Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   GEV1001_RS08125 Genome accession   NZ_CP173422
Coordinates   1891749..1892177 (-) Length   142 a.a.
NCBI ID   WP_011348339.1    Uniprot ID   Q3BQ79
Organism   Xanthomonas perforans strain GEV1001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1886749..1897177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GEV1001_RS08115 pilR 1888273..1889667 (+) 1395 WP_046931914.1 sigma-54 dependent transcriptional regulator Regulator
  GEV1001_RS08120 pilB 1889882..1891591 (-) 1710 WP_039416953.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GEV1001_RS08125 pilA 1891749..1892177 (-) 429 WP_011348339.1 pilin Machinery gene
  GEV1001_RS08130 pilC 1892519..1893781 (+) 1263 WP_011348340.1 type II secretion system F family protein Machinery gene
  GEV1001_RS08135 - 1893788..1894651 (+) 864 WP_008576868.1 A24 family peptidase -
  GEV1001_RS08140 coaE 1894665..1895276 (+) 612 WP_011348341.1 dephospho-CoA kinase -
  GEV1001_RS08145 - 1895458..1896498 (+) 1041 WP_011348342.1 RHS repeat domain-containing protein -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 14343.43 Da        Isoelectric Point: 9.1206

>NTDB_id=1072213 GEV1001_RS08125 WP_011348339.1 1891749..1892177(-) (pilA) [Xanthomonas perforans strain GEV1001]
MKKQNGFTLIELMIVVAIIAILAAIALPAYQDYTVRGRVSEAMVAASAAKTVVAENAANGSALNSGWTAPTATNNVASVA
VAAATGNITVTTTDKAGGGTIIFAPTANGAALTSGTVPTDRISWDCKGGTLVAKYRPAECRT

Nucleotide


Download         Length: 429 bp        

>NTDB_id=1072213 GEV1001_RS08125 WP_011348339.1 1891749..1892177(-) (pilA) [Xanthomonas perforans strain GEV1001]
ATGAAGAAGCAAAACGGCTTTACCCTTATCGAACTGATGATCGTTGTCGCGATCATCGCAATCCTTGCCGCCATCGCGTT
GCCGGCCTACCAGGACTACACCGTTCGCGGCCGCGTCTCTGAAGCGATGGTTGCCGCGTCTGCCGCCAAGACGGTGGTGG
CCGAAAATGCCGCCAACGGCTCTGCCCTGAATAGCGGGTGGACCGCTCCTACGGCGACCAATAATGTTGCCAGCGTAGCC
GTTGCTGCCGCTACGGGCAATATTACTGTGACCACTACCGATAAGGCCGGCGGCGGCACGATCATTTTTGCTCCCACCGC
TAACGGCGCTGCGCTGACTTCCGGCACCGTTCCTACCGATCGCATTTCGTGGGATTGCAAGGGTGGCACCTTGGTTGCCA
AGTACCGTCCGGCTGAATGCCGTACCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.572

100

0.577

  pilA2 Legionella pneumophila str. Paris

54.795

100

0.563

  pilA2 Legionella pneumophila strain ERS1305867

54.795

100

0.563

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.894

100

0.528

  comP Acinetobacter baylyi ADP1

47.468

100

0.528

  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.096

100

0.423

  pilA Acinetobacter baumannii strain A118

39.716

99.296

0.394

  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.571

98.592

0.38

  pilA Vibrio cholerae C6706

33.766

100

0.366

  pilA Vibrio cholerae strain A1552

33.766

100

0.366

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

33.766

100

0.366


Multiple sequence alignment