Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNOHK_RS18995 Genome accession   NZ_CP184332
Coordinates   4318671..4319804 (+) Length   377 a.a.
NCBI ID   WP_012439300.1    Uniprot ID   Q93S76
Organism   Xanthomonas campestris pv. campestris isolate BJSJQ20200612-pXX3-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4313671..4324804
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNOHK_RS18970 (ACNOHK_18970) - 4313848..4315047 (-) 1200 WP_075287759.1 cystathionine gamma-synthase -
  ACNOHK_RS18975 (ACNOHK_18975) - 4315141..4316511 (-) 1371 WP_050911712.1 pyridoxal-phosphate dependent enzyme -
  ACNOHK_RS18980 (ACNOHK_18980) - 4316743..4316961 (-) 219 WP_019237985.1 YdcH family protein -
  ACNOHK_RS18985 (ACNOHK_18985) - 4317302..4318192 (-) 891 WP_011035839.1 membrane protein -
  ACNOHK_RS18990 (ACNOHK_18990) - 4318189..4318572 (-) 384 WP_011035838.1 DUF4398 domain-containing protein -
  ACNOHK_RS18995 (ACNOHK_18995) pilU 4318671..4319804 (+) 1134 WP_012439300.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNOHK_RS19000 (ACNOHK_19000) maiA 4320324..4320998 (-) 675 WP_011035836.1 maleylacetoacetate isomerase -
  ACNOHK_RS19005 (ACNOHK_19005) - 4321042..4322031 (-) 990 WP_011035835.1 fumarylacetoacetate hydrolase family protein -
  ACNOHK_RS19010 (ACNOHK_19010) - 4322411..4323799 (+) 1389 WP_014506542.1 DEAD/DEAH box helicase -
  ACNOHK_RS19015 (ACNOHK_19015) queD 4323915..4324268 (+) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  ACNOHK_RS19020 (ACNOHK_19020) - 4324458..4324793 (+) 336 WP_115000044.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41799.10 Da        Isoelectric Point: 6.1401

>NTDB_id=1108259 ACNOHK_RS18995 WP_012439300.1 4318671..4319804(+) (pilU) [Xanthomonas campestris pv. campestris isolate BJSJQ20200612-pXX3-4]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1108259 ACNOHK_RS18995 WP_012439300.1 4318671..4319804(+) (pilU) [Xanthomonas campestris pv. campestris isolate BJSJQ20200612-pXX3-4]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTTTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCTCTGTCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGTT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCCGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAGACCATGGAGGCGG
CGATCGCCTTCGCTGAAACCGGCCATCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTACTGATGAACCTGGCACTGAATCTGCGCGCAGTGGTGTCCCAGCG
CCTGGTCAAGGGACTGGACGGGCGCCGCCGCCCGGCCACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGGCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCTTGGCGCTGAA
ATTCCGTCTTTCCGAAGGCGGCTCCGGCGAGCACGATCCGTATGCCGACTACGAGTCGGCCTCGGCGCCGAAGATCAGCC
ATGGGTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q93S76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.018

88.594

0.363

Multiple sequence alignment