Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   P3C55_RS18645 Genome accession   NZ_HG992342
Coordinates   4357385..4358518 (+) Length   377 a.a.
NCBI ID   WP_016900968.1    Uniprot ID   A0A2N7V1C6
Organism   Xanthomonas arboricola pv. corylina strain CFBP 6600 isolate K100-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4337577..4363477 4357385..4358518 within 0


Gene organization within MGE regions


Location: 4337577..4363477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3C55_RS18555 (CFBP6600_37040) - 4337577..4338305 (+) 729 WP_104613211.1 SDR family oxidoreductase -
  P3C55_RS18560 (CFBP6600_37050) - 4338307..4339251 (+) 945 WP_104567020.1 NAD(P)-dependent oxidoreductase -
  P3C55_RS18565 (CFBP6600_37060) - 4339274..4340569 (+) 1296 WP_104595056.1 NAD(P)/FAD-dependent oxidoreductase -
  P3C55_RS18570 (CFBP6600_37070) - 4340566..4340958 (+) 393 WP_039513631.1 GtrA family protein -
  P3C55_RS18575 (CFBP6600_37080) - 4341076..4342515 (+) 1440 WP_104613212.1 hypothetical protein -
  P3C55_RS18580 (CFBP6600_37090) - 4342792..4343319 (-) 528 WP_146091488.1 hypothetical protein -
  P3C55_RS18585 (CFBP6600_37100) - 4343348..4344943 (-) 1596 WP_146091489.1 hypothetical protein -
  P3C55_RS18590 (CFBP6600_37110) - 4345026..4346000 (-) 975 WP_104613214.1 glycosyltransferase -
  P3C55_RS18595 (CFBP6600_37120) - 4345997..4348513 (-) 2517 WP_104613215.1 glycosyltransferase -
  P3C55_RS18600 (CFBP6600_37130) - 4348572..4349669 (-) 1098 WP_243849943.1 class I SAM-dependent methyltransferase -
  P3C55_RS18605 (CFBP6600_37140) - 4349708..4350289 (-) 582 WP_104595051.1 CatB-related O-acetyltransferase -
  P3C55_RS18610 (CFBP6600_37150) - 4350286..4351626 (-) 1341 WP_104613216.1 ABC transporter ATP-binding protein -
  P3C55_RS18615 (CFBP6600_37160) - 4351666..4352487 (-) 822 WP_104613217.1 ABC transporter permease -
  P3C55_RS18620 (CFBP6600_37170) - 4352484..4353704 (-) 1221 WP_104613218.1 cystathionine gamma-synthase -
  P3C55_RS18625 (CFBP6600_37180) - 4353794..4355164 (-) 1371 WP_046344410.1 pyridoxal-phosphate dependent enzyme -
  P3C55_RS18630 (CFBP6600_37190) - 4355452..4355670 (-) 219 WP_002814034.1 YdcH family protein -
  P3C55_RS18635 (CFBP6600_37200) - 4356016..4356906 (-) 891 WP_039814588.1 membrane protein -
  P3C55_RS18640 (CFBP6600_37210) - 4356903..4357286 (-) 384 WP_039525328.1 DUF4398 domain-containing protein -
  P3C55_RS18645 (CFBP6600_37220) pilU 4357385..4358518 (+) 1134 WP_016900968.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P3C55_RS18650 (CFBP6600_37230) maiA 4358802..4359467 (-) 666 WP_039512969.1 maleylacetoacetate isomerase -
  P3C55_RS18655 (CFBP6600_37240) - 4359511..4360569 (-) 1059 WP_172454165.1 fumarylacetoacetate hydrolase family protein -
  P3C55_RS18660 (CFBP6600_37250) - 4360878..4362266 (+) 1389 WP_016900971.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41754.11 Da        Isoelectric Point: 6.1401

>NTDB_id=1112369 P3C55_RS18645 WP_016900968.1 4357385..4358518(+) (pilU) [Xanthomonas arboricola pv. corylina strain CFBP 6600 isolate K100-1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1112369 P3C55_RS18645 WP_016900968.1 4357385..4358518(+) (pilU) [Xanthomonas arboricola pv. corylina strain CFBP 6600 isolate K100-1]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCACCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGATTGCCGCCGGGAATGGTCAAGAAAATCGCTTATTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATTGCCCTGTCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCCAGCATCGA
AGAACTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGCTCGACCGGCT
CGGGCAAATCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACCATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTGTCGCTGCG
CCTGGTCAAGGGTGTGGACGGACGCCGCCGGCCGGCGACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAAGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGCATGGTCAAGTCCGGCGAAATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGCTCCGGCGAGCACGACCCGTACGCGGATTACGAAACGGCAGCAGCGCCGAAGATCAGCC
ACGGCTTTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7V1C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

48.889

95.491

0.467
  pilU Acinetobacter baylyi ADP1

49.153

93.899

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.979

100

0.446
  pilT Pseudomonas aeruginosa PAK

42.308

89.655

0.379
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

40.828

89.655

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366
  pilT Acinetobacter nosocomialis M2

40.828

89.655

0.366
  pilT Acinetobacter baumannii D1279779

40.828

89.655

0.366
  pilT Acinetobacter baylyi ADP1

40.294

90.186

0.363

Multiple sequence alignment