Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   EW021_RS00620 Genome accession   NZ_LR130236
Coordinates   98720..99154 (+) Length   144 a.a.
NCBI ID   WP_010921800.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 5448 isolate 5448     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 93720..104154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW021_RS00590 - 95389..95754 (+) 366 WP_002986560.1 DUF1033 family protein -
  EW021_RS00595 comYA 95847..96785 (+) 939 WP_010921798.1 competence type IV pilus ATPase ComGA Machinery gene
  EW021_RS00600 comYB 96721..97755 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  EW021_RS00605 comYC 97757..98083 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  EW021_RS00610 comGD 98058..98486 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EW021_RS00615 comGE 98443..98727 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  EW021_RS00620 comYF 98720..99154 (+) 435 WP_010921800.1 competence type IV pilus minor pilin ComGF Machinery gene
  EW021_RS00625 comGG 99138..99464 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  EW021_RS00630 comYH 99562..100515 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  EW021_RS00635 - 100574..101770 (+) 1197 WP_010921803.1 acetate kinase -
  EW021_RS00640 - 101957..102265 (+) 309 WP_010921804.1 hypothetical protein -
  EW021_RS00645 proC 102348..103118 (-) 771 WP_010921805.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16654.28 Da        Isoelectric Point: 10.2526

>NTDB_id=1116487 EW021_RS00620 WP_010921800.1 98720..99154(+) (comYF) [Streptococcus pyogenes strain 5448 isolate 5448]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTRTLLKHSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1116487 EW021_RS00620 WP_010921800.1 98720..99154(+) (comYF) [Streptococcus pyogenes strain 5448 isolate 5448]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCGAACCCTCCTTAAACATAGCCATTATCTAGCCCGTCATGATCAAGATAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGGTTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

47.445

95.139

0.451
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

42.857

92.361

0.396
  comGF/cglF Streptococcus pneumoniae D39

42.857

92.361

0.396
  comGF/cglF Streptococcus pneumoniae R6

42.857

92.361

0.396
  comGF/cglF Streptococcus pneumoniae TIGR4

42.857

92.361

0.396
  comGF/cglF Streptococcus mitis SK321

42.105

92.361

0.389

Multiple sequence alignment