Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   EL084_RS00145 Genome accession   NZ_LR134292
Coordinates   24538..25479 (+) Length   313 a.a.
NCBI ID   WP_126439866.1    Uniprot ID   -
Organism   Streptococcus equinus strain NCTC10389     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 19538..30479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL084_RS00135 (NCTC10389_00030) rpoC 20296..23934 (+) 3639 WP_126439864.1 DNA-directed RNA polymerase subunit beta' -
  EL084_RS00140 (NCTC10389_00031) - 24101..24469 (+) 369 WP_126439865.1 DUF1033 family protein -
  EL084_RS00145 (NCTC10389_00032) comYA 24538..25479 (+) 942 WP_126439866.1 competence type IV pilus ATPase ComGA Machinery gene
  EL084_RS00150 (NCTC10389_00033) comYB 25403..26446 (+) 1044 WP_164557721.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL084_RS00155 (NCTC10389_00034) comYC 26446..26748 (+) 303 WP_003067906.1 competence type IV pilus major pilin ComGC Machinery gene
  EL084_RS00160 (NCTC10389_00035) comGD 26723..27163 (+) 441 WP_003067905.1 competence type IV pilus minor pilin ComGD -
  EL084_RS00165 (NCTC10389_00036) comGE 27117..27410 (+) 294 WP_003067904.1 competence type IV pilus minor pilin ComGE -
  EL084_RS00170 (NCTC10389_00037) comYF 27394..27831 (+) 438 WP_003067903.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL084_RS00175 (NCTC10389_00038) comGG 27842..28153 (+) 312 WP_268921724.1 competence type IV pilus minor pilin ComGG -
  EL084_RS00180 (NCTC10389_00039) comYH 28207..29163 (+) 957 WP_115254812.1 class I SAM-dependent methyltransferase Machinery gene
  EL084_RS00185 (NCTC10389_00040) - 29217..30416 (+) 1200 WP_126439867.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35719.88 Da        Isoelectric Point: 6.3684

>NTDB_id=1120412 EL084_RS00145 WP_126439866.1 24538..25479(+) (comYA) [Streptococcus equinus strain NCTC10389]
MIQEKAKQLIRDAVGKNAQDIYIIPKAENYEIYQRIGEKRQFIQEVATDEMTSLISHFKFVAGMNVGEKRRSQLGSCDYA
FADDEEISLRLSSVGDYRGRESLVIRLLYSGRHDLQYWFDGMRNILEAIDSRGLYLFSGPVGSGKTTLMYQLVREKFPGK
QVITIEDPVEIKQDNMLQLQLNDEIGMTYDNLIKLSLRHRPDILIIGEIRDSETARAVIRASLTGVLVFSTIHAKSIPGV
YARLLELGVSRQELENSLRAIVYQRLIGGGGVVDFAKRDFEQYPPRRWNEQIDSLARDGHISFEEAKIEKIAL

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1120412 EL084_RS00145 WP_126439866.1 24538..25479(+) (comYA) [Streptococcus equinus strain NCTC10389]
ATGATTCAGGAAAAAGCAAAGCAGCTTATTAGAGATGCAGTTGGAAAGAATGCTCAGGATATCTATATTATTCCAAAGGC
AGAAAACTATGAGATTTATCAACGAATTGGTGAGAAACGCCAGTTTATTCAAGAAGTTGCTACTGATGAAATGACAAGCT
TGATTAGTCACTTTAAATTTGTAGCTGGGATGAATGTTGGTGAAAAGCGTCGTAGTCAGCTTGGTTCTTGTGACTATGCT
TTTGCAGATGATGAGGAGATTTCACTGCGCTTATCTAGTGTGGGTGATTATCGTGGGCGTGAAAGTCTGGTCATTAGGCT
CCTTTATTCAGGTCGTCATGATTTGCAGTATTGGTTTGATGGAATGAGAAATATTTTAGAGGCTATTGACAGCAGGGGAC
TTTACCTTTTTTCCGGGCCGGTAGGGAGTGGAAAAACCACTCTCATGTACCAATTAGTTCGTGAAAAATTTCCTGGAAAA
CAGGTGATTACGATTGAAGACCCTGTTGAGATAAAACAAGACAATATGTTGCAATTGCAGTTAAATGATGAAATCGGGAT
GACTTATGATAATCTTATCAAGCTCTCGCTTCGTCATAGACCTGATATTTTGATTATTGGTGAGATTCGTGATAGTGAGA
CAGCGCGTGCGGTTATTAGAGCTAGTCTGACAGGAGTCCTTGTTTTTTCAACCATTCATGCGAAAAGTATTCCAGGTGTT
TATGCTAGGTTATTAGAATTGGGTGTTAGTAGGCAGGAACTTGAAAATAGCTTGCGTGCTATTGTTTATCAACGATTAAT
TGGTGGTGGAGGTGTAGTTGATTTTGCCAAAAGAGATTTTGAGCAGTATCCCCCAAGAAGGTGGAATGAGCAAATTGATA
GCCTTGCTCGAGACGGACATATCAGTTTTGAAGAGGCAAAAATCGAAAAAATTGCCCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

71.061

99.361

0.706
  comYA Streptococcus mutans UA140

70.74

99.361

0.703
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

68.387

99.042

0.677
  comYA Streptococcus gordonii str. Challis substr. CH1

65.273

99.361

0.649
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.103

99.681

0.639
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.103

99.681

0.639
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.103

99.681

0.639
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.103

99.681

0.639
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

63.782

99.681

0.636
  comGA Lactococcus lactis subsp. cremoris KW2

54.633

100

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.701

87.54

0.374

Multiple sequence alignment