Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACFELE_RS25020 Genome accession   NZ_CP172856
Coordinates   5337211..5337663 (-) Length   150 a.a.
NCBI ID   WP_024928732.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238632E_ID011     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5332211..5342663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELE_RS25000 (ACFELE_25000) ampD 5332928..5333494 (-) 567 WP_003116245.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACFELE_RS25005 (ACFELE_25005) - 5333645..5335900 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  ACFELE_RS25010 (ACFELE_25010) nadC 5336186..5337034 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFELE_RS25020 (ACFELE_25020) pilA 5337211..5337663 (-) 453 WP_024928732.1 pilin Machinery gene
  ACFELE_RS25025 (ACFELE_25025) pilB 5337895..5339595 (+) 1701 WP_010793793.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFELE_RS25030 (ACFELE_25030) pilC 5339599..5340819 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  ACFELE_RS25035 (ACFELE_25035) pilD 5340823..5341695 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACFELE_RS25040 (ACFELE_25040) coaE 5341692..5342303 (+) 612 WP_010793792.1 dephospho-CoA kinase -
  ACFELE_RS25045 (ACFELE_25045) yacG 5342300..5342500 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15413.63 Da        Isoelectric Point: 8.4829

>NTDB_id=1069421 ACFELE_RS25020 WP_024928732.1 5337211..5337663(-) (pilA) [Pseudomonas aeruginosa strain 238632E_ID011]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGENIKIGSQASTADTTYV
GVDATANKLGTIAVTIGNNGAGTVTFTFAAGNSSPKNSGKIITLTRTDAGSWACTSTQDEMFIPKGCKAP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=1069421 ACFELE_RS25020 WP_024928732.1 5337211..5337663(-) (pilA) [Pseudomonas aeruginosa strain 238632E_ID011]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCACTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATCGCTGGGGAAAATATTAAAATTGGTTCCCAAGCATCTACTGCAGATACTACCTATGTA
GGTGTTGATGCTACCGCAAATAAGCTTGGCACTATTGCTGTGACAATTGGGAACAATGGCGCAGGTACCGTGACTTTCAC
TTTTGCAGCCGGCAATTCCAGTCCGAAAAACTCCGGTAAAATTATCACCCTGACCCGGACTGATGCAGGGTCGTGGGCTT
GTACTTCTACCCAGGACGAAATGTTCATCCCTAAAGGTTGCAAAGCGCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

68.456

99.333

0.68
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.333

100

0.473
  pilA Vibrio parahaemolyticus RIMD 2210633

46.939

98

0.46
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.671

100

0.46
  pilA Vibrio cholerae strain A1552

43.671

100

0.46
  pilA Vibrio cholerae C6706

43.671

100

0.46
  pilA Acinetobacter baumannii strain A118

44.295

99.333

0.44
  comP Acinetobacter baylyi ADP1

41.558

100

0.427
  pilA2 Legionella pneumophila strain ERS1305867

42.282

99.333

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.333

0.413
  pilA2 Legionella pneumophila str. Paris

41.611

99.333

0.413

Multiple sequence alignment