Detailed information NTG1120912

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: EL115_RS08375
• Genome accession: NZ_LR134307
• Coordinates: 1671896..1672333 (-)
• Length: 145 a.a.
• NCBI ID: WP_126441771.1
• Uniprot ID: -
• Organism: Streptococcus milleri strain NCTC10708
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1666896..1677333

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL115_RS08355 (NCTC10708_01656)	glmS	1667100..1668914 (-)	1815	WP_126441769.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
EL115_RS08360 (NCTC10708_01657)	-	1669305..1670504 (-)	1200	WP_126441770.1	acetate kinase	-
EL115_RS08365 (NCTC10708_01658)	comYH	1670551..1671504 (-)	954	WP_115263521.1	class I SAM-dependent methyltransferase	Machinery gene
EL115_RS08370 (NCTC10708_01659)	comGG	1671589..1671915 (-)	327	WP_003070783.1	competence type IV pilus minor pilin ComGG	-
EL115_RS08375 (NCTC10708_01660)	comGF/cglF	1671896..1672333 (-)	438	WP_126441771.1	competence type IV pilus minor pilin ComGF	Machinery gene
EL115_RS08380 (NCTC10708_01661)	comGE/cglE	1672317..1672610 (-)	294	WP_126441772.1	competence type IV pilus minor pilin ComGE	Machinery gene
EL115_RS08385 (NCTC10708_01662)	comYD	1672582..1672980 (-)	399	WP_006269995.1	competence type IV pilus minor pilin ComGD	Machinery gene
EL115_RS08390 (NCTC10708_01663)	comYC	1672970..1673287 (-)	318	WP_006269985.1	competence type IV pilus major pilin ComGC	Machinery gene
EL115_RS08395 (NCTC10708_01664)	comYB	1673284..1674306 (-)	1023	WP_126441773.1	competence type IV pilus assembly protein ComGB	Machinery gene
EL115_RS08400 (NCTC10708_01665)	comYA	1674248..1675189 (-)	942	WP_126441774.1	competence type IV pilus ATPase ComGA	Machinery gene
EL115_RS08405 (NCTC10708_01666)	-	1675259..1675624 (-)	366	WP_126441775.1	DUF1033 family protein	-
EL115_RS08410 (NCTC10708_01667)	glnA	1675812..1677158 (-)	1347	WP_006269977.1	type I glutamate--ammonia ligase	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16895.60 Da
• Isoelectric Point: 10.6422

>NTDB_id=1120912 EL115_RS08375 WP_126441771.1 1671896..1672333(-) (comGF/cglF) [Streptococcus milleri strain NCTC10708]
MFKANKVKAFTLLEALVALFVISGSVLLFQSMTRLLASEVRARQHSEQREWLLFTHQLEAELIRSSFEKVENNRLYMKQD
GKTIAFGKSSGQDFRKTNANGKGYQPMIYNVKKAEISQKNELIHIRMTFKRGLEREFVYRVETKS

Nucleotide

• Length: 438 bp

>NTDB_id=1120912 EL115_RS08375 WP_126441771.1 1671896..1672333(-) (comGF/cglF) [Streptococcus milleri strain NCTC10708]
GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACACTTTTAGAGGCTCTGGTTGCCCTCTTTGTAATCAGCGGGAGTGTGCT
ATTATTTCAATCGATGACGCGTCTTCTGGCTTCGGAGGTCCGAGCTCGACAACACAGTGAACAACGAGAATGGCTTTTGT
TTACCCATCAATTAGAAGCGGAATTGATTCGTTCTTCATTTGAAAAAGTGGAGAATAATCGTTTATACATGAAGCAGGAC
GGTAAGACGATTGCATTTGGAAAGTCAAGTGGACAAGATTTCAGAAAAACCAATGCAAATGGGAAAGGCTATCAGCCTAT
GATTTATAATGTGAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTCATATCAGAATGACATTTAAGCGAGGATTGG
AAAGGGAGTTCGTCTATCGTGTGGAAACAAAGAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae TIGR4	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae Rx1	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae D39	66.429	96.552	0.641
comGF/cglF	Streptococcus mitis NCTC 12261	65.714	96.552	0.634
comGF/cglF	Streptococcus mitis SK321	65.714	96.552	0.634
comYF	Streptococcus mutans UA159	43.972	97.241	0.428
comYF	Streptococcus mutans UA140	43.972	97.241	0.428
comGF	Lactococcus lactis subsp. cremoris KW2	42.963	93.103	0.4