Detailed information NTG1112676

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KHO05_RS16840
• Genome accession: NZ_HG999364
• Coordinates: 3983136..3984269 (+)
• Length: 377 a.a.
• NCBI ID: WP_104583280.1
• Uniprot ID: -
• Organism: Xanthomonas euroxanthea isolate Xanthomonas euroxanthea CPBF 766 isolated from C. illinoinensis
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3962352..3984948	3983136..3984269	within	0

Gene organization within MGE regions

Location: 3962352..3984948

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KHO05_RS16750 (XCY_003344)	-	3962352..3963302 (+)	951	WP_102580563.1	glycosyltransferase family 2 protein	-
KHO05_RS16755 (XCY_003345)	-	3963299..3963715 (+)	417	WP_164739832.1	GtrA family protein	-
KHO05_RS16760 (XCY_003346)	-	3963690..3965936 (+)	2247	WP_212580197.1	hypothetical protein	-
KHO05_RS16765 (XCY_003347)	-	3966172..3966441 (-)	270	WP_146009944.1	hypothetical protein	-
KHO05_RS16770 (XCY_003348)	-	3966835..3967776 (-)	942	WP_212580198.1	NAD-dependent epimerase/dehydratase family protein	-
KHO05_RS16775 (XCY_003349)	gmd	3967760..3968794 (-)	1035	WP_012439287.1	GDP-mannose 4,6-dehydratase	-
KHO05_RS16780 (XCY_003350)	-	3968872..3969471 (-)	600	WP_206515039.1	glycosyltransferase	-
KHO05_RS16785 (XCY_003351)	-	3969507..3971255 (-)	1749	WP_212581309.1	glycosyltransferase	-
KHO05_RS16790 (XCY_003352)	-	3971276..3972337 (-)	1062	WP_102580568.1	glycosyltransferase family 4 protein	-
KHO05_RS16795 (XCY_003353)	-	3972387..3974675 (-)	2289	WP_241235956.1	methyltransferase domain-containing protein	-
KHO05_RS16800 (XCY_003354)	-	3974715..3976001 (-)	1287	WP_102580569.1	ABC transporter ATP-binding protein	-
KHO05_RS16805 (XCY_003355)	-	3975998..3976783 (-)	786	WP_042827951.1	ABC transporter permease	-
KHO05_RS16810 (XCY_003356)	-	3976876..3978159 (-)	1284	WP_244813180.1	glycosyltransferase family 4 protein	-
KHO05_RS16815 (XCY_003357)	-	3978252..3979451 (-)	1200	WP_104583284.1	cystathionine gamma-synthase	-
KHO05_RS16820 (XCY_003358)	-	3979553..3980923 (-)	1371	WP_212580200.1	pyridoxal-phosphate dependent enzyme	-
KHO05_RS16825 (XCY_003359)	-	3981203..3981421 (-)	219	WP_002814034.1	YdcH family protein	-
KHO05_RS16830 (XCY_003360)	-	3981767..3982657 (-)	891	WP_212580201.1	hypothetical protein	-
KHO05_RS16835 (XCY_003361)	-	3982654..3983037 (-)	384	WP_039525328.1	DUF4398 domain-containing protein	-
KHO05_RS16840 (XCY_003362)	pilU	3983136..3984269 (+)	1134	WP_104583280.1	PilT/PilU family type 4a pilus ATPase	Machinery gene

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41795.16 Da
• Isoelectric Point: 6.1401

>NTDB_id=1112676 KHO05_RS16840 WP_104583280.1 3983136..3984269(+) (pilU) [Xanthomonas euroxanthea isolate Xanthomonas euroxanthea CPBF 766 isolated from C. illinoinensis]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFI

Nucleotide

• Length: 1134 bp

>NTDB_id=1112676 KHO05_RS16840 WP_104583280.1 3983136..3984269(+) (pilU) [Xanthomonas euroxanthea isolate Xanthomonas euroxanthea CPBF 766 isolated from C. illinoinensis]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAGAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGAATGGTCAAGAAAATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGCAAGACGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGTGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATTCCCAGCATCGA
AGAGCTGAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGCTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCATGCCTTCCAGAG
CGCGTTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAGACCATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTTCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCCGTGGTGTCGCTGCG
CCTGGTCAAGGGCGTGGACGGACGCCGCCGGCCGGCGACCGAGGTACTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAAGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGCATGGTCAAGTCCGGCGAAATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGGCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGCTCCGGCGAGCACGACCCGTATGCGGACTACGAAACGGCAGCGGCACCGAAGATCAGCC
ACGGCTTTATCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (74-268)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	52.089	95.225	0.496
pilU	Vibrio cholerae strain A1552	48.889	95.491	0.467
pilU	Acinetobacter baylyi ADP1	49.153	93.899	0.462
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.979	100	0.446
pilT	Pseudomonas aeruginosa PAK	42.308	89.655	0.379
pilT	Legionella pneumophila strain ERS1305867	41.124	89.655	0.369
pilT	Legionella pneumophila strain Lp02	41.124	89.655	0.369
pilT	Acinetobacter baumannii D1279779	40.828	89.655	0.366
pilT	Pseudomonas stutzeri DSM 10701	40.828	89.655	0.366
pilT	Acinetobacter nosocomialis M2	40.828	89.655	0.366
pilT	Acinetobacter baumannii strain A118	40.828	89.655	0.366
pilT	Acinetobacter baylyi ADP1	40.294	90.186	0.363