Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   HMPREF9425_RS00675 Genome accession   NZ_GL831112
Coordinates   131980..132921 (+) Length   313 a.a.
NCBI ID   WP_003095883.1    Uniprot ID   -
Organism   Streptococcus vestibularis ATCC 49124     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 126980..137921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMPREF9425_RS00665 (HMPREF9425_0137) rpoC 127737..131375 (+) 3639 WP_037621242.1 DNA-directed RNA polymerase subunit beta' -
  HMPREF9425_RS00670 (HMPREF9425_0138) - 131537..131899 (+) 363 WP_003095881.1 DUF1033 family protein -
  HMPREF9425_RS00675 (HMPREF9425_0139) comYA 131980..132921 (+) 942 WP_003095883.1 competence type IV pilus ATPase ComGA Machinery gene
  HMPREF9425_RS00680 (HMPREF9425_0140) comYB 132803..133903 (+) 1101 WP_155809878.1 competence type IV pilus assembly protein ComGB Machinery gene
  HMPREF9425_RS00685 (HMPREF9425_0141) comYC 133900..134226 (+) 327 WP_002885815.1 competence type IV pilus major pilin ComGC Machinery gene
  HMPREF9425_RS00690 (HMPREF9425_0142) comYD 134186..134614 (+) 429 WP_253665936.1 competence type IV pilus minor pilin ComGD Machinery gene
  HMPREF9425_RS00695 (HMPREF9425_0143) comGE 134646..134876 (+) 231 WP_003095891.1 competence type IV pilus minor pilin ComGE -
  HMPREF9425_RS00700 (HMPREF9425_0144) comYF 134863..135300 (+) 438 WP_003091908.1 competence type IV pilus minor pilin ComGF Machinery gene
  HMPREF9425_RS00705 (HMPREF9425_0145) comGG 135278..135595 (+) 318 WP_003092009.1 competence type IV pilus minor pilin ComGG -
  HMPREF9425_RS00710 (HMPREF9425_0146) comYH 135640..136596 (+) 957 WP_003091902.1 class I SAM-dependent methyltransferase Machinery gene
  HMPREF9425_RS00715 (HMPREF9425_0147) - 136653..137846 (+) 1194 WP_003095894.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35202.22 Da        Isoelectric Point: 5.9265

>NTDB_id=1111718 HMPREF9425_RS00675 WP_003095883.1 131980..132921(+) (comYA) [Streptococcus vestibularis ATCC 49124]
MITEFAKEIIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRVLHSERRELTYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDNLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSVAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGAIDFACENFQSHSSASWNQQLEGLVKQGYLTEGDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1111718 HMPREF9425_RS00675 WP_003095883.1 131980..132921(+) (comYA) [Streptococcus vestibularis ATCC 49124]
ATGATAACAGAATTTGCTAAAGAAATAATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTTGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTAGGTGAGAAGCGTAGGAGTCAATTGGGCTCTTGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATTCGTGT
TCTTCATTCCGAACGAAGAGAGCTAACTTACTGGAATCAAGGGATTCAGCCTATTAAGGATGCATTGGATTATCGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAATTGGTTCAGGAGCGTTTTAAGGGACAG
CAGGTGATTTCTATTGAAGATCCTGTGGAAATTAAACAGGATAATGTCTTGCAACTCCAGGTAAATCAGGCAATTGACAT
GACCTATGATAATTTGATTAAGCTATCACTACGTCACCGCCCGGATGTTTTGATTATTGGAGAAATTCGAGATAAGGAGA
CTGCTCGAGCGGTTATTAGAGCTAGTCTGACAGGAGTGACTGTTCTTTCAACTATTCACGCAAAGAGTGTGGCAGGTGTT
TATGAGCGTCTTCTGGACCTCGGTGTAGATAAGTCTGAGTTGGATAATGCTCTTCAAGGGATTGCCTATATGCGTTTGAT
TAAGGGAGGAGGTGCGATTGATTTTGCCTGTGAAAATTTCCAAAGCCATTCGTCAGCCAGCTGGAACCAACAGTTGGAAG
GCTTGGTTAAACAAGGATATCTCACTGAAGGGGATATCCAAGGGGAAAAAATTAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

65.176

100

0.652
  comYA Streptococcus mutans UA140

65.176

100

0.652
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.595

99.361

0.652
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.63

99.361

0.642
  comYA Streptococcus gordonii str. Challis substr. CH1

61.981

100

0.62
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.577

99.681

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

50.804

99.361

0.505

Multiple sequence alignment