Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACGJAX_RS01920 Genome accession   NZ_AP035776
Coordinates   403845..404264 (-) Length   139 a.a.
NCBI ID   WP_390957292.1    Uniprot ID   -
Organism   Pseudomonas moorei strain m318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 398845..409264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGJAX_RS01905 (PMm318_A03650) - 398929..399945 (+) 1017 WP_090325220.1 class I SAM-dependent rRNA methyltransferase -
  ACGJAX_RS01910 (PMm318_A03660) - 400039..402993 (+) 2955 WP_390957291.1 DUF748 domain-containing protein -
  ACGJAX_RS01915 (PMm318_A03670) - 403183..403536 (-) 354 WP_090325225.1 BON domain-containing protein -
  ACGJAX_RS01920 (PMm318_A03680) pilA 403845..404264 (-) 420 WP_390957292.1 pilin Machinery gene
  ACGJAX_RS01925 (PMm318_A03690) pilC 404685..405902 (+) 1218 WP_390957293.1 type II secretion system F family protein Machinery gene
  ACGJAX_RS01930 (PMm318_A03700) pilD 405904..406776 (+) 873 WP_248915556.1 A24 family peptidase Machinery gene
  ACGJAX_RS01935 (PMm318_A03710) coaE 406773..407396 (+) 624 WP_390957294.1 dephospho-CoA kinase -
  ACGJAX_RS01940 (PMm318_A03720) yacG 407393..407593 (+) 201 WP_248915558.1 DNA gyrase inhibitor YacG -
  ACGJAX_RS01945 (PMm318_A03730) - 407603..407818 (-) 216 WP_047533370.1 hypothetical protein -
  ACGJAX_RS01950 (PMm318_A03740) - 407882..408571 (-) 690 WP_247254694.1 energy-coupling factor ABC transporter permease -
  ACGJAX_RS01955 (PMm318_A03750) - 408568..409035 (-) 468 WP_390957295.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14315.49 Da        Isoelectric Point: 7.5886

>NTDB_id=110764 ACGJAX_RS01920 WP_390957292.1 403845..404264(-) (pilA) [Pseudomonas moorei strain m318]
MNQQKGFTLIELLIVVAIIGILATFAIPAYSKYQARAKVTAGLAEISALKVPFEDVINQGDDPDLKTVGGTPTTSNCTTT
ASGKAADGTGTIACTIVNAPGPVLNKTITLTRTLTGWTCATTVDQDYAPKGCTGVAPKV

Nucleotide


Download         Length: 420 bp        

>NTDB_id=110764 ACGJAX_RS01920 WP_390957292.1 403845..404264(-) (pilA) [Pseudomonas moorei strain m318]
ATGAACCAACAAAAAGGCTTTACCCTGATTGAGCTACTGATCGTGGTCGCAATCATCGGGATTCTGGCAACGTTCGCGAT
TCCTGCGTATTCCAAATACCAGGCCCGGGCCAAGGTGACGGCGGGGCTGGCGGAGATTTCGGCCTTGAAGGTGCCGTTCG
AAGATGTCATCAATCAAGGCGACGATCCGGATCTGAAGACGGTGGGTGGCACCCCGACCACCTCCAATTGCACAACGACA
GCGTCCGGTAAAGCGGCGGATGGCACAGGCACTATTGCCTGCACAATCGTGAATGCACCCGGCCCGGTGCTGAACAAAAC
CATTACCCTGACGCGTACCCTGACGGGGTGGACGTGCGCCACCACGGTCGATCAGGACTATGCGCCCAAAGGCTGTACGG
GCGTCGCCCCGAAAGTCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

47.887

100

0.489
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.905

98.561

0.482
  pilA Pseudomonas aeruginosa PAK

41.611

100

0.446
  comP Acinetobacter baylyi ADP1

40.789

100

0.446
  pilA Vibrio cholerae strain A1552

43.571

100

0.439
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.571

100

0.439
  pilA Vibrio cholerae C6706

43.571

100

0.439
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.704

97.122

0.424
  pilA2 Legionella pneumophila strain ERS1305867

41.429

100

0.417
  pilA2 Legionella pneumophila str. Paris

40.876

98.561

0.403

Multiple sequence alignment