Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACN08M_RS04900 Genome accession   NZ_CP183250
Coordinates   997261..997650 (+) Length   129 a.a.
NCBI ID   WP_045068531.1    Uniprot ID   A0A2T3MCZ5
Organism   Photobacterium leiognathi subsp. mandapamensis strain Sc16.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 992261..1002650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN08M_RS04885 (ACN08M_04885) pdhR 994135..994908 (-) 774 WP_008987925.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACN08M_RS04890 (ACN08M_04890) ampD 995230..995775 (-) 546 WP_419239397.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACN08M_RS04895 (ACN08M_04895) nadC 996026..996913 (+) 888 WP_419240159.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACN08M_RS04900 (ACN08M_04900) pilA 997261..997650 (+) 390 WP_045068531.1 pilin Machinery gene
  ACN08M_RS04905 (ACN08M_04905) pilB 997654..999339 (+) 1686 WP_419239398.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACN08M_RS04910 (ACN08M_04910) pilC 999384..1000607 (+) 1224 WP_419239399.1 type II secretion system F family protein Machinery gene
  ACN08M_RS04915 (ACN08M_04915) pilD 1000696..1001565 (+) 870 WP_419239400.1 prepilin peptidase Machinery gene
  ACN08M_RS04920 (ACN08M_04920) coaE 1001569..1002174 (+) 606 WP_318444194.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 129 a.a.        Molecular weight: 12883.78 Da        Isoelectric Point: 9.0498

>NTDB_id=1104846 ACN08M_RS04900 WP_045068531.1 997261..997650(+) (pilA) [Photobacterium leiognathi subsp. mandapamensis strain Sc16.3]
MKKQQGFTLIELMIVVAVIGVLSAIAIPQYQKYVQKGALASALASATALKTNYEDYVAVSGAAPTQASDIGAVSFSLGTI
GVTSGAITANITKGGGKDNSIVLTRSSGGSWSCAISGAAASDVKLNGCP

Nucleotide


Download         Length: 390 bp        

>NTDB_id=1104846 ACN08M_RS04900 WP_045068531.1 997261..997650(+) (pilA) [Photobacterium leiognathi subsp. mandapamensis strain Sc16.3]
ATGAAAAAACAACAGGGTTTTACTCTTATTGAATTGATGATCGTGGTGGCGGTTATCGGTGTGCTTTCGGCAATTGCTAT
TCCACAATATCAGAAATATGTACAAAAAGGAGCATTAGCTTCTGCGTTAGCTTCTGCAACTGCTTTAAAAACTAACTACG
AAGATTATGTTGCAGTTAGTGGTGCAGCACCAACACAAGCATCTGACATTGGAGCTGTATCTTTTAGCCTTGGAACAATT
GGAGTTACTTCAGGTGCTATTACAGCAAACATTACAAAAGGTGGCGGTAAAGATAATAGTATCGTTTTAACACGATCTTC
AGGCGGTAGCTGGTCATGTGCTATTAGCGGAGCAGCTGCATCAGATGTTAAACTAAACGGCTGTCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(39-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3MCZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.366

100

0.488
  pilA Vibrio cholerae strain A1552

44.366

100

0.488
  pilA Vibrio cholerae C6706

44.366

100

0.488
  pilA Pseudomonas aeruginosa PAK

44.444

100

0.465
  pilA Vibrio campbellii strain DS40M4

37.584

100

0.434
  pilA Acinetobacter baumannii strain A118

44.8

96.899

0.434
  pilA Vibrio parahaemolyticus RIMD 2210633

39.13

100

0.419
  pilA2 Legionella pneumophila str. Paris

40.65

95.349

0.388
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.062

99.225

0.388
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.843

93.798

0.364

Multiple sequence alignment