Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACKQF3_RS02395 Genome accession   NZ_CP175806
Coordinates   503897..504352 (-) Length   151 a.a.
NCBI ID   WP_162923374.1    Uniprot ID   -
Organism   Vibrio cholerae strain N19-2759     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498897..509352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKQF3_RS02375 (ACKQF3_02375) coaE 499402..500010 (-) 609 WP_000011549.1 dephospho-CoA kinase -
  ACKQF3_RS02380 (ACKQF3_02380) pilD 500007..500882 (-) 876 WP_000418750.1 A24 family peptidase Machinery gene
  ACKQF3_RS02385 (ACKQF3_02385) pilC 500940..502166 (-) 1227 WP_000648512.1 type II secretion system F family protein Machinery gene
  ACKQF3_RS02390 (ACKQF3_02390) pilB 502209..503897 (-) 1689 WP_317006818.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACKQF3_RS02395 (ACKQF3_02395) pilA 503897..504352 (-) 456 WP_162923374.1 pilin Machinery gene
  ACKQF3_RS02400 (ACKQF3_02400) nadC 504597..505487 (-) 891 WP_000665275.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACKQF3_RS02405 (ACKQF3_02405) ampD 505789..506334 (+) 546 WP_084988581.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACKQF3_RS02410 (ACKQF3_02410) fldB 506612..507130 (-) 519 WP_084988580.1 flavodoxin FldB -
  ACKQF3_RS02415 (ACKQF3_02415) xerD 507269..508177 (+) 909 WP_084988579.1 site-specific tyrosine recombinase XerD -
  ACKQF3_RS02420 (ACKQF3_02420) dsbC 508194..508946 (+) 753 WP_123162945.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15569.56 Da        Isoelectric Point: 8.4553

>NTDB_id=1078968 ACKQF3_RS02395 WP_162923374.1 503897..504352(-) (pilA) [Vibrio cholerae strain N19-2759]
MNAYKNKLQQGFTLIELMIVVAVIGVLAAIAVPQYQKYVAKSEAASALATLTGLKTNAEAYTVETGSFPTDTQQAQLGTP
SSAMGSIAYANSGNSGAAGTITFSFTGTVSPSLKNSAIRLKRDGSGNWSCESKTSLDSGVIPKGCNSGTFE

Nucleotide


Download         Length: 456 bp        

>NTDB_id=1078968 ACKQF3_RS02395 WP_162923374.1 503897..504352(-) (pilA) [Vibrio cholerae strain N19-2759]
ATGAACGCGTATAAAAACAAACTACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCTGTAATTGGGGTGCT
AGCAGCAATTGCGGTGCCTCAATACCAGAAATACGTAGCGAAAAGTGAAGCAGCTTCTGCATTAGCTACGTTAACTGGTT
TAAAAACCAATGCAGAGGCTTATACCGTTGAAACAGGATCTTTTCCCACAGATACTCAACAAGCACAACTAGGTACACCA
TCATCTGCAATGGGTTCCATCGCATATGCAAATTCAGGAAATAGTGGTGCAGCAGGAACAATAACATTTTCTTTTACTGG
TACAGTTAGCCCTAGTTTGAAAAACAGTGCTATCCGTTTAAAACGAGATGGCTCAGGAAACTGGTCATGTGAATCAAAAA
CTAGTTTAGATAGTGGAGTGATCCCTAAAGGATGTAATTCTGGCACTTTCGAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50

100

0.51
  pilA Vibrio cholerae strain A1552

50

100

0.51
  pilA Vibrio cholerae C6706

50

100

0.51
  pilA Pseudomonas aeruginosa PAK

46.358

100

0.464
  pilA Vibrio parahaemolyticus RIMD 2210633

46.212

87.417

0.404
  pilA Acinetobacter baumannii strain A118

39.333

99.338

0.391
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.31

96.026

0.377
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.161

94.702

0.371

Multiple sequence alignment