Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACNI65_RS24760 Genome accession   NZ_CP182288
Coordinates   5430569..5431012 (+) Length   147 a.a.
NCBI ID   WP_416760127.1    Uniprot ID   -
Organism   Roseateles sp. So40a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5425569..5436012
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNI65_RS24735 (ACNI65_24735) - 5425962..5426693 (+) 732 WP_416760118.1 TerC family protein -
  ACNI65_RS24740 (ACNI65_24740) - 5426712..5427782 (+) 1071 WP_416760119.1 serine/threonine-protein kinase -
  ACNI65_RS24745 (ACNI65_24745) - 5427779..5428573 (+) 795 WP_416760120.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ACNI65_RS24750 (ACNI65_24750) - 5428614..5429270 (+) 657 WP_416760122.1 FHA domain-containing protein -
  ACNI65_RS24755 (ACNI65_24755) - 5429481..5430263 (+) 783 WP_416760124.1 MBL fold metallo-hydrolase -
  ACNI65_RS24760 (ACNI65_24760) pilE 5430569..5431012 (+) 444 WP_416760127.1 pilin Machinery gene
  ACNI65_RS24765 (ACNI65_24765) - 5431138..5432574 (+) 1437 WP_416760129.1 O-antigen ligase family protein -
  ACNI65_RS24770 (ACNI65_24770) - 5432574..5433350 (+) 777 WP_416760131.1 pilus assembly protein -
  ACNI65_RS24775 (ACNI65_24775) - 5433437..5435251 (+) 1815 WP_416760132.1 Wzy polymerase domain-containing protein -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15287.69 Da        Isoelectric Point: 9.0011

>NTDB_id=1101000 ACNI65_RS24760 WP_416760127.1 5430569..5431012(+) (pilE) [Roseateles sp. So40a]
MKRVQQGFTLIELMIVVAIIGILAAVALPAYSDYIKKSKVSEVVGVASQPKAAIQEWLSVKGSFPADQATLGLEFNPTKY
VSAMTYTGGTTAVLTATAQAIGTGVDANNIVLTGTRVTNGGTLTDQVTWACTGTIQAKYLPASCKGT

Nucleotide


Download         Length: 444 bp        

>NTDB_id=1101000 ACNI65_RS24760 WP_416760127.1 5430569..5431012(+) (pilE) [Roseateles sp. So40a]
ATGAAGCGCGTTCAACAAGGTTTCACCCTGATCGAACTGATGATCGTCGTGGCGATCATCGGCATCCTGGCTGCCGTGGC
CCTGCCGGCCTACTCGGACTACATCAAGAAGTCCAAGGTTTCGGAAGTCGTTGGCGTTGCCAGCCAGCCGAAGGCCGCCA
TTCAGGAATGGCTGTCGGTCAAGGGCTCGTTCCCGGCTGACCAAGCCACGCTGGGCCTGGAATTCAACCCGACCAAGTAC
GTGTCGGCGATGACCTACACCGGCGGCACCACCGCTGTCCTGACCGCCACGGCTCAAGCCATCGGCACCGGCGTGGACGC
CAACAACATCGTCCTGACCGGCACCCGCGTCACCAACGGCGGCACGCTGACCGACCAAGTGACCTGGGCCTGCACGGGCA
CCATCCAGGCCAAGTACCTGCCGGCTTCGTGCAAGGGCACCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.368

100

0.483
  pilA2 Legionella pneumophila str. Paris

45.07

96.599

0.435
  pilA2 Legionella pneumophila strain ERS1305867

44.366

96.599

0.429
  pilE Neisseria gonorrhoeae strain FA1090

37.805

100

0.422
  comP Acinetobacter baylyi ADP1

40.523

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

35.028

100

0.422
  pilE Neisseria gonorrhoeae MS11

35.714

100

0.408
  pilA Vibrio campbellii strain DS40M4

37.255

100

0.388
  comE Acinetobacter baylyi ADP1

35

100

0.381
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.424

100

0.374
  pilA Acinetobacter baumannii strain A118

35.762

100

0.367
  pilA/pilA1 Eikenella corrodens VA1

34.615

100

0.367

Multiple sequence alignment