Detailed information NTG1096110

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMXYI_RS25235
• Genome accession: NZ_CP180481
• Coordinates: 5509723..5510832 (-)
• Length: 369 a.a.
• NCBI ID: WP_415769562.1
• Uniprot ID: -
• Organism: Pseudomonas sp. LB3P38
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5504723..5515832

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMXYI_RS25220	-	5505298..5506689 (+)	1392	WP_415769559.1	GntP family permease	-
ACMXYI_RS25225	-	5506697..5507482 (+)	786	WP_415769560.1	3-hydroxybutyrate dehydrogenase	-
ACMXYI_RS25230	-	5507640..5509595 (+)	1956	WP_415769561.1	acetoacetate--CoA ligase	-
ACMXYI_RS25235	pilU	5509723..5510832 (-)	1110	WP_415769562.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMXYI_RS25240	-	5510953..5513490 (+)	2538	WP_415769563.1	PAS domain S-box protein	-
ACMXYI_RS25245	-	5513543..5513818 (+)	276	WP_007898282.1	peptidylprolyl isomerase	-
ACMXYI_RS25250	-	5513830..5514765 (-)	936	WP_415773981.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41152.07 Da
• Isoelectric Point: 6.4546

>NTDB_id=1096110 ACMXYI_RS25235 WP_415769562.1 5509723..5510832(-) (pilU) [Pseudomonas sp. LB3P38]
MEIDALLRLLASQNGSDLYLSTGAPPSARFEGVLKPLADQPFKPGEVAAIAASIMDAEQRLEFDRELEMNLAISLTGVGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPPVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHTALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRTQLLHDLGNNLKAFVSQRLVHTRDGRRRAAVEVMIGTPTIGDLIRRNEFSELKGIMEKSVEQGMQTFDGAL
FELVAEGAIDEEEALKHADSVNNLRLRLKLHAEASPGPHAPPGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=1096110 ACMXYI_RS25235 WP_415769562.1 5509723..5510832(-) (pilU) [Pseudomonas sp. LB3P38]
ATGGAAATCGATGCACTGTTAAGGCTCCTGGCCAGCCAGAATGGCTCCGATCTTTATCTGTCTACCGGTGCGCCACCCAG
CGCACGGTTCGAGGGTGTGCTCAAACCCTTGGCCGATCAGCCGTTCAAACCGGGAGAAGTTGCGGCCATTGCCGCGTCCA
TCATGGACGCCGAACAGCGTCTGGAGTTCGACCGGGAGCTGGAGATGAACCTGGCCATCTCTTTGACCGGTGTCGGGCGC
TTTCGGGTCAATATCTTCAAACAACGCAACGATGTGTCTATCGTGGCGCGCAATATCAAACTCGACATTCCGCGATTCGA
AGACCTCAAGTTGCCACCGGTGCTGCTCGAAACGGTGATGCTCAAACAAGGACTGATGCTGTTCGTCGGCGCTACCGACT
CGGGCAAGTCAACTTCGCTGGCCGCGTTGATCGACTACCGCAATCGCCACAGCAGCGGCCACATCATCACCATTGAAGAC
CCGGTCGAATACATCCATCGGCACAAGAAGTCGATCATCAACCAGCGTGAGGTCGGCGTCGATACCCGCAGTTTTCACAC
CGCCTTGAAAAACACCCTGCGCCAGGCACCTGACGTGGTGCTGATCGGCGAAATCCGTGACCGCGAGACGATGGAGCATG
CGCTGGCGTTTGCCGATACCGGTCATCTGGTGATTTCCACCCTGCATGCCCACAACGCCCATCAGGCGCTGGACCGGATT
ATCAACTTCTTCCCCGAAGAACGCCGGACGCAGTTGCTTCATGATCTGGGCAACAACCTCAAGGCGTTCGTGTCCCAGCG
GTTGGTACACACCCGGGACGGTCGGCGACGGGCGGCGGTGGAAGTGATGATCGGCACGCCGACCATTGGCGATCTGATCC
GGCGCAATGAGTTTTCCGAACTCAAGGGGATTATGGAGAAGTCTGTGGAGCAGGGGATGCAGACGTTTGACGGTGCGCTA
TTCGAGTTGGTCGCAGAGGGGGCTATCGATGAGGAGGAAGCGTTGAAGCATGCGGATTCGGTGAACAATTTGCGCCTGCG
GCTGAAGCTGCACGCCGAAGCATCACCGGGCCCCCACGCACCGCCGGGTGAATGGGGACTGATGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.978	99.729	0.558
pilU	Acinetobacter baylyi ADP1	52.941	96.748	0.512
pilU	Vibrio cholerae strain A1552	50	94.851	0.474
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.315	99.729	0.382
pilT	Pseudomonas aeruginosa PAK	40.698	93.225	0.379
pilT	Pseudomonas stutzeri DSM 10701	40.407	93.225	0.377
pilT	Vibrio cholerae strain A1552	41.441	90.244	0.374
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.441	90.244	0.374
pilT	Legionella pneumophila strain ERS1305867	40.419	90.515	0.366
pilT	Legionella pneumophila strain Lp02	40.419	90.515	0.366
pilT	Neisseria gonorrhoeae MS11	38.841	93.496	0.363
pilT	Neisseria meningitidis 8013	39.296	92.412	0.363