Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACK3CJ_RS20715 Genome accession   NZ_CP176580
Coordinates   4502078..4503250 (-) Length   390 a.a.
NCBI ID   WP_409524090.1    Uniprot ID   -
Organism   Nitrincola sp. WZQS7-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4497078..4508250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK3CJ_RS20690 - 4498014..4498598 (+) 585 WP_409524085.1 YqgE/AlgH family protein -
  ACK3CJ_RS20695 ruvX 4498619..4499077 (+) 459 WP_409524086.1 Holliday junction resolvase RuvX -
  ACK3CJ_RS20700 pyrR 4499077..4499586 (+) 510 WP_409524087.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACK3CJ_RS20705 - 4499612..4500616 (+) 1005 WP_409524088.1 aspartate carbamoyltransferase catalytic subunit -
  ACK3CJ_RS20710 - 4500633..4501916 (+) 1284 WP_409524089.1 dihydroorotase -
  ACK3CJ_RS20715 pilU 4502078..4503250 (-) 1173 WP_409524090.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACK3CJ_RS20720 pilT 4503271..4504305 (-) 1035 WP_409524091.1 type IV pilus twitching motility protein PilT Machinery gene
  ACK3CJ_RS20725 - 4504390..4505088 (+) 699 WP_409524092.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACK3CJ_RS20730 proC 4505122..4505949 (+) 828 WP_409524093.1 pyrroline-5-carboxylate reductase -
  ACK3CJ_RS20735 - 4506061..4506603 (+) 543 WP_409524094.1 YggT family protein -
  ACK3CJ_RS20740 - 4506671..4507843 (+) 1173 WP_409524095.1 homoserine O-acetyltransferase -

Sequence

Protein


Download         Length: 390 a.a.        Molecular weight: 43023.45 Da        Isoelectric Point: 6.0097

>NTDB_id=1081062 ACK3CJ_RS20715 WP_409524090.1 4502078..4503250(-) (pilU) [Nitrincola sp. WZQS7-5]
MDFTQLLKVMSDRGASDLFVTAGAAPAIKVDGTIKPLTKESLKPAQARALVYSTMNDKQLAEFEGTHECNFAISAPGIGR
FRVSAFIQRNTPGMVLRRIDTYIPSLEELNLPPVLKDLSMTKRGLILFVGGTSTGKSTSLASMIDYRNENYAGHIITIED
PIEYIHNHKKSIITQREVGLDTDSFEIALKNTLRQAPDVILMGEIRSMETMKYGLQFAETGHLCMATLHANNANQALDRI
ISFFPADHHDQLWMDLSLNLKAIVAQQLLPTVDGKGRRAAIEVLINTPLIKDLIRKADVHEIKDVMKKSTNLGMQTFDQS
LFKLYKEGVIAYDVALTHADSANDLRLMIKLDGSTAVPEGKGGEGDMPSFTLQDEEDNLNISGSVDVKAM

Nucleotide


Download         Length: 1173 bp        

>NTDB_id=1081062 ACK3CJ_RS20715 WP_409524090.1 4502078..4503250(-) (pilU) [Nitrincola sp. WZQS7-5]
ATGGACTTTACACAACTGCTTAAGGTGATGTCTGATCGCGGGGCGTCGGATCTCTTCGTGACGGCCGGTGCTGCACCGGC
TATCAAGGTGGATGGTACGATCAAGCCGTTGACGAAAGAGTCGCTGAAGCCTGCTCAGGCGCGTGCATTGGTGTACAGCA
CAATGAACGACAAACAGCTCGCTGAATTCGAGGGCACGCATGAGTGCAACTTTGCGATCAGCGCACCGGGTATCGGACGT
TTCCGTGTCAGTGCCTTCATCCAGCGTAATACGCCGGGTATGGTGTTGCGCCGAATCGACACCTATATTCCCAGCCTCGA
AGAGCTGAATCTACCGCCGGTGCTGAAAGATCTGTCGATGACCAAGCGAGGCTTGATCCTGTTTGTCGGTGGTACTTCAA
CCGGTAAATCGACCTCGCTGGCGTCGATGATCGACTATCGTAATGAAAACTATGCCGGTCATATCATCACCATTGAGGAT
CCGATCGAGTATATCCACAATCACAAGAAGAGCATCATCACTCAGCGTGAGGTGGGACTCGATACCGACTCCTTTGAAAT
TGCACTGAAGAATACGTTGCGTCAGGCGCCGGATGTGATTCTGATGGGTGAGATTCGCTCCATGGAAACGATGAAGTATG
GTCTGCAGTTTGCCGAGACCGGGCACCTGTGTATGGCAACGTTGCATGCCAACAACGCCAACCAGGCGCTGGATCGTATC
ATCTCCTTCTTCCCGGCGGATCATCATGATCAGTTGTGGATGGACCTGTCACTGAACCTGAAGGCGATAGTGGCGCAGCA
GCTGTTGCCGACTGTTGATGGCAAAGGACGACGTGCAGCGATTGAGGTGTTGATCAATACGCCACTGATCAAGGACCTGA
TCCGCAAGGCCGATGTGCATGAGATCAAGGATGTGATGAAAAAATCCACCAACCTTGGTATGCAGACCTTTGATCAGTCG
CTGTTCAAACTCTATAAAGAGGGTGTGATCGCTTACGATGTGGCGCTGACCCATGCTGACTCGGCCAACGATCTGCGTCT
GATGATCAAGCTCGATGGCTCCACAGCCGTCCCTGAAGGTAAGGGTGGTGAAGGCGATATGCCAAGCTTCACCTTGCAGG
ATGAGGAGGATAACCTGAATATTTCAGGCTCGGTTGACGTTAAGGCGATGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(80-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.014

91.026

0.628
  pilU Acinetobacter baylyi ADP1

62.674

92.051

0.577
  pilU Vibrio cholerae strain A1552

56.286

89.744

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.838

86.923

0.39
  pilT Acinetobacter baumannii strain A118

43.658

86.923

0.379
  pilT Acinetobacter nosocomialis M2

43.658

86.923

0.379
  pilT Acinetobacter baumannii D1279779

43.658

86.923

0.379
  pilT Pseudomonas aeruginosa PAK

42.985

85.897

0.369
  pilT Pseudomonas stutzeri DSM 10701

42.687

85.897

0.367
  pilT Acinetobacter baylyi ADP1

41.935

87.436

0.367
  pilT Neisseria meningitidis 8013

41.159

88.462

0.364
  pilT Neisseria gonorrhoeae MS11

40.87

88.462

0.362

Multiple sequence alignment