Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   EL115_RS08400 Genome accession   NZ_LR134307
Coordinates   1674248..1675189 (-) Length   313 a.a.
NCBI ID   WP_126441774.1    Uniprot ID   -
Organism   Streptococcus milleri strain NCTC10708     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1669248..1680189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL115_RS08360 (NCTC10708_01657) - 1669305..1670504 (-) 1200 WP_126441770.1 acetate kinase -
  EL115_RS08365 (NCTC10708_01658) comYH 1670551..1671504 (-) 954 WP_115263521.1 class I SAM-dependent methyltransferase Machinery gene
  EL115_RS08370 (NCTC10708_01659) comGG 1671589..1671915 (-) 327 WP_003070783.1 competence type IV pilus minor pilin ComGG -
  EL115_RS08375 (NCTC10708_01660) comGF/cglF 1671896..1672333 (-) 438 WP_126441771.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL115_RS08380 (NCTC10708_01661) comGE/cglE 1672317..1672610 (-) 294 WP_126441772.1 competence type IV pilus minor pilin ComGE Machinery gene
  EL115_RS08385 (NCTC10708_01662) comYD 1672582..1672980 (-) 399 WP_006269995.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL115_RS08390 (NCTC10708_01663) comYC 1672970..1673287 (-) 318 WP_006269985.1 competence type IV pilus major pilin ComGC Machinery gene
  EL115_RS08395 (NCTC10708_01664) comYB 1673284..1674306 (-) 1023 WP_126441773.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL115_RS08400 (NCTC10708_01665) comYA 1674248..1675189 (-) 942 WP_126441774.1 competence type IV pilus ATPase ComGA Machinery gene
  EL115_RS08405 (NCTC10708_01666) - 1675259..1675624 (-) 366 WP_126441775.1 DUF1033 family protein -
  EL115_RS08410 (NCTC10708_01667) glnA 1675812..1677158 (-) 1347 WP_006269977.1 type I glutamate--ammonia ligase -
  EL115_RS08415 (NCTC10708_01668) - 1677197..1677556 (-) 360 WP_126441776.1 MerR family transcriptional regulator -
  EL115_RS08420 (NCTC10708_01669) - 1677633..1678163 (-) 531 WP_006269975.1 FUSC family protein -
  EL115_RS08425 - 1678303..1678587 (-) 285 WP_006269982.1 2'-5' RNA ligase family protein -
  EL115_RS08430 (NCTC10708_01670) - 1678590..1678796 (-) 207 WP_006268044.1 hypothetical protein -
  EL115_RS08435 (NCTC10708_01671) - 1678863..1680059 (-) 1197 WP_039677610.1 phosphoglycerate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 36001.16 Da        Isoelectric Point: 6.3145

>NTDB_id=1120917 EL115_RS08400 WP_126441774.1 1674248..1675189(-) (comYA) [Streptococcus milleri strain NCTC10708]
MVQNIAQAIICQAKEERAQDIYFVPKENVYELYMRIGDERRFIRNYEFEELAAVISHFKFTAGMNVGEKRRSQLGSCDYQ
YSDRKTSLRLSTVGDYRGYESLVIRLLHDEEAELKFWFAQIPELKERFKQRGLYLFSGPVGSGKTTLMHQLAQERFANQQ
IMSIEDPVEIKQAGMLQLQLNEAIGLTYESLIKLSLRHRPDLLIIGEIRDAETARAVVRASLTGVTVFSTIHAKSVRGVY
ERLLELGVTEAELKIVLQGICYQRLIAKGGIVDFVSERYQEHEPSDWNKQIDQLYAAGHITFEQAETEKIIHS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1120917 EL115_RS08400 WP_126441774.1 1674248..1675189(-) (comYA) [Streptococcus milleri strain NCTC10708]
ATGGTTCAAAACATTGCACAGGCTATTATTTGTCAGGCTAAGGAAGAACGAGCACAGGACATTTATTTTGTTCCGAAGGA
AAATGTTTACGAGTTGTATATGCGAATTGGAGATGAACGCCGTTTTATACGCAATTATGAATTTGAAGAGCTAGCTGCTG
TAATCAGTCATTTCAAGTTTACTGCTGGGATGAATGTGGGAGAAAAACGTCGCAGTCAGCTGGGCTCTTGCGATTATCAA
TATAGTGACAGGAAAACGTCGCTACGGTTGTCAACAGTAGGGGATTACCGTGGATATGAAAGCCTGGTGATTCGTCTATT
GCATGATGAAGAAGCAGAGCTGAAGTTTTGGTTTGCTCAAATTCCGGAGTTGAAAGAACGTTTCAAACAGCGAGGGCTTT
ACCTTTTTTCAGGACCGGTAGGAAGTGGTAAAACTACTCTCATGCACCAGTTGGCTCAAGAGCGTTTTGCCAATCAGCAA
ATCATGTCAATTGAAGATCCGGTTGAAATCAAACAAGCAGGGATGTTACAGCTGCAGCTGAACGAAGCGATTGGTTTAAC
TTATGAAAGTTTGATAAAACTTTCTCTTCGGCATCGACCGGATTTATTGATTATCGGAGAGATTCGTGATGCGGAGACAG
CACGGGCAGTTGTTCGAGCTAGCTTAACGGGAGTGACTGTTTTTTCAACTATTCATGCTAAAAGCGTACGAGGTGTCTAT
GAACGGTTGCTGGAGTTGGGAGTGACAGAAGCAGAGCTGAAAATTGTTTTGCAAGGAATTTGCTACCAACGGTTAATTGC
GAAAGGAGGAATAGTGGATTTTGTCAGTGAAAGGTATCAAGAGCATGAGCCAAGTGACTGGAATAAGCAGATTGATCAGC
TTTATGCAGCAGGACATATCACTTTTGAGCAGGCAGAAACGGAAAAAATTATCCATAGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus gordonii str. Challis substr. CH1

82.903

99.042

0.821
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

75.719

100

0.757
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

75.399

100

0.754
  comGA/cglA/cilD Streptococcus pneumoniae D39

75.399

100

0.754
  comGA/cglA/cilD Streptococcus pneumoniae R6

75.399

100

0.754
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

75.399

100

0.754
  comYA Streptococcus mutans UA159

64.63

99.361

0.642
  comYA Streptococcus mutans UA140

64.63

99.361

0.642
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.258

99.042

0.617
  comGA Lactococcus lactis subsp. cremoris KW2

54.662

99.361

0.543
  comGA Latilactobacillus sakei subsp. sakei 23K

42.403

90.415

0.383

Multiple sequence alignment