Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   EW021_RS00595 Genome accession   NZ_LR130236
Coordinates   95847..96785 (+) Length   312 a.a.
NCBI ID   WP_010921798.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 5448 isolate 5448     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 90847..101785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW021_RS00585 rpoC 91596..95237 (+) 3642 WP_010921797.1 DNA-directed RNA polymerase subunit beta' -
  EW021_RS00590 - 95389..95754 (+) 366 WP_002986560.1 DUF1033 family protein -
  EW021_RS00595 comYA 95847..96785 (+) 939 WP_010921798.1 competence type IV pilus ATPase ComGA Machinery gene
  EW021_RS00600 comYB 96721..97755 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  EW021_RS00605 comYC 97757..98083 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  EW021_RS00610 comGD 98058..98486 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EW021_RS00615 comGE 98443..98727 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  EW021_RS00620 comYF 98720..99154 (+) 435 WP_010921800.1 competence type IV pilus minor pilin ComGF Machinery gene
  EW021_RS00625 comGG 99138..99464 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  EW021_RS00630 comYH 99562..100515 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  EW021_RS00635 - 100574..101770 (+) 1197 WP_010921803.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35068.31 Da        Isoelectric Point: 8.5825

>NTDB_id=1116484 EW021_RS00595 WP_010921798.1 95847..96785(+) (comYA) [Streptococcus pyogenes strain 5448 isolate 5448]
MVQALAKAILAKAEQVHAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1116484 EW021_RS00595 WP_010921798.1 95847..96785(+) (comYA) [Streptococcus pyogenes strain 5448 isolate 5448]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCATGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAAATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.679

0.606
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

39.655

92.949

0.369

Multiple sequence alignment