGAAGAAG
TCTTGAGGGTAGCTAAGATGGCCTTGCAGACGGGGCAAAATCAGATAAACATCAACGGAGTTGAGATTCAGGTATTTTCT
AGTGAAAAAGGATTGGAGGTCTACCATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAA

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(16-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE/cglE Streptococcus mitis NCTC 12261

98

100

0.98
  comGE/cglE Streptococcus pneumoniae Rx1

98

100

0.98
  comGE/cglE Streptococcus pneumoniae D39

98

100

0.98
  comGE/cglE Streptococcus pneumoniae R6

98

100

0.98
  comGE/cglE Streptococcus pneumoniae TIGR4

98

100

0.98
  comGE/cglE Streptococcus mitis SK321

97

100

0.97
  comYE Streptococcus mutans UA140

43.333

90

0.39
  comYE Streptococcus mutans UA159

43.333

90

0.39

Multiple sequence alignment