Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KJB05_RS01930 Genome accession   NZ_HG992742
Coordinates   410097..410690 (+) Length   197 a.a.
NCBI ID   WP_039988232.1    Uniprot ID   -
Organism   Vibrio sp. B1ASS3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 405097..415690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJB05_RS01915 (ACOMICROBIO_LOCUS259) - 405777..408347 (-) 2571 WP_208445805.1 PBP1A family penicillin-binding protein -
  KJB05_RS01920 (ACOMICROBIO_LOCUS260) pilM 408526..409542 (+) 1017 WP_208445806.1 type IV pilus assembly protein PilM Machinery gene
  KJB05_RS01925 (ACOMICROBIO_LOCUS261) pilN 409526..410104 (+) 579 WP_005438427.1 PilN domain-containing protein Machinery gene
  KJB05_RS01930 (ACOMICROBIO_LOCUS262) pilO 410097..410690 (+) 594 WP_039988232.1 type 4a pilus biogenesis protein PilO Machinery gene
  KJB05_RS01935 (ACOMICROBIO_LOCUS263) pilP 410680..411195 (+) 516 WP_005438430.1 pilus assembly protein PilP Machinery gene
  KJB05_RS01940 (ACOMICROBIO_LOCUS264) pilQ 411230..412978 (+) 1749 WP_199435956.1 type IV pilus secretin PilQ family protein Machinery gene
  KJB05_RS01945 (ACOMICROBIO_LOCUS265) aroK 413168..413686 (+) 519 WP_005438434.1 shikimate kinase AroK -
  KJB05_RS01950 (ACOMICROBIO_LOCUS266) aroB 413718..414818 (+) 1101 WP_005438436.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23076.66 Da        Isoelectric Point: 5.1105

>NTDB_id=1112375 KJB05_RS01930 WP_039988232.1 410097..410690(+) (pilO) [Vibrio sp. B1ASS3]
MVNWQDLEMDEIAEWPLVPQLVVLLMLVFLIQGFGYWFYLKPEQARLDNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVKANTYQFKPEEKKDAK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=1112375 KJB05_RS01930 WP_039988232.1 410097..410690(+) (pilO) [Vibrio sp. B1ASS3]
ATGGTTAATTGGCAAGACCTTGAAATGGATGAAATCGCTGAATGGCCACTGGTGCCGCAGTTAGTCGTTCTCTTAATGCT
CGTGTTTCTGATTCAAGGTTTCGGTTACTGGTTCTATTTAAAGCCAGAGCAAGCGCGTTTGGACAATTTGATTCAAGAAG
AGCAAACCTTAAAAGTTGCGCTGAGAATCAAGGCCAATAAAGTGGCGGCTTTGCCTCAACTGCAATCACAACTCGATGAG
CTCACCACTCGCTACGAACTGCTACTGCAACAGCTACCTGCGCAAAAAGAGCTGGCCACCATGCTGGCTTCGGTGAATGA
ACTGGGCATCGATAACAAACTGACATTTACCCGCATTGACTGGGGGGAGAAACAGTCAGAGCACTTCCTTTATCGTCTGC
CGCTGAATATTGAACTCACTGGAGACTTTCATGATATCGGCCGTTTCTCTCAAGCCATCGCCACATTGCCTCGCATCATC
ACGTTTAAAGACGTGACTTGGCAACGAGTGAGTCAAGAAAGTGAAACTTTGCACTTCCGTGTAAAAGCGAATACCTACCA
GTTCAAACCAGAGGAAAAGAAAGATGCGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

93.158

96.447

0.898
  pilO Vibrio cholerae strain A1552

71.728

96.954

0.695

Multiple sequence alignment