Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACMGF5_RS10715 Genome accession   NZ_CP179898
Coordinates   2329677..2330945 (+) Length   422 a.a.
NCBI ID   WP_115273375.1    Uniprot ID   -
Organism   Pseudomonas sp. BNK-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2324677..2335945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMGF5_RS10695 (ACMGF5_10695) - 2324724..2327174 (-) 2451 WP_410003547.1 penicillin-binding protein 1A -
  ACMGF5_RS10700 (ACMGF5_10700) pilM 2327356..2328204 (+) 849 WP_115273373.1 type IV pilus biogenesis protein PilM -
  ACMGF5_RS10705 (ACMGF5_10705) - 2328204..2328740 (+) 537 WP_115273374.1 PilN domain-containing protein -
  ACMGF5_RS10710 (ACMGF5_10710) - 2328737..2329753 (+) 1017 WP_204919413.1 pilus assembly protein PilP -
  ACMGF5_RS10715 (ACMGF5_10715) pilQ 2329677..2330945 (+) 1269 WP_115273375.1 type IV pilus secretin PilQ Machinery gene
  ACMGF5_RS10720 (ACMGF5_10720) aroK 2330950..2331468 (+) 519 WP_039602073.1 shikimate kinase AroK -
  ACMGF5_RS10725 (ACMGF5_10725) aroB 2331563..2332660 (+) 1098 WP_046787053.1 3-dehydroquinate synthase -
  ACMGF5_RS10730 (ACMGF5_10730) - 2332676..2334271 (+) 1596 WP_046787054.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46048.03 Da        Isoelectric Point: 4.8797

>NTDB_id=1093551 ACMGF5_RS10715 WP_115273375.1 2329677..2330945(+) (pilQ) [Pseudomonas sp. BNK-15]
MNRLMKWVVAALLPMPLLAATPYRGEPISLNFQDVEVRLVLQVLADYAGINLVASDTVQGSITLRLQDVPWDQALELVLR
SKGLARREEGKVLLVAPAAELAEQALGARIGLELDAQSQPLRRELLPIHHAEASELAELLLATLADDGILTGRGSLNVDV
RTNTLVAHQPEERLAELRQLVAQLDVPVRQVEIEARIVEANVDYEKSLGVRWGPRLYSEAAVLGKDVFVDLGVERAGSSI
GLGVLRGSVLLDLELSAMEKTGNGEIISQPKVVTANKETARILKGTEVPYQETTKSGATSVAFREASLSLEVTPQITPDG
KVIMTVKVTKDEPDFVNALNDVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMNDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=1093551 ACMGF5_RS10715 WP_115273375.1 2329677..2330945(+) (pilQ) [Pseudomonas sp. BNK-15]
ATGAACAGGTTGATGAAGTGGGTGGTGGCGGCCTTGCTGCCGATGCCGTTGCTGGCGGCAACGCCTTATCGGGGTGAGCC
GATTTCGCTGAACTTCCAGGACGTGGAAGTGCGCCTGGTGTTGCAGGTGCTGGCCGATTATGCCGGCATCAACCTGGTCG
CCAGCGACACGGTGCAGGGCAGTATTACCTTGCGTCTGCAGGATGTTCCCTGGGATCAGGCTTTGGAGCTGGTGTTGCGC
AGCAAGGGCCTGGCCCGGCGCGAAGAGGGCAAGGTGCTGTTGGTGGCGCCAGCGGCTGAGCTGGCCGAACAAGCCCTGGG
CGCGCGTATAGGCTTGGAGCTGGACGCCCAATCGCAGCCGTTGCGCCGCGAGTTGCTGCCGATACACCACGCCGAGGCCT
CAGAGCTTGCCGAATTGCTGCTGGCCACGCTGGCCGATGACGGCATTCTGACGGGGCGCGGTAGTTTGAATGTGGATGTG
CGCACCAATACGCTGGTGGCGCACCAGCCTGAGGAGCGGCTTGCCGAGCTGCGCCAGTTGGTGGCGCAACTGGATGTGCC
GGTACGGCAGGTAGAGATCGAGGCGCGCATCGTCGAGGCCAATGTCGATTACGAGAAAAGCCTTGGGGTGCGCTGGGGCC
CGCGGCTCTACAGCGAAGCTGCTGTTTTGGGCAAGGACGTGTTTGTCGATCTTGGGGTGGAACGTGCCGGTTCCAGCATT
GGCTTGGGGGTGTTGCGTGGGAGCGTGCTGCTGGACCTTGAACTCAGCGCCATGGAGAAGACCGGCAATGGTGAAATCAT
CTCCCAGCCCAAGGTGGTCACCGCCAATAAGGAAACCGCCCGCATCCTCAAAGGCACCGAAGTCCCTTACCAGGAGACCA
CGAAGAGTGGCGCTACCTCGGTTGCATTCCGCGAGGCTTCGCTGTCGTTGGAGGTCACTCCGCAGATTACGCCGGACGGC
AAGGTGATCATGACGGTCAAGGTGACCAAGGACGAGCCTGATTTCGTCAATGCCTTGAACGATGTGCCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTACGGGTAGCTGACGGGGAAACCATCGTCATTGGCGGTGTGTATTCGACCACGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGTGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAATGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Bacterial type II and III secretion system protein (Secretin)

(257-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.957

100

0.616
  comQ Acinetobacter baylyi ADP1

42.316

100

0.45
  pilQ Acinetobacter baumannii D1279779

40.733

100

0.448
  pilQ Acinetobacter baumannii strain A118

40.733

100

0.448
  pilQ Legionella pneumophila strain Lp02

42.755

99.763

0.427
  pilQ Legionella pneumophila strain ERS1305867

42.755

99.763

0.427
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.381

99.526

0.422
  pilQ Vibrio cholerae strain A1552

42.381

99.526

0.422
  pilQ Vibrio campbellii strain DS40M4

40.61

100

0.41
  pilQ Neisseria gonorrhoeae strain FA1090

37.5

100

0.377
  pilQ Neisseria meningitidis MC58

36.407

100

0.365
  pilQ Neisseria meningitidis M1080

36.407

100

0.365

Multiple sequence alignment