Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KJB05_RS01920 Genome accession   NZ_HG992742
Coordinates   408526..409542 (+) Length   338 a.a.
NCBI ID   WP_208445806.1    Uniprot ID   A0A812FDU7
Organism   Vibrio sp. B1ASS3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 403526..414542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJB05_RS01910 (ACOMICROBIO_LOCUS258) - 404341..405720 (+) 1380 WP_213053098.1 IS4 family transposase -
  KJB05_RS01915 (ACOMICROBIO_LOCUS259) - 405777..408347 (-) 2571 WP_208445805.1 PBP1A family penicillin-binding protein -
  KJB05_RS01920 (ACOMICROBIO_LOCUS260) pilM 408526..409542 (+) 1017 WP_208445806.1 type IV pilus assembly protein PilM Machinery gene
  KJB05_RS01925 (ACOMICROBIO_LOCUS261) pilN 409526..410104 (+) 579 WP_005438427.1 PilN domain-containing protein Machinery gene
  KJB05_RS01930 (ACOMICROBIO_LOCUS262) pilO 410097..410690 (+) 594 WP_039988232.1 type 4a pilus biogenesis protein PilO Machinery gene
  KJB05_RS01935 (ACOMICROBIO_LOCUS263) pilP 410680..411195 (+) 516 WP_005438430.1 pilus assembly protein PilP Machinery gene
  KJB05_RS01940 (ACOMICROBIO_LOCUS264) pilQ 411230..412978 (+) 1749 WP_199435956.1 type IV pilus secretin PilQ family protein Machinery gene
  KJB05_RS01945 (ACOMICROBIO_LOCUS265) aroK 413168..413686 (+) 519 WP_005438434.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37988.28 Da        Isoelectric Point: 6.0145

>NTDB_id=1112373 KJB05_RS01920 WP_208445806.1 408526..409542(+) (pilM) [Vibrio sp. B1ASS3]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKERIVAENNTINHQEIVKTLKEMRKELPIRARKVAISVPDSA
VISKKLQIEQNLEESEKEFAVMQAFAHQSPFPVEELSLDFVKVDTDEQMGGTESYQVFATRREVVESRVDTLTKSGLKTV
LVDVHSQSLGHIWKLTAERFPEKNKHCLLDIGAMSSSFTMFTEQGELFHKEFPCGTRTTNDLSQHDPLNDDIQTATEEFN
RQMVERVKRQMQLYTSFNGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLSLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDDAASH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1112373 KJB05_RS01920 WP_208445806.1 408526..409542(+) (pilM) [Vibrio sp. B1ASS3]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCTTTATTGGGTTACAAAGAAATAATACTTAAGGAACGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAGAAAAGAGCTCCCAATTCGAGCTAGAAAGGTCGCAATTTCGGTTCCAGACAGTGCT
GTGATCAGCAAAAAGCTACAAATAGAGCAGAACCTAGAAGAAAGCGAAAAGGAATTTGCGGTGATGCAAGCCTTCGCGCA
TCAGTCTCCCTTTCCGGTTGAAGAGCTCAGCCTCGATTTTGTGAAGGTTGATACGGATGAGCAGATGGGCGGCACAGAGA
GCTACCAAGTGTTTGCAACACGCAGAGAGGTGGTGGAAAGCCGAGTGGATACATTAACGAAGTCTGGCCTAAAAACCGTG
CTGGTGGATGTGCACTCACAAAGTCTTGGTCATATATGGAAGCTAACCGCAGAACGTTTTCCAGAAAAGAATAAGCATTG
CTTGTTAGACATTGGTGCGATGAGTAGCTCTTTCACTATGTTTACCGAGCAAGGTGAGCTATTCCATAAGGAGTTTCCTT
GCGGTACTCGCACAACAAACGACTTGTCTCAACACGATCCACTCAATGACGATATTCAAACCGCAACTGAGGAGTTCAAT
CGACAAATGGTTGAACGTGTTAAGCGTCAGATGCAGCTCTATACCTCGTTCAATGGCAGCCAAGATATCAAAGGCATTTG
GCTTTCCGGTGAGGGGGCGGCTACGCCAATGTTAGCTGAGGAGCTATCAAGTCAATTGGCGCTGGAGTGTGAACTGCTCA
ATCCTCTTAGTTTGTTTGAAATGAAAGTCTCTAAACGTAAACGCCGAGCTGCAGATTGGCAGCACTTCACGGCAGCCGCA
GGTTTAGCGGTACGCGGTATTCAGTGGTTGGGAGGTGACGATGCTGCATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A812FDU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

88.462

100

0.885
  pilM Vibrio cholerae strain A1552

48.276

100

0.497

Multiple sequence alignment