Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KJB05_RS01940 Genome accession   NZ_HG992742
Coordinates   411230..412978 (+) Length   582 a.a.
NCBI ID   WP_199435956.1    Uniprot ID   A0A812FGU0
Organism   Vibrio sp. B1ASS3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406230..417978
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJB05_RS01920 (ACOMICROBIO_LOCUS260) pilM 408526..409542 (+) 1017 WP_208445806.1 type IV pilus assembly protein PilM Machinery gene
  KJB05_RS01925 (ACOMICROBIO_LOCUS261) pilN 409526..410104 (+) 579 WP_005438427.1 PilN domain-containing protein Machinery gene
  KJB05_RS01930 (ACOMICROBIO_LOCUS262) pilO 410097..410690 (+) 594 WP_039988232.1 type 4a pilus biogenesis protein PilO Machinery gene
  KJB05_RS01935 (ACOMICROBIO_LOCUS263) pilP 410680..411195 (+) 516 WP_005438430.1 pilus assembly protein PilP Machinery gene
  KJB05_RS01940 (ACOMICROBIO_LOCUS264) pilQ 411230..412978 (+) 1749 WP_199435956.1 type IV pilus secretin PilQ family protein Machinery gene
  KJB05_RS01945 (ACOMICROBIO_LOCUS265) aroK 413168..413686 (+) 519 WP_005438434.1 shikimate kinase AroK -
  KJB05_RS01950 (ACOMICROBIO_LOCUS266) aroB 413718..414818 (+) 1101 WP_005438436.1 3-dehydroquinate synthase -
  KJB05_RS01955 (ACOMICROBIO_LOCUS267) - 414872..416365 (+) 1494 WP_208445807.1 AAA family ATPase -
  KJB05_RS01960 (ACOMICROBIO_LOCUS268) - 416435..417274 (+) 840 WP_009705524.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63863.93 Da        Isoelectric Point: 4.5101

>NTDB_id=1112377 KJB05_RS01940 WP_199435956.1 411230..412978(+) (pilQ) [Vibrio sp. B1ASS3]
MKMRQGLTLTPRKLAGIFSLWFFLLLAPFVAAQEVKSNSLKTIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESIEIFRDTSTTRLVAYIDEEYTHDYRLTGRYLEIKVGKIKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGDGEGDDGGSSVGIDDFLNVNLAATSPNA
TSLAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=1112377 KJB05_RS01940 WP_199435956.1 411230..412978(+) (pilQ) [Vibrio sp. B1ASS3]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAGCAGGGATCTTCTCTTTGTGGTTCTTTTTACTTTTAGC
CCCTTTTGTAGCAGCACAGGAAGTAAAGTCTAACTCGCTAAAGACCATCGACTTTCGTACAAACAAAGACAAAGATGCCG
TGATTGTTGTAGAACTTGCGTCCCCTGCTGCAATCGTTGATATCAAGCGCGTACAGGAAGGGTTGAGTATCGACTTACTC
AACACATCTGTAAAAGACGACCAGCTTTACCTTCTGGATGTAAAAGACTTTTCTACGGTCGTCGAGAGTATTGAAATATT
CCGAGATACCTCAACCACACGTTTAGTTGCTTATATTGATGAGGAATACACTCACGATTACCGTCTGACTGGGCGTTACC
TAGAGATCAAAGTCGGTAAGATAAAAGCCAATGAAAAAGCACCGGATAAGAGCATTCTAGAAAAAGAAGGGAAGCTGATT
TCAATTAACTTCCAAGACATCCCAGTGCGAAACGTATTGCAACTCATCGCCGACTACAACCAATTTAACTTAGTGGTATC
TGACTCCGTTGAAGGCAACTTAACTTTGCGTCTTGATGGTGTACCTTGGCAGCAAGTGCTCGATATCATTCTTCAAGTAA
AAGGCTTGGATAAGCGTGTTGATGGCAACGTGATTTTGGTGGCGCCGAAAGACGAACTGGATTTGCGTGAAAAGCAACAA
TTAGAGAAACAGAAACTTGAGCAGGAGATGGGTGAGCTTTCTTCTGAGATCATCAAAGTTAATTTCGCTAAAGCTTCGGA
CATTGCCGAGATGATTAATGGCGAGGGGAATATCAGTATGCTCTCAGAACGTGGCAGCATGACCATTGATGAGCGGACGA
ACTCACTCTTAATTAGAGAACTACCAGAGAACATTGAGGTTATCCGAGAGATCATTGAGTCTTTGGATATTCCGGTCAAA
CAAGTTCAGATTGAAGCGCGAATCGTCACCATCAATGAAGGTAATATGGACGAACTTGGTGTGCGCTGGGGCTTCACCTC
TATTAACGGCAGCCACACTGTCGGTGGTTCGATTGAGAATAACCTTGCGACAATTGGCCTGTACGATGGCGGCGGTGATG
GCGAAGGTGATGACGGTGGCAGCAGTGTTGGTATTGATGACTTCCTAAACGTGAACTTGGCTGCAACCAGTCCGAATGCG
ACCAGTCTTGCCTTCCAAGTTGCGAAATTAGGCTCAGACACACTGCTTGATTTAGAGCTTTCTGCGCTGCAACAAGAATC
CAAGGCAGAGATCATTTCTAGCCCGCGATTGATTACCACTAACAAGAAGCCTGCTTATATCGAGCAAGGTACCGAAATCC
CGTACCTTGAGTCTTCTTCCAGTGGCGCGACGTCTGTCACGTTCAAAAAAGCTGTATTGAGCTTAAAAGTGACACCACAG
ATTACCCCTGACAATCGTTTAGTGTTGGATTTGAGCGTTACGCAAGACAGACCGGGTCAAGTTGTAAAAACAGGCACAGG
TGAAGCCGTCGCGATCGATACTCAACGCATTGGCACACAAGTTTTAGTCAATAACGGTGAAACGGTTGTGTTGGGTGGTA
TTTTTCAACATAGCATCACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTACCGCTGTTAGGGGCACTTTTTCGT
CGAAGTTATGAAAATGTCGGCAAAAGTGAACTGTTGATTTTTGTCACGCCTAAAGTTGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(422-580)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A812FGU0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

96.392

100

0.964
  pilQ Vibrio cholerae strain A1552

76.761

97.595

0.749
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.761

97.595

0.749
  pilQ Pseudomonas aeruginosa PAK

40.522

98.797

0.4
  pilQ Legionella pneumophila strain ERS1305867

40.998

96.392

0.395
  pilQ Legionella pneumophila strain Lp02

40.998

96.392

0.395
  pilQ Acinetobacter baumannii D1279779

36.728

100

0.378
  pilQ Acinetobacter baumannii strain A118

36.728

100

0.378
  comQ Acinetobacter baylyi ADP1

37.177

99.828

0.371

Multiple sequence alignment