Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   EQB41_RS10625 Genome accession   NZ_LR129840
Coordinates   1956447..1956908 (-) Length   153 a.a.
NCBI ID   WP_127820861.1    Uniprot ID   A0A4J2BNJ7
Organism   Streptococcus pneumoniae strain 4496 isolate 4496     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1951447..1961908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQB41_RS10595 - 1951751..1952575 (-) 825 WP_232019722.1 membrane protein insertase YidC -
  EQB41_RS10600 rnpA 1952550..1952921 (-) 372 WP_000739246.1 ribonuclease P protein component -
  EQB41_RS12675 - 1952938..1953069 (-) 132 WP_000768904.1 hypothetical protein -
  EQB41_RS10605 - 1953070..1954260 (-) 1191 WP_000167765.1 acetate kinase -
  EQB41_RS10610 comYH 1954311..1955264 (-) 954 WP_050237953.1 class I SAM-dependent methyltransferase Machinery gene
  EQB41_RS10615 - 1955325..1955919 (-) 595 Protein_1980 class I SAM-dependent methyltransferase -
  EQB41_RS10620 comGG/cglG 1956056..1956469 (-) 414 WP_050237955.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQB41_RS10625 comGF/cglF 1956447..1956908 (-) 462 WP_127820861.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQB41_RS10630 comGE/cglE 1956871..1957173 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EQB41_RS10635 comGD/cglD 1957136..1957540 (-) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQB41_RS10640 comGC/cglC 1957533..1957859 (-) 327 WP_050237957.1 comG operon protein ComGC Machinery gene
  EQB41_RS10645 comGB/cglB 1957861..1958877 (-) 1017 WP_127820862.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQB41_RS10650 comGA/cglA/cilD 1958825..1959766 (-) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  EQB41_RS10655 - 1959842..1960207 (-) 366 WP_000286415.1 DUF1033 family protein -
  EQB41_RS10660 - 1960358..1961416 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17840.36 Da        Isoelectric Point: 6.9873

>NTDB_id=1116225 EQB41_RS10625 WP_127820861.1 1956447..1956908(-) (comGF/cglF) [Streptococcus pneumoniae strain 4496 isolate 4496]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWILFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVRFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1116225 EQB41_RS10625 WP_127820861.1 1956447..1956908(-) (comGF/cglF) [Streptococcus pneumoniae strain 4496 isolate 4496]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
TGTCATCAGTGGGGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGATCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGAGGTTATCAGCCTATGGTTTATGGGCTCAAATCCGCACAGATTACAGAGGACAATCAACTGGTGCGCTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4J2BNJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae D39

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae R6

94.118

100

0.941
  comGF/cglF Streptococcus pneumoniae TIGR4

94.118

100

0.941
  comGF/cglF Streptococcus mitis SK321

92.81

100

0.928
  comGF/cglF Streptococcus mitis NCTC 12261

91.503

100

0.915

Multiple sequence alignment