Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   P3C56_RS18750 Genome accession   NZ_HG992338
Coordinates   4397673..4398806 (+) Length   377 a.a.
NCBI ID   WP_016900968.1    Uniprot ID   A0A2N7V1C6
Organism   Xanthomonas arboricola pv. corylina strain 301 isolate 301     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4377865..4404023 4397673..4398806 within 0


Gene organization within MGE regions


Location: 4377865..4404023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3C56_RS18660 (XAC301_37130) - 4377865..4378593 (+) 729 WP_104613211.1 SDR family oxidoreductase -
  P3C56_RS18665 (XAC301_37140) - 4378595..4379539 (+) 945 WP_104567020.1 NAD-dependent epimerase/dehydratase family protein -
  P3C56_RS18670 (XAC301_37150) - 4379562..4380857 (+) 1296 WP_275548142.1 NAD(P)/FAD-dependent oxidoreductase -
  P3C56_RS18675 (XAC301_37160) - 4380854..4381246 (+) 393 WP_039513631.1 GtrA family protein -
  P3C56_RS18680 (XAC301_37170) - 4381328..4382803 (+) 1476 WP_146092679.1 hypothetical protein -
  P3C56_RS18685 (XAC301_37180) - 4383080..4383607 (-) 528 WP_146091488.1 hypothetical protein -
  P3C56_RS18690 (XAC301_37190) - 4383636..4385231 (-) 1596 WP_146091489.1 hypothetical protein -
  P3C56_RS18695 (XAC301_37200) - 4385314..4386288 (-) 975 WP_212583584.1 glycosyltransferase -
  P3C56_RS18700 (XAC301_37210) - 4386285..4388801 (-) 2517 WP_104613215.1 glycosyltransferase -
  P3C56_RS18705 (XAC301_37220) - 4388860..4389957 (-) 1098 WP_275548143.1 class I SAM-dependent methyltransferase -
  P3C56_RS18710 (XAC301_37230) - 4389996..4390577 (-) 582 WP_104595051.1 CatB-related O-acetyltransferase -
  P3C56_RS18715 (XAC301_37240) - 4390574..4391914 (-) 1341 WP_104613216.1 ABC transporter ATP-binding protein -
  P3C56_RS18720 (XAC301_37250) - 4391954..4392775 (-) 822 WP_039513645.1 ABC transporter permease -
  P3C56_RS18725 (XAC301_37260) - 4392772..4393992 (-) 1221 WP_104595049.1 cystathionine gamma-synthase -
  P3C56_RS18730 (XAC301_37270) - 4394082..4395452 (-) 1371 WP_212583585.1 pyridoxal-phosphate dependent enzyme -
  P3C56_RS18735 (XAC301_37280) - 4395740..4395958 (-) 219 WP_002814034.1 YdcH family protein -
  P3C56_RS18740 (XAC301_37290) - 4396304..4397194 (-) 891 WP_016900966.1 membrane protein -
  P3C56_RS18745 (XAC301_37300) - 4397191..4397574 (-) 384 WP_039525328.1 DUF4398 domain-containing protein -
  P3C56_RS18750 (XAC301_37310) pilU 4397673..4398806 (+) 1134 WP_016900968.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P3C56_RS18755 (XAC301_37320) maiA 4399090..4399755 (-) 666 WP_047130637.1 maleylacetoacetate isomerase -
  P3C56_RS18760 (XAC301_37330) - 4399799..4400857 (-) 1059 WP_186007775.1 fumarylacetoacetate hydrolase family protein -
  P3C56_RS18765 (XAC301_37340) - 4401166..4402554 (+) 1389 WP_016900971.1 DEAD/DEAH box helicase -
  P3C56_RS18770 (XAC301_37350) - 4402680..4404023 (+) 1344 WP_169751462.1 IS4 family transposase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41754.11 Da        Isoelectric Point: 6.1401

>NTDB_id=1112336 P3C56_RS18750 WP_016900968.1 4397673..4398806(+) (pilU) [Xanthomonas arboricola pv. corylina strain 301 isolate 301]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1112336 P3C56_RS18750 WP_016900968.1 4397673..4398806(+) (pilU) [Xanthomonas arboricola pv. corylina strain 301 isolate 301]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCACCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGAATGGTCAAGAAAATCGCTTATTCAT
TGATGGACGAAGGCCAGGTACCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATTGCCCTGTCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTTGTGGTCGGCTCGACCGGCT
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGACACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAAAG
CGCGCTGAAAAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCCGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTGTCGTTGCG
CCTGGTCAAGGGTGTGGACGGACGCCGCCGGCCTGCGACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GCCGTGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGCATGGTCAAGTCCGGCGAAATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGTTCCGGCGAGCACGACCCGTACGCAGATTACGAAACGGCAGCAGCGCCGAAGATCAGCC
ACGGCTTTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7V1C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

48.889

95.491

0.467
  pilU Acinetobacter baylyi ADP1

49.153

93.899

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.979

100

0.446
  pilT Pseudomonas aeruginosa PAK

42.308

89.655

0.379
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

40.828

89.655

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366
  pilT Acinetobacter nosocomialis M2

40.828

89.655

0.366
  pilT Acinetobacter baumannii D1279779

40.828

89.655

0.366
  pilT Acinetobacter baylyi ADP1

40.294

90.186

0.363

Multiple sequence alignment