Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssb   Type   Machinery gene
Locus tag   EW021_RS05345 Genome accession   NZ_LR130236
Coordinates   1028704..1029129 (-) Length   141 a.a.
NCBI ID   WP_011285575.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 5448 isolate 5448     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 993445..1039755 1028704..1029129 within 0


Gene organization within MGE regions


Location: 993445..1039755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW021_RS05115 pfkA 993445..994458 (-) 1014 WP_010922375.1 6-phosphofructokinase -
  EW021_RS05120 - 994538..997648 (-) 3111 WP_010922376.1 DNA polymerase III subunit alpha -
  EW021_RS05125 - 997833..998204 (+) 372 WP_010922377.1 GntR family transcriptional regulator -
  EW021_RS05130 - 998204..998902 (+) 699 WP_002984437.1 ABC transporter ATP-binding protein -
  EW021_RS05135 - 998912..999697 (+) 786 WP_010922378.1 hypothetical protein -
  EW021_RS05140 - 999824..1000438 (-) 615 WP_011285558.1 TVP38/TMEM64 family protein -
  EW021_RS05150 prx 1001029..1001217 (-) 189 WP_011285559.1 hypothetical protein Regulator
  EW021_RS05155 speA 1001437..1002192 (+) 756 WP_011285560.1 streptococcal pyrogenic exotoxin SpeA -
  EW021_RS05160 - 1002314..1002973 (-) 660 WP_009880240.1 hypothetical protein -
  EW021_RS05165 - 1002973..1003194 (-) 222 WP_009880241.1 hypothetical protein -
  EW021_RS05170 - 1003204..1003977 (-) 774 WP_011054795.1 hypothetical protein -
  EW021_RS05175 - 1003988..1004590 (-) 603 WP_011054796.1 hypothetical protein -
  EW021_RS05180 - 1004602..1005366 (-) 765 WP_011285561.1 CHAP domain-containing protein -
  EW021_RS05185 - 1005368..1005700 (-) 333 WP_011285562.1 phage holin -
  EW021_RS05190 - 1005700..1006023 (-) 324 WP_015055952.1 hypothetical protein -
  EW021_RS09675 - 1006037..1006159 (-) 123 WP_015055953.1 hypothetical protein -
  EW021_RS05195 - 1006173..1006520 (-) 348 WP_011285564.1 DUF1366 domain-containing protein -
  EW021_RS05200 - 1006531..1008393 (-) 1863 WP_015055954.1 DUF859 family phage minor structural protein -
  EW021_RS05205 - 1008398..1011838 (-) 3441 WP_011285566.1 glucosaminidase domain-containing protein -
  EW021_RS05210 - 1011839..1013323 (-) 1485 WP_009880250.1 distal tail protein Dit -
  EW021_RS05215 - 1013324..1015129 (-) 1806 WP_011054802.1 tail protein -
  EW021_RS05220 - 1015122..1015580 (-) 459 WP_009880253.1 hypothetical protein -
  EW021_RS05225 - 1015553..1015870 (-) 318 WP_009880254.1 hypothetical protein -
  EW021_RS05230 - 1015883..1016389 (-) 507 WP_009880255.1 phage major tail protein, TP901-1 family -
  EW021_RS05235 - 1016401..1016811 (-) 411 WP_009880256.1 DUF5072 family protein -
  EW021_RS05240 - 1016813..1017208 (-) 396 WP_009880257.1 hypothetical protein -
  EW021_RS05245 - 1017205..1017516 (-) 312 WP_011285567.1 hypothetical protein -
  EW021_RS05250 - 1017513..1017857 (-) 345 WP_009880259.1 hypothetical protein -
  EW021_RS05255 - 1017871..1018164 (-) 294 WP_009880260.1 HeH/LEM domain-containing protein -
  EW021_RS05260 - 1018177..1019067 (-) 891 WP_009880261.1 hypothetical protein -
  EW021_RS05265 - 1019086..1019655 (-) 570 WP_009880262.1 DUF4355 domain-containing protein -
  EW021_RS09680 - 1019764..1019898 (-) 135 WP_015055956.1 hypothetical protein -
  EW021_RS05270 - 1019900..1020169 (-) 270 WP_011285568.1 hypothetical protein -
  EW021_RS05275 - 1020176..1021084 (-) 909 WP_011285569.1 minor capsid protein -
  EW021_RS05280 - 1021053..1022378 (-) 1326 WP_011285570.1 phage portal protein -
  EW021_RS05285 - 1022378..1023652 (-) 1275 WP_009880266.1 PBSX family phage terminase large subunit -
  EW021_RS05290 - 1023642..1024022 (-) 381 WP_011285571.1 hypothetical protein -
  EW021_RS05295 - 1024632..1025066 (-) 435 WP_011054810.1 ArpU family phage packaging/lysis transcriptional regulator -
  EW021_RS05300 - 1025352..1025618 (-) 267 WP_011018131.1 hypothetical protein -
  EW021_RS05305 - 1025615..1026139 (-) 525 WP_011285572.1 DUF1642 domain-containing protein -
  EW021_RS05310 - 1026142..1026774 (-) 633 WP_011018133.1 N-6 DNA methylase -
  EW021_RS05315 - 1026776..1027060 (-) 285 WP_011018134.1 hypothetical protein -
  EW021_RS09450 - 1027057..1027227 (-) 171 WP_002995952.1 hypothetical protein -
  EW021_RS05320 - 1027224..1027460 (-) 237 WP_002995955.1 DUF3310 domain-containing protein -
  EW021_RS09715 - 1027460..1027705 (-) 246 WP_011285573.1 hypothetical protein -
  EW021_RS05330 - 1027702..1028058 (-) 357 WP_011284873.1 hypothetical protein -
  EW021_RS05335 - 1028055..1028495 (-) 441 WP_011285574.1 RusA family crossover junction endodeoxyribonuclease -
  EW021_RS05340 - 1028495..1028698 (-) 204 WP_011106686.1 hypothetical protein -
  EW021_RS05345 ssb 1028704..1029129 (-) 426 WP_011285575.1 single-stranded DNA-binding protein Machinery gene
  EW021_RS05350 - 1029122..1029796 (-) 675 WP_011285576.1 ERF family protein -
  EW021_RS05355 - 1029797..1030279 (-) 483 WP_011285577.1 siphovirus Gp157 family protein -
  EW021_RS05360 - 1030301..1030555 (-) 255 WP_011285578.1 hypothetical protein -
  EW021_RS05365 - 1030536..1030889 (-) 354 WP_011285579.1 hypothetical protein -
  EW021_RS05370 - 1031030..1031812 (-) 783 WP_011285581.1 ATP-binding protein -
  EW021_RS05375 - 1031799..1032629 (-) 831 WP_009881060.1 phage replisome organizer N-terminal domain-containing protein -
  EW021_RS09605 - 1032643..1032831 (-) 189 Protein_996 XRE family transcriptional regulator -
  EW021_RS09610 - 1033065..1033304 (+) 240 WP_227874181.1 hypothetical protein -
  EW021_RS05390 - 1033435..1033644 (+) 210 WP_011017885.1 hypothetical protein -
  EW021_RS05395 - 1033754..1033954 (-) 201 WP_002992770.1 helix-turn-helix domain-containing protein -
  EW021_RS05400 - 1034028..1034414 (+) 387 WP_011054589.1 hypothetical protein -
  EW021_RS05405 - 1034403..1034612 (-) 210 WP_011284881.1 hypothetical protein -
  EW021_RS05410 - 1034666..1035265 (+) 600 WP_011284882.1 hypothetical protein -
  EW021_RS05415 - 1035295..1035453 (-) 159 WP_011285583.1 hypothetical protein -
  EW021_RS05420 - 1035810..1036634 (+) 825 WP_011285584.1 XRE family transcriptional regulator -
  EW021_RS05425 - 1036670..1037563 (+) 894 WP_011285585.1 P63C domain-containing protein -
  EW021_RS05430 - 1037684..1038772 (+) 1089 WP_011054595.1 site-specific integrase -
  EW021_RS05435 - 1039135..1039755 (+) 621 WP_002989605.1 DUF3862 domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15995.63 Da        Isoelectric Point: 4.5748

>NTDB_id=1116508 EW021_RS05345 WP_011285575.1 1028704..1029129(-) (ssb) [Streptococcus pyogenes strain 5448 isolate 5448]
MINNIVLVGRMTKDAELRYTASQVAVATFTLAVNRRFKEQNGEREADFINCVIWRQSAENLANWAKKGALIGVTGRIQTR
NYENQQEQRVYVTEVVAESFQMLESRNSQQQSGQDNSSQNDNSQPFGNSNPMDISDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1116508 EW021_RS05345 WP_011285575.1 1028704..1029129(-) (ssb) [Streptococcus pyogenes strain 5448 isolate 5448]
ATGATTAACAACATTGTGCTAGTTGGTCGCATGACCAAGGACGCAGAGCTTCGCTATACAGCGAGTCAAGTAGCTGTAGC
TACGTTCACACTTGCGGTAAACCGCAGATTTAAAGAGCAAAACGGGGAGAGAGAAGCAGATTTCATTAACTGTGTTATCT
GGCGACAGTCTGCTGAAAATTTAGCCAACTGGGCTAAAAAAGGTGCTTTGATCGGAGTTACGGGTCGTATTCAGACACGT
AACTACGAAAACCAACAAGAACAACGTGTCTATGTGACGGAAGTTGTTGCAGAGAGTTTCCAAATGTTGGAAAGTCGAAA
TAGCCAGCAACAATCTGGTCAAGATAACTCTTCGCAAAACGATAACAGTCAACCGTTTGGCAATTCAAACCCAATGGATA
TTTCAGACGATGATCTGCCGTTTTAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

55.294

100

0.667
  ssbA Bacillus subtilis subsp. subtilis str. 168

54.07

100

0.66
  ssbB/cilA Streptococcus pneumoniae TIGR4

43.972

100

0.44
  ssbB/cilA Streptococcus pneumoniae Rx1

43.262

100

0.433
  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.262

100

0.433
  ssbB/cilA Streptococcus mitis NCTC 12261

43.262

100

0.433
  ssbB/cilA Streptococcus pneumoniae D39

43.262

100

0.433
  ssbB/cilA Streptococcus pneumoniae R6

43.262

100

0.433
  ssbB/cilA Streptococcus mitis SK321

43.262

100

0.433
  ssbB Bacillus subtilis subsp. subtilis str. 168

51.786

79.433

0.411
  ssbA Streptococcus mutans UA159

40.426

100

0.404
  ssb Glaesserella parasuis strain SC1401

29.379

100

0.369
  ssbB Lactococcus lactis subsp. cremoris KW2

49.524

74.468

0.369

Multiple sequence alignment