Detailed information NTG1096074

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMXYJ_RS14725
• Genome accession: NZ_CP180480
• Coordinates: 3130945..3132054 (+)
• Length: 369 a.a.
• NCBI ID: WP_415760405.1
• Uniprot ID: -
• Organism: Pseudomonas sp. LT1P18
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3125945..3137054

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMXYJ_RS14710	-	3126929..3127909 (+)	981	WP_415760403.1	sugar kinase	-
ACMXYJ_RS14715	-	3127943..3128218 (-)	276	WP_007898282.1	peptidylprolyl isomerase	-
ACMXYJ_RS14720	-	3128287..3130824 (-)	2538	WP_415760404.1	PAS domain S-box protein	-
ACMXYJ_RS14725	pilU	3130945..3132054 (+)	1110	WP_415760405.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMXYJ_RS14730	-	3132170..3134125 (-)	1956	WP_415760406.1	acetoacetate--CoA ligase	-
ACMXYJ_RS14735	hbdH	3134287..3135060 (-)	774	WP_415760407.1	3-hydroxybutyrate dehydrogenase	-
ACMXYJ_RS14740	-	3135068..3136459 (-)	1392	WP_415760408.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41105.16 Da
• Isoelectric Point: 6.6717

>NTDB_id=1096074 ACMXYJ_RS14725 WP_415760405.1 3130945..3132054(+) (pilU) [Pseudomonas sp. LT1P18]
MEIDALLRLLASQNGSDLYLSTGAPPSARFEGVLKPLADQPFKPGEVAAIAASIMDAEQRLEFDRELEMNLAISLPGVGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPPVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRTQLLHDLGNNLKAFVSQRLVHTRNGQRRAAVEVMLGTPTIGDLIRRNEFSELKGIMEKSVELGMQTFDCAL
YALVVEGAINEEEALKHADSVNNLRLRLKLHAEASPGPHAPPGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=1096074 ACMXYJ_RS14725 WP_415760405.1 3130945..3132054(+) (pilU) [Pseudomonas sp. LT1P18]
ATGGAAATCGATGCACTGTTAAGGCTCCTGGCCAGCCAGAATGGCTCCGATCTTTATCTGTCTACCGGTGCGCCACCCAG
CGCACGGTTCGAGGGTGTGCTCAAGCCTTTGGCCGATCAGCCGTTCAAACCGGGAGAAGTCGCGGCCATTGCCGCGTCCA
TCATGGACGCCGAACAGCGTCTGGAGTTCGACCGGGAGCTGGAGATGAACCTGGCCATCTCTTTGCCTGGTGTCGGGCGC
TTTCGGGTCAATATCTTCAAACAACGCAACGATGTGTCGATCGTGGCGCGCAACATCAAGCTCGACATTCCGCGTTTCGA
AGACCTCAAGCTGCCGCCGGTGCTGCTCGAAACGGTGATGCTCAAACAAGGACTGATGCTGTTCGTCGGCGCTACCGACT
CGGGCAAGTCAACCTCGCTGGCGGCATTGATCGACTACCGCAATCGCCACAGCAGTGGCCACATCATCACCATCGAAGAC
CCGGTCGAGTACATCCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGCGTCGATACCCGCAGTTTTCACGC
CGCATTGAAAAATACCCTGCGCCAGGCACCGGACGTCGTGCTGATCGGCGAAATTCGTGACCGCGAGACGATGGAGCATG
CACTGGCGTTTGCCGATACCGGTCATCTGGTTATCTCCACGCTGCATGCGCACAACGCCCATCAGGCGCTGGACCGGATT
ATCAACTTCTTTCCAGAGGAGCGTCGCACGCAGTTGCTTCATGATCTGGGCAACAACCTGAAAGCGTTCGTGTCTCAGCG
CCTGGTGCATACCCGCAACGGTCAGCGACGGGCGGCGGTGGAGGTGATGCTGGGTACGCCGACCATTGGCGATCTGATCC
GGCGCAATGAGTTTTCAGAACTGAAGGGGATCATGGAGAAGTCTGTGGAGCTGGGCATGCAGACGTTTGACTGTGCACTT
TACGCGCTGGTCGTCGAGGGGGCGATCAATGAGGAAGAAGCGTTGAAGCATGCGGATTCGGTGAACAATTTGCGCCTGCG
GCTGAAGCTGCACGCCGAAGCATCACCAGGCCCCCATGCACCGCCGGGTGAATGGGGACTCATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.25	99.729	0.561
pilU	Acinetobacter baylyi ADP1	52.661	96.748	0.509
pilU	Vibrio cholerae strain A1552	51.143	94.851	0.485
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.587	99.729	0.385
pilT	Pseudomonas aeruginosa PAK	40.698	93.225	0.379
pilT	Pseudomonas stutzeri DSM 10701	40.407	93.225	0.377
pilT	Vibrio cholerae strain A1552	41.441	90.244	0.374
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.441	90.244	0.374
pilT	Legionella pneumophila strain Lp02	40.419	90.515	0.366
pilT	Legionella pneumophila strain ERS1305867	40.419	90.515	0.366
pilT	Neisseria gonorrhoeae MS11	38.551	93.496	0.36
pilT	Neisseria meningitidis 8013	38.551	93.496	0.36