Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   EW021_RS00600 Genome accession   NZ_LR130236
Coordinates   96721..97755 (+) Length   344 a.a.
NCBI ID   WP_011054115.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 5448 isolate 5448     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 91721..102755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW021_RS00590 - 95389..95754 (+) 366 WP_002986560.1 DUF1033 family protein -
  EW021_RS00595 comYA 95847..96785 (+) 939 WP_010921798.1 competence type IV pilus ATPase ComGA Machinery gene
  EW021_RS00600 comYB 96721..97755 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  EW021_RS00605 comYC 97757..98083 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  EW021_RS00610 comGD 98058..98486 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EW021_RS00615 comGE 98443..98727 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  EW021_RS00620 comYF 98720..99154 (+) 435 WP_010921800.1 competence type IV pilus minor pilin ComGF Machinery gene
  EW021_RS00625 comGG 99138..99464 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  EW021_RS00630 comYH 99562..100515 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  EW021_RS00635 - 100574..101770 (+) 1197 WP_010921803.1 acetate kinase -
  EW021_RS00640 - 101957..102265 (+) 309 WP_010921804.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39676.15 Da        Isoelectric Point: 9.7085

>NTDB_id=1116485 EW021_RS00600 WP_011054115.1 96721..97755(+) (comYB) [Streptococcus pyogenes strain 5448 isolate 5448]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1116485 EW021_RS00600 WP_011054115.1 96721..97755(+) (comYB) [Streptococcus pyogenes strain 5448 isolate 5448]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGCCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGGGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.982

98.547

0.532
  comYB Streptococcus mutans UA159

53.982

98.547

0.532
  comYB Streptococcus gordonii str. Challis substr. CH1

50.588

98.837

0.5
  comGB/cglB Streptococcus mitis NCTC 12261

47.619

97.674

0.465
  comGB/cglB Streptococcus mitis SK321

47.748

96.802

0.462
  comGB/cglB Streptococcus pneumoniae Rx1

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae D39

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae R6

45.646

96.802

0.442
  comGB/cglB Streptococcus pneumoniae TIGR4

45.646

96.802

0.442
  comGB Lactococcus lactis subsp. cremoris KW2

42.769

94.477

0.404

Multiple sequence alignment