Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACKQF3_RS02390 Genome accession   NZ_CP175806
Coordinates   502209..503897 (-) Length   562 a.a.
NCBI ID   WP_317006818.1    Uniprot ID   -
Organism   Vibrio cholerae strain N19-2759     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 497209..508897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKQF3_RS02365 (ACKQF3_02365) yacG 498275..498472 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  ACKQF3_RS02370 (ACKQF3_02370) zapD 498630..499370 (-) 741 WP_000207194.1 cell division protein ZapD -
  ACKQF3_RS02375 (ACKQF3_02375) coaE 499402..500010 (-) 609 WP_000011549.1 dephospho-CoA kinase -
  ACKQF3_RS02380 (ACKQF3_02380) pilD 500007..500882 (-) 876 WP_000418750.1 A24 family peptidase Machinery gene
  ACKQF3_RS02385 (ACKQF3_02385) pilC 500940..502166 (-) 1227 WP_000648512.1 type II secretion system F family protein Machinery gene
  ACKQF3_RS02390 (ACKQF3_02390) pilB 502209..503897 (-) 1689 WP_317006818.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACKQF3_RS02395 (ACKQF3_02395) pilA 503897..504352 (-) 456 WP_162923374.1 pilin Machinery gene
  ACKQF3_RS02400 (ACKQF3_02400) nadC 504597..505487 (-) 891 WP_000665275.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACKQF3_RS02405 (ACKQF3_02405) ampD 505789..506334 (+) 546 WP_084988581.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACKQF3_RS02410 (ACKQF3_02410) fldB 506612..507130 (-) 519 WP_084988580.1 flavodoxin FldB -
  ACKQF3_RS02415 (ACKQF3_02415) xerD 507269..508177 (+) 909 WP_084988579.1 site-specific tyrosine recombinase XerD -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62071.18 Da        Isoelectric Point: 6.0856

>NTDB_id=1078967 ACKQF3_RS02390 WP_317006818.1 502209..503897(-) (pilB) [Vibrio cholerae strain N19-2759]
MLTNLIAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=1078967 ACKQF3_RS02390 WP_317006818.1 502209..503897(-) (pilB) [Vibrio cholerae strain N19-2759]
ATGCTCACCAACCTTATTGCTATCTTGCGTCAGGCGGAATTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGGACTTCGGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAAGAGCTGACCGAGCAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCCCTGTGTCAACAGCTTGGGCTGCGTGAA
CTGATAACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTACTGCTGCTCGCGGTCTCTGACCCAACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGGCTGCAAGTTGAACTGGCACTGGCCGATCACCGTGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCGGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTACTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGTGATGGTATTTTGGTGGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGTTTGCCGCAAGACGGGCGAATTAAACTGCGCCTAAGCCGCGATACCGCGAT
TGATATGCGCGTTTCAACACTCCCAACCTTATGGGGAGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTACCTCAACGCCCTCAAAAGACCACAAGGGATGATC
TTGATGACAGGCCCCACCGGCAGCGGCAAAACTGTTTCGCTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCAAAAATCGGCTTTG
GCTTTGCCGAGGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCAGAAATCGCGGTAAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCACACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCGAATATGGGGGTGGAGCCGTTTAACCTCGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCCTTTCAGCCCAATGAA
ATCTTGTATGAAGCGAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAGCGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.11

100

0.991
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

52.838

90.925

0.48
  pilB Acinetobacter baylyi ADP1

50.385

92.527

0.466
  pilB Acinetobacter baumannii D1279779

50.192

92.527

0.464
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37.086

100

0.399
  pilF Thermus thermophilus HB27

39.372

96.263

0.379
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37

Multiple sequence alignment