Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   EL084_RS00150 Genome accession   NZ_LR134292
Coordinates   25403..26446 (+) Length   347 a.a.
NCBI ID   WP_164557721.1    Uniprot ID   -
Organism   Streptococcus equinus strain NCTC10389     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 20403..31446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL084_RS00140 (NCTC10389_00031) - 24101..24469 (+) 369 WP_126439865.1 DUF1033 family protein -
  EL084_RS00145 (NCTC10389_00032) comYA 24538..25479 (+) 942 WP_126439866.1 competence type IV pilus ATPase ComGA Machinery gene
  EL084_RS00150 (NCTC10389_00033) comYB 25403..26446 (+) 1044 WP_164557721.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL084_RS00155 (NCTC10389_00034) comYC 26446..26748 (+) 303 WP_003067906.1 competence type IV pilus major pilin ComGC Machinery gene
  EL084_RS00160 (NCTC10389_00035) comGD 26723..27163 (+) 441 WP_003067905.1 competence type IV pilus minor pilin ComGD -
  EL084_RS00165 (NCTC10389_00036) comGE 27117..27410 (+) 294 WP_003067904.1 competence type IV pilus minor pilin ComGE -
  EL084_RS00170 (NCTC10389_00037) comYF 27394..27831 (+) 438 WP_003067903.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL084_RS00175 (NCTC10389_00038) comGG 27842..28153 (+) 312 WP_268921724.1 competence type IV pilus minor pilin ComGG -
  EL084_RS00180 (NCTC10389_00039) comYH 28207..29163 (+) 957 WP_115254812.1 class I SAM-dependent methyltransferase Machinery gene
  EL084_RS00185 (NCTC10389_00040) - 29217..30416 (+) 1200 WP_126439867.1 acetate kinase -
  EL084_RS00190 (NCTC10389_00041) - 30574..30771 (+) 198 WP_126439868.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39727.14 Da        Isoelectric Point: 9.8731

>NTDB_id=1120413 EL084_RS00150 WP_164557721.1 25403..26446(+) (comYB) [Streptococcus equinus strain NCTC10389]
MSKLIALLETDISVLKRQKSKKLPFKKQQKVIQLFNNLFNSGFTLTEIVSFLRRSQLLQESYVNSMQESLLSGATLADMM
ARLGFSDTIVTQIALADVHGNCQQSLVKINRYLASMSVVRKKLIEVATYPLILLSFLILIMLGLKNYLLPQLESQNFATQ
IIAHFPIIFLGSFVLLGLAVVLGFFYARRLSPIYLFSQISRLPIIGRFIRLYLTAYYAREWGNLIGQGIDLMEIVALMQH
QKSRLFQEIGKDMQQALLSGQSFHQKVLDYPFFLRELSLMIEYGEVKSKLGRELDIYAEETWQTFFSQLTQATQLIQPLV
FVFVALIIVLIYVAMLLPMYQNMGGNF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1120413 EL084_RS00150 WP_164557721.1 25403..26446(+) (comYB) [Streptococcus equinus strain NCTC10389]
ATGAGCAAATTGATAGCCTTGCTCGAGACGGACATATCAGTTTTGAAGAGGCAAAAATCGAAAAAATTGCCCTTTAAAAA
ACAACAGAAGGTCATTCAATTATTTAATAATTTGTTTAACAGTGGCTTTACTTTAACTGAGATAGTGTCTTTTTTGAGAC
GGAGTCAGTTATTGCAGGAATCATATGTGAATAGTATGCAGGAGTCCTTGCTGAGTGGCGCAACTTTAGCAGATATGATG
GCAAGGTTAGGCTTTTCAGATACGATTGTGACTCAGATTGCTTTGGCTGATGTTCATGGAAATTGTCAGCAAAGTCTTGT
AAAAATCAATCGTTACTTGGCTAGCATGTCTGTTGTGCGAAAGAAGTTGATTGAAGTAGCGACCTATCCTTTGATTTTAT
TAAGTTTTTTGATTTTGATAATGCTTGGCTTAAAAAATTATTTATTGCCGCAACTTGAAAGTCAAAATTTTGCTACTCAG
ATTATTGCCCATTTTCCAATAATTTTTTTGGGGAGCTTTGTGCTTTTAGGACTAGCGGTTGTTTTGGGATTTTTCTATGC
TAGGCGTTTATCACCAATCTACCTTTTTAGCCAGATTAGTCGCTTGCCGATAATAGGGAGATTCATTCGTCTTTATCTAA
CAGCTTATTATGCGCGTGAGTGGGGAAATCTTATTGGGCAGGGGATTGATTTGATGGAAATTGTAGCATTAATGCAACAT
CAAAAATCTCGTCTTTTTCAAGAGATTGGTAAGGATATGCAGCAAGCTCTTTTGTCTGGTCAATCATTTCATCAAAAAGT
TCTAGATTATCCCTTCTTTTTGAGGGAACTGAGCCTGATGATTGAGTACGGAGAAGTTAAGTCAAAACTTGGTCGGGAGC
TTGATATCTATGCTGAGGAAACATGGCAGACTTTCTTCAGCCAATTAACTCAGGCAACACAGCTGATTCAACCGCTAGTT
TTTGTCTTTGTTGCTTTAATTATTGTTTTAATCTACGTGGCAATGCTTTTGCCAATGTATCAAAATATGGGAGGAAATTT
TTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

63.158

98.559

0.622
  comYB Streptococcus mutans UA159

63.158

98.559

0.622
  comYB Streptococcus gordonii str. Challis substr. CH1

58.601

98.847

0.579
  comGB/cglB Streptococcus mitis NCTC 12261

58.16

97.118

0.565
  comGB/cglB Streptococcus mitis SK321

57.567

97.118

0.559
  comGB/cglB Streptococcus pneumoniae Rx1

57.27

97.118

0.556
  comGB/cglB Streptococcus pneumoniae D39

57.27

97.118

0.556
  comGB/cglB Streptococcus pneumoniae R6

57.27

97.118

0.556
  comGB/cglB Streptococcus pneumoniae TIGR4

57.27

97.118

0.556
  comGB Lactococcus lactis subsp. cremoris KW2

50.296

97.406

0.49

Multiple sequence alignment