Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   EQB41_RS10645 Genome accession   NZ_LR129840
Coordinates   1957861..1958877 (-) Length   338 a.a.
NCBI ID   WP_127820862.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 4496 isolate 4496     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1952861..1963877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQB41_RS12675 - 1952938..1953069 (-) 132 WP_000768904.1 hypothetical protein -
  EQB41_RS10605 - 1953070..1954260 (-) 1191 WP_000167765.1 acetate kinase -
  EQB41_RS10610 comYH 1954311..1955264 (-) 954 WP_050237953.1 class I SAM-dependent methyltransferase Machinery gene
  EQB41_RS10615 - 1955325..1955919 (-) 595 Protein_1980 class I SAM-dependent methyltransferase -
  EQB41_RS10620 comGG/cglG 1956056..1956469 (-) 414 WP_050237955.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQB41_RS10625 comGF/cglF 1956447..1956908 (-) 462 WP_127820861.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQB41_RS10630 comGE/cglE 1956871..1957173 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EQB41_RS10635 comGD/cglD 1957136..1957540 (-) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQB41_RS10640 comGC/cglC 1957533..1957859 (-) 327 WP_050237957.1 comG operon protein ComGC Machinery gene
  EQB41_RS10645 comGB/cglB 1957861..1958877 (-) 1017 WP_127820862.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQB41_RS10650 comGA/cglA/cilD 1958825..1959766 (-) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  EQB41_RS10655 - 1959842..1960207 (-) 366 WP_000286415.1 DUF1033 family protein -
  EQB41_RS10660 - 1960358..1961416 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  EQB41_RS10665 nagA 1961579..1962730 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38567.62 Da        Isoelectric Point: 9.7316

>NTDB_id=1116229 EQB41_RS10645 WP_127820862.1 1957861..1958877(-) (comGB/cglB) [Streptococcus pneumoniae strain 4496 isolate 4496]
MDISQVFRLRRKKLATAKQKNIITLFNNFFSSGFHLVETISFLDRSALLDKQCVTRMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMAGLVSVLALLALTFYKRSSKMNVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAILMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1116229 EQB41_RS10645 WP_127820862.1 1957861..1958877(-) (comGB/cglB) [Streptococcus pneumoniae strain 4496 isolate 4496]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATTTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCGGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGTTTTTTCTTCTCTTAATCATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGCAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAATGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATACTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

97.633

100

0.976
  comGB/cglB Streptococcus pneumoniae D39

97.633

100

0.976
  comGB/cglB Streptococcus pneumoniae R6

97.633

100

0.976
  comGB/cglB Streptococcus pneumoniae TIGR4

97.633

100

0.976
  comGB/cglB Streptococcus mitis SK321

93.787

100

0.938
  comGB/cglB Streptococcus mitis NCTC 12261

93.195

100

0.932
  comYB Streptococcus gordonii str. Challis substr. CH1

69.643

99.408

0.692
  comYB Streptococcus mutans UA140

57.413

93.787

0.538
  comYB Streptococcus mutans UA159

57.413

93.787

0.538
  comGB Lactococcus lactis subsp. cremoris KW2

50.299

98.817

0.497

Multiple sequence alignment