Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL143_RS08145 Genome accession   NZ_LR134313
Coordinates   1733267..1734406 (-) Length   379 a.a.
NCBI ID   WP_085415913.1    Uniprot ID   A0A1X3CYV8
Organism   Neisseria canis strain NCTC10296     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1728267..1739406
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL143_RS08120 (NCTC10296_01633) - 1729247..1730503 (-) 1257 WP_085415916.1 murein hydrolase activator EnvC -
  EL143_RS08125 (NCTC10296_01634) - 1730642..1731190 (+) 549 WP_085416001.1 YqgE/AlgH family protein -
  EL143_RS08130 (NCTC10296_01635) ruvX 1731192..1731665 (+) 474 WP_085416000.1 Holliday junction resolvase RuvX -
  EL143_RS08135 (NCTC10296_01636) - 1731755..1732168 (+) 414 WP_085415915.1 YidB family protein -
  EL143_RS08140 (NCTC10296_01637) argB 1732292..1733182 (+) 891 WP_085415914.1 acetylglutamate kinase -
  EL143_RS08145 (NCTC10296_01638) pilU 1733267..1734406 (-) 1140 WP_085415913.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL143_RS08150 (NCTC10296_01639) - 1734724..1735425 (+) 702 WP_085415912.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  EL143_RS08155 (NCTC10296_01640) - 1735444..1735821 (+) 378 WP_085415911.1 hypothetical protein -
  EL143_RS08160 (NCTC10296_01641) cysT 1736118..1736945 (+) 828 WP_085415999.1 sulfate ABC transporter permease subunit CysT -
  EL143_RS08165 (NCTC10296_01642) cysW 1736968..1737819 (+) 852 WP_085415910.1 sulfate ABC transporter permease subunit CysW -
  EL143_RS08170 (NCTC10296_01643) - 1737865..1738947 (+) 1083 WP_085415909.1 sulfate/molybdate ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42856.89 Da        Isoelectric Point: 6.1323

>NTDB_id=1121100 EL143_RS08145 WP_085415913.1 1733267..1734406(-) (pilU) [Neisseria canis strain NCTC10296]
MDNTLHDSIDTKDELYQWLRYMVQYKASDLFITTGFPPAVKLDGKITRLNDQPLTPERCIEIAASVMNPKQLEEFTTTSE
CNFAIQLPGTARFRVNAMVQRGATALVFRQISSEIPSFEQLNLPPVLQQVVMRKRGLVIFVGGTGSGKSTSLASLIDYRN
QHGNDHIITIEDPIEFVHQHKNCIITQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMEYAMAFAETGHLCMATL
HANSSNQALDRIINFFPEERRNQLLTDLSLNLQAFISQRLIPLEHGRGRIAAVEVLLNSPLISDLIHRGEIHHIKEIMKK
STDIGMQTFDQALYNLYEQGSISFQEAIKNADSANDLRLDIQLRSRRGRSQGVDLEIIG

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1121100 EL143_RS08145 WP_085415913.1 1733267..1734406(-) (pilU) [Neisseria canis strain NCTC10296]
ATGGATAACACATTACACGATTCAATTGACACAAAAGACGAGCTTTATCAATGGCTCCGATATATGGTGCAATACAAAGC
GTCCGATTTATTCATCACCACCGGCTTCCCCCCCGCAGTCAAGCTCGACGGTAAAATTACCCGCTTAAACGACCAGCCGC
TCACACCGGAGCGCTGTATCGAAATTGCTGCTTCGGTAATGAATCCCAAACAGCTTGAAGAATTTACAACCACCAGCGAA
TGCAATTTCGCCATCCAGCTTCCCGGCACCGCCCGTTTCCGCGTCAACGCAATGGTTCAACGCGGGGCAACGGCTTTGGT
GTTCCGCCAAATCAGCAGCGAAATCCCCTCTTTCGAGCAGCTGAACCTGCCGCCCGTTTTGCAGCAAGTGGTAATGCGCA
AACGCGGCCTGGTGATTTTCGTGGGCGGAACCGGCTCCGGCAAATCCACTTCGCTGGCATCGCTGATCGACTACCGCAAC
CAACACGGCAACGACCACATCATTACCATCGAAGATCCTATCGAATTCGTACACCAGCATAAAAACTGCATCATCACGCA
GCGCGAAGTGGGCGTGGACACCGAAAACTGGTTTGCCGCCCTGAAAAACACATTGCGCCAAGCGCCTGATGTGATTTTGA
TCGGCGAGATTCGCGACCGCGAAACCATGGAATATGCCATGGCGTTTGCCGAAACAGGCCACTTATGTATGGCCACTTTG
CACGCCAACAGCTCCAACCAAGCGCTCGACCGCATTATCAACTTCTTCCCCGAAGAGCGCCGCAACCAGCTTTTGACCGA
TTTGTCGTTGAACCTGCAAGCCTTTATTTCGCAGCGCCTCATCCCGCTGGAACACGGCAGGGGTCGGATTGCGGCTGTGG
AAGTGTTACTGAATTCACCGCTCATCTCCGATTTGATTCACCGCGGCGAAATCCACCACATCAAAGAGATTATGAAAAAA
TCCACCGATATCGGCATGCAAACTTTCGACCAGGCTTTATACAACCTGTACGAACAAGGCTCTATCAGTTTTCAGGAAGC
CATCAAAAACGCCGATTCGGCCAACGACCTGCGCCTCGACATCCAGTTGCGCAGCCGCCGCGGCCGCAGCCAGGGGGTTG
ATTTGGAAATTATCGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X3CYV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.919

97.625

0.575
  pilU Acinetobacter baylyi ADP1

57.637

91.557

0.528
  pilU Vibrio cholerae strain A1552

55.432

94.723

0.525
  pilT Legionella pneumophila strain Lp02

43.413

88.127

0.383
  pilT Legionella pneumophila strain ERS1305867

43.413

88.127

0.383
  pilT Acinetobacter baylyi ADP1

41.471

89.71

0.372
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.716

89.182

0.372
  pilT Neisseria meningitidis 8013

40

91.029

0.364
  pilT Pseudomonas aeruginosa PAK

40.828

89.182

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361

Multiple sequence alignment