Detailed information NTG1086037

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: ACJWMD_RS09560
• Genome accession: NZ_CP177182
• Coordinates: 2006352..2006789 (-)
• Length: 145 a.a.
• NCBI ID: WP_002887014.1
• Uniprot ID: A0A1V0GF29
• Organism: Streptococcus salivarius strain MRD6060
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2001352..2011789

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJWMD_RS09525	-	2001395..2002057 (-)	663	WP_002887009.1	CPBP family intramembrane glutamic endopeptidase	-
ACJWMD_RS09530	-	2002170..2002616 (-)	447	WP_037605837.1	hypothetical protein	-
ACJWMD_RS09535	-	2002690..2003346 (-)	657	WP_002887011.1	CPBP family intramembrane glutamic endopeptidase	-
ACJWMD_RS09540	-	2003358..2003555 (-)	198	WP_002885716.1	helix-turn-helix transcriptional regulator	-
ACJWMD_RS09545	-	2003806..2004999 (-)	1194	WP_002885831.1	acetate kinase	-
ACJWMD_RS09550	comYH	2005056..2006012 (-)	957	WP_002887012.1	class I SAM-dependent methyltransferase	Machinery gene
ACJWMD_RS09555	comGG	2006057..2006374 (-)	318	WP_002887013.1	competence type IV pilus minor pilin ComGG	-
ACJWMD_RS09560	comYF	2006352..2006789 (-)	438	WP_002887014.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACJWMD_RS09565	comGE	2006776..2007006 (-)	231	WP_002887015.1	competence type IV pilus minor pilin ComGE	-
ACJWMD_RS09570	comYD	2007038..2007466 (-)	429	WP_254593949.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACJWMD_RS09575	comYC	2007426..2007740 (-)	315	WP_170314557.1	competence type IV pilus major pilin ComGC	Machinery gene
ACJWMD_RS09580	comYB	2007749..2008849 (-)	1101	WP_139053029.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACJWMD_RS09585	comGA/cglA/cilD	2008731..2009672 (-)	942	WP_002887018.1	competence type IV pilus ATPase ComGA	Machinery gene
ACJWMD_RS09590	-	2009751..2010113 (-)	363	WP_002887019.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16155.57 Da
• Isoelectric Point: 8.4658

>NTDB_id=1086037 ACJWMD_RS09560 WP_002887014.1 2006352..2006789(-) (comYF) [Streptococcus salivarius strain MRD6060]
MSLNSKVRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVKQQESQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKASTISQNNQIVTIQVTLKNGLQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=1086037 ACJWMD_RS09560 WP_002887014.1 2006352..2006789(-) (comYF) [Streptococcus salivarius strain MRD6060]
ATGTCTCTCAATAGCAAGGTTCGTGCTTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTGATTGCTGGTTCAGTTCA
AGTTTATCAAGGTCTGACCACAGTATTGGTGAGCAATGTTAAGCAGGTCAAGCAACAAGAAAGCCAAGACTGGCTTCTAT
TCGTTCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTAGCTTTTGGACTATCAAGTGCTACTGATTTTCGAAAAACAAATGCTGACGGTCGAGGCTACCAACCCAT
GCTTTTTAATGTAAAAGCTAGTACAATTAGCCAAAATAACCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A1V0GF29

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae Rx1	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae D39	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae R6	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50	97.931	0.49
comGF/cglF	Streptococcus mitis SK321	47.222	99.31	0.469
comGF/cglF	Streptococcus mitis NCTC 12261	47.222	99.31	0.469
comGF	Lactococcus lactis subsp. cremoris KW2	45.324	95.862	0.434