Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LOK40_RS17160 Genome accession   NZ_HG992336
Coordinates   4064224..4065357 (+) Length   377 a.a.
NCBI ID   WP_228607883.1    Uniprot ID   -
Organism   Xanthomonas arboricola strain 1311a isolate 1311a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4059224..4070357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOK40_RS17135 (XA1311A_33510) - 4059344..4060543 (-) 1200 WP_127158824.1 cystathionine gamma-synthase -
  LOK40_RS17140 (XA1311A_33520) - 4060633..4062003 (-) 1371 WP_047127302.1 pyridoxal-phosphate dependent enzyme -
  LOK40_RS17145 (XA1311A_33530) - 4062291..4062509 (-) 219 WP_002814034.1 YdcH family protein -
  LOK40_RS17150 (XA1311A_33540) - 4062855..4063745 (-) 891 WP_016900966.1 membrane protein -
  LOK40_RS17155 (XA1311A_33550) - 4063742..4064125 (-) 384 WP_039525328.1 DUF4398 domain-containing protein -
  LOK40_RS17160 (XA1311A_33560) pilU 4064224..4065357 (+) 1134 WP_228607883.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LOK40_RS17165 (XA1311A_33570) maiA 4065640..4066305 (-) 666 WP_053051187.1 maleylacetoacetate isomerase -
  LOK40_RS17170 (XA1311A_33580) - 4066349..4067407 (-) 1059 WP_026064014.1 fumarylacetoacetate hydrolase family protein -
  LOK40_RS17175 (XA1311A_33590) - 4067716..4069104 (+) 1389 WP_024938371.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41768.13 Da        Isoelectric Point: 6.1406

>NTDB_id=1112302 LOK40_RS17160 WP_228607883.1 4064224..4065357(+) (pilU) [Xanthomonas arboricola strain 1311a isolate 1311a]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSEAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1112302 LOK40_RS17160 WP_228607883.1 4064224..4065357(+) (pilU) [Xanthomonas arboricola strain 1311a isolate 1311a]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCACCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGAATGGTCAAGAAAATCGCTTATTCAT
TGATGGACGAAGGCCAGGTACCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATTGCCCTGTCCGAGGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTTGTGGTCGGCTCGACCGGCT
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGACACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAAAG
CGCGCTGAAAAACGCGATGCGCGAGGCCCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCCGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTGTCGTTGCG
CCTGGTCAAGGGTGTGGACGGACGCCGCCGGCCTGCGACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GCCGTGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGCATGGTCAAGTCCGGCGAAATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGTTCCGGCGAGCACGACCCGTACGCGGATTACGAAACGGCAGCGGCACCGAAGATCAGCC
ACGGCTTTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilU Vibrio cholerae strain A1552

48.611

95.491

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.717

100

0.443
  pilT Pseudomonas aeruginosa PAK

42.308

89.655

0.379
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baumannii D1279779

40.828

89.655

0.366
  pilT Acinetobacter nosocomialis M2

40.828

89.655

0.366
  pilT Acinetobacter baumannii strain A118

40.828

89.655

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.018

88.594

0.363
  pilT Acinetobacter baylyi ADP1

40.294

90.186

0.363

Multiple sequence alignment