Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACJJWD_RS25230 Genome accession   NZ_CP174505
Coordinates   5537899..5538333 (-) Length   144 a.a.
NCBI ID   WP_407714666.1    Uniprot ID   -
Organism   Comamonas testosteroni strain VSPL.dLG     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5532899..5543333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJJWD_RS25205 (ACJJWD_25220) - 5532950..5533423 (+) 474 WP_043003320.1 hypothetical protein -
  ACJJWD_RS25210 (ACJJWD_25225) - 5533534..5533884 (+) 351 WP_029158475.1 phage holin family protein -
  ACJJWD_RS25215 (ACJJWD_25230) - 5533843..5535474 (-) 1632 WP_407716352.1 M48 family metalloprotease -
  ACJJWD_RS25220 (ACJJWD_25235) moaC 5535675..5536136 (+) 462 WP_407716353.1 cyclic pyranopterin monophosphate synthase MoaC -
  ACJJWD_RS25225 (ACJJWD_25240) - 5536146..5537732 (-) 1587 WP_407714665.1 Wzy polymerase domain-containing protein -
  ACJJWD_RS25230 (ACJJWD_25245) pilA 5537899..5538333 (-) 435 WP_407714666.1 pilin Machinery gene
  ACJJWD_RS25235 (ACJJWD_25250) - 5538523..5539242 (-) 720 WP_407714667.1 FHA domain-containing protein -
  ACJJWD_RS25240 (ACJJWD_25255) ssb 5539555..5540103 (-) 549 WP_407714668.1 single-stranded DNA-binding protein Machinery gene
  ACJJWD_RS25245 (ACJJWD_25260) - 5540340..5541512 (-) 1173 WP_407716354.1 MFS transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14742.04 Da        Isoelectric Point: 9.6049

>NTDB_id=1077459 ACJJWD_RS25230 WP_407714666.1 5537899..5538333(-) (pilA) [Comamonas testosteroni strain VSPL.dLG]
MKRSIQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVSEGLVQAAAAKTLVSENAATGRDLSLGFTGISATSNVAS
LAVASNNGVITVVTSSAAKNITLTLTPTVAGVALASGTVPDGNISWKCGISDTTKQKYVPSECR

Nucleotide


Download         Length: 435 bp        

>NTDB_id=1077459 ACJJWD_RS25230 WP_407714666.1 5537899..5538333(-) (pilA) [Comamonas testosteroni strain VSPL.dLG]
ATGAAGCGTTCCATTCAAAAGGGTTTCACCCTGATCGAACTGATGATCGTTGTGGCGATCATTGGTATTCTGGCTGCCGT
GGCTTTGCCTGCTTATCAGGATTACACCGTGCGCGCACGCGTGTCTGAAGGTTTGGTGCAAGCTGCTGCCGCTAAGACAC
TGGTTTCTGAAAACGCAGCTACTGGCCGTGACCTGAGCCTGGGGTTTACAGGAATTTCTGCGACCAGCAATGTAGCAAGC
TTGGCTGTGGCATCTAATAACGGTGTTATTACTGTTGTTACCTCCTCTGCGGCAAAGAACATTACGCTGACATTGACCCC
TACAGTTGCAGGGGTTGCACTTGCCTCTGGTACTGTGCCCGACGGCAACATCTCTTGGAAGTGCGGTATTTCTGACACTA
CAAAGCAGAAGTACGTTCCTTCTGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.235

100

0.576
  comP Acinetobacter baylyi ADP1

48.387

100

0.521
  pilA2 Legionella pneumophila str. Paris

50.694

100

0.507
  pilA2 Legionella pneumophila strain ERS1305867

50

100

0.5
  pilE Neisseria gonorrhoeae MS11

37.423

100

0.424
  pilE Neisseria gonorrhoeae strain FA1090

37.267

100

0.417
  pilA/pilA1 Eikenella corrodens VA1

35.294

100

0.417
  pilA Pseudomonas aeruginosa PAK

38.926

100

0.403
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.255

100

0.396
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.424

100

0.382
  pilA Acinetobacter baumannii strain A118

36.806

100

0.368

Multiple sequence alignment