Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   EL276_RS08500 Genome accession   NZ_LR134516
Coordinates   1849822..1850277 (+) Length   151 a.a.
NCBI ID   WP_107928640.1    Uniprot ID   A0A3S4Y905
Organism   Neisseria animaloris strain NCTC12227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1844822..1855277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL276_RS08475 (NCTC12227_01726) - 1845578..1846372 (-) 795 WP_126304991.1 3'-5' exonuclease -
  EL276_RS08480 (NCTC12227_01727) - 1846597..1847373 (+) 777 WP_126304992.1 DsbC family protein -
  EL276_RS08485 (NCTC12227_01728) - 1847517..1848398 (+) 882 WP_126304993.1 prephenate dehydrogenase -
  EL276_RS08490 (NCTC12227_01729) - 1848477..1848965 (+) 489 WP_126304994.1 disulfide bond formation protein B -
  EL276_RS08495 - 1848970..1849179 (-) 210 WP_126304995.1 hypothetical protein -
  EL276_RS08500 (NCTC12227_01730) pilE 1849822..1850277 (+) 456 WP_107928640.1 pilin Machinery gene
  EL276_RS10915 (NCTC12227_01731) - 1850363..1850809 (+) 447 WP_107928639.1 pilin -
  EL276_RS08510 (NCTC12227_01732) - 1850940..1851356 (-) 417 WP_085390946.1 hypothetical protein -
  EL276_RS08515 (NCTC12227_01733) - 1852200..1853723 (+) 1524 WP_126304996.1 fumarate hydratase -
  EL276_RS08520 (NCTC12227_01734) - 1853931..1854641 (+) 711 WP_107879853.1 SIMPL domain-containing protein -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15582.88 Da        Isoelectric Point: 7.7688

>NTDB_id=1123737 EL276_RS08500 WP_107928640.1 1849822..1850277(+) (pilE) [Neisseria animaloris strain NCTC12227]
MKAIQKGFTLIELMIVVAIIGILASIALPAYQDYTARAQVSEALTLADGQKTAVTEYYADKGELPENNDKAGIAPATDIK
GKYVTSVTVSKGVITALLNSTGLADGIKGASVILTPTTNNGSVSWSCSTAKTGGSGTDPLEAKYIPAACRK

Nucleotide


Download         Length: 456 bp        

>NTDB_id=1123737 EL276_RS08500 WP_107928640.1 1849822..1850277(+) (pilE) [Neisseria animaloris strain NCTC12227]
ATGAAAGCTATTCAAAAAGGTTTTACCCTGATCGAGTTGATGATCGTTGTAGCGATTATCGGTATTTTGGCATCAATCGC
ATTGCCTGCTTACCAAGACTATACCGCTCGCGCCCAAGTATCTGAAGCACTAACCTTGGCCGATGGTCAAAAAACGGCTG
TTACTGAGTACTATGCAGATAAAGGTGAATTACCTGAAAACAATGATAAAGCAGGTATTGCTCCCGCCACAGACATTAAA
GGTAAATATGTAACATCTGTTACAGTGTCAAAAGGTGTAATTACAGCACTGCTAAATTCTACAGGTTTGGCCGACGGTAT
CAAAGGAGCTTCTGTAATATTAACACCTACAACTAATAATGGCTCGGTATCTTGGTCATGTTCAACTGCGAAAACTGGTG
GTTCTGGTACCGACCCCCTTGAGGCTAAATATATTCCTGCAGCTTGCCGCAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4Y905

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

55

100

0.583
  pilE Neisseria gonorrhoeae strain FA1090

52.532

100

0.55
  pilA2 Legionella pneumophila strain ERS1305867

51.007

98.675

0.503
  pilA2 Legionella pneumophila str. Paris

50.336

98.675

0.497
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.83

100

0.483
  pilA Ralstonia pseudosolanacearum GMI1000

39.157

100

0.43
  pilA/pilA1 Eikenella corrodens VA1

40.252

100

0.424
  comP Acinetobacter baylyi ADP1

40.789

100

0.411
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.474

100

0.397
  pilA Pseudomonas aeruginosa PAK

36.42

100

0.391
  pilA/pilAII Pseudomonas stutzeri DSM 10701

35.948

100

0.364

Multiple sequence alignment