Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   EQB41_RS10650 Genome accession   NZ_LR129840
Coordinates   1958825..1959766 (-) Length   313 a.a.
NCBI ID   WP_000249567.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 4496 isolate 4496     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1953825..1964766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQB41_RS10610 comYH 1954311..1955264 (-) 954 WP_050237953.1 class I SAM-dependent methyltransferase Machinery gene
  EQB41_RS10615 - 1955325..1955919 (-) 595 Protein_1980 class I SAM-dependent methyltransferase -
  EQB41_RS10620 comGG/cglG 1956056..1956469 (-) 414 WP_050237955.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQB41_RS10625 comGF/cglF 1956447..1956908 (-) 462 WP_127820861.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQB41_RS10630 comGE/cglE 1956871..1957173 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EQB41_RS10635 comGD/cglD 1957136..1957540 (-) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQB41_RS10640 comGC/cglC 1957533..1957859 (-) 327 WP_050237957.1 comG operon protein ComGC Machinery gene
  EQB41_RS10645 comGB/cglB 1957861..1958877 (-) 1017 WP_127820862.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQB41_RS10650 comGA/cglA/cilD 1958825..1959766 (-) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  EQB41_RS10655 - 1959842..1960207 (-) 366 WP_000286415.1 DUF1033 family protein -
  EQB41_RS10660 - 1960358..1961416 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  EQB41_RS10665 nagA 1961579..1962730 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  EQB41_RS10670 - 1962883..1964700 (-) 1818 WP_001220841.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35573.48 Da        Isoelectric Point: 6.0083

>NTDB_id=1116230 EQB41_RS10650 WP_000249567.1 1958825..1959766(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 4496 isolate 4496]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1116230 EQB41_RS10650 WP_000249567.1 1958825..1959766(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 4496 isolate 4496]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCTTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361

Multiple sequence alignment