Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ACJWMD_RS09585 Genome accession   NZ_CP177182
Coordinates   2008731..2009672 (-) Length   313 a.a.
NCBI ID   WP_002887018.1    Uniprot ID   A0AAW6D6G7
Organism   Streptococcus salivarius strain MRD6060     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2003731..2014672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJWMD_RS09545 - 2003806..2004999 (-) 1194 WP_002885831.1 acetate kinase -
  ACJWMD_RS09550 comYH 2005056..2006012 (-) 957 WP_002887012.1 class I SAM-dependent methyltransferase Machinery gene
  ACJWMD_RS09555 comGG 2006057..2006374 (-) 318 WP_002887013.1 competence type IV pilus minor pilin ComGG -
  ACJWMD_RS09560 comYF 2006352..2006789 (-) 438 WP_002887014.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACJWMD_RS09565 comGE 2006776..2007006 (-) 231 WP_002887015.1 competence type IV pilus minor pilin ComGE -
  ACJWMD_RS09570 comYD 2007038..2007466 (-) 429 WP_254593949.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACJWMD_RS09575 comYC 2007426..2007740 (-) 315 WP_170314557.1 competence type IV pilus major pilin ComGC Machinery gene
  ACJWMD_RS09580 comYB 2007749..2008849 (-) 1101 WP_139053029.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACJWMD_RS09585 comGA/cglA/cilD 2008731..2009672 (-) 942 WP_002887018.1 competence type IV pilus ATPase ComGA Machinery gene
  ACJWMD_RS09590 - 2009751..2010113 (-) 363 WP_002887019.1 DUF1033 family protein -
  ACJWMD_RS09595 rpoC 2010276..2013914 (-) 3639 WP_002887020.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35283.31 Da        Isoelectric Point: 5.7041

>NTDB_id=1086041 ACJWMD_RS09585 WP_002887018.1 2008731..2009672(-) (comGA/cglA/cilD) [Streptococcus salivarius strain MRD6060]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGQRVSLRLSTVGDYRGLESLVIRVLHSERRELVYWNQGIQPIMDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDNLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSVAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFASENFQSHSSTSWNQQLEGLVKQGYLTEGDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1086041 ACJWMD_RS09585 WP_002887018.1 2008731..2009672(-) (comGA/cglA/cilD) [Streptococcus salivarius strain MRD6060]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTTGGCCAGGAGAGGAGATTAATTGATGTATATCGACCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTAGGTTCCTGTGACTATGAT
TGTGGTGATGGTCAAAGAGTTTCTCTGCGTTTGTCTACCGTTGGGGATTATCGTGGCTTAGAAAGTTTAGTTATTCGTGT
TCTACATTCCGAACGTCGAGAATTAGTGTACTGGAATCAAGGAATCCAGCCTATTATGGATGCTTTGGATTATCGTGGAT
TATATCTCTTTGCAGGTCCCGTTGGTTCTGGAAAGACTACGCTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTGATTTCGATTGAGGACCCTGTGGAAATTAAACAGGATAATGTCTTGCAACTCCAGGTGAATCAAGCAATTGACAT
GACCTATGATAATTTGATTAAGCTATCACTGCGTCACCGCCCGGATGTTTTGATTATTGGAGAGATTCGAGATAAGGAGA
CTGCTCGAGCGGTTATTAGAGCTAGTCTGACGGGAGTGACTGTTCTTTCAACTATTCACGCAAAGAGTGTGGCAGGTGTT
TATGAACGTCTTCTGGACCTTGGTGTAGATAAGTCTGAGTTGGATAATGCCCTTCAAGGGATTGCCTACATGCGTCTGAT
TAAGGGAGGAGGTGTGATTGATTTTGCCAGTGAAAATTTCCAAAGCCATTCGTCAACCAGCTGGAACCAGCAGTTGGAAG
GCTTGGTTAAACAAGGATATCTCACTGAAGGGGATATCCAAGGGGAAAAAATTAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.916

99.361

0.655
  comYA Streptococcus mutans UA159

65.176

100

0.652
  comYA Streptococcus mutans UA140

65.176

100

0.652
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.63

99.361

0.642
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.897

99.681

0.607
  comGA Lactococcus lactis subsp. cremoris KW2

51.447

99.361

0.511
  comGA Latilactobacillus sakei subsp. sakei 23K

41.697

86.581

0.361

Multiple sequence alignment