Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACFELE_RS25030 Genome accession   NZ_CP172856
Coordinates   5339599..5340819 (+) Length   406 a.a.
NCBI ID   WP_009315898.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238632E_ID011     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5334599..5345819
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELE_RS25010 (ACFELE_25010) nadC 5336186..5337034 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFELE_RS25020 (ACFELE_25020) pilA 5337211..5337663 (-) 453 WP_024928732.1 pilin Machinery gene
  ACFELE_RS25025 (ACFELE_25025) pilB 5337895..5339595 (+) 1701 WP_010793793.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFELE_RS25030 (ACFELE_25030) pilC 5339599..5340819 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  ACFELE_RS25035 (ACFELE_25035) pilD 5340823..5341695 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACFELE_RS25040 (ACFELE_25040) coaE 5341692..5342303 (+) 612 WP_010793792.1 dephospho-CoA kinase -
  ACFELE_RS25045 (ACFELE_25045) yacG 5342300..5342500 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ACFELE_RS25050 (ACFELE_25050) - 5342537..5342746 (-) 210 WP_003094660.1 hypothetical protein -
  ACFELE_RS25055 (ACFELE_25055) - 5342852..5343541 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  ACFELE_RS25060 (ACFELE_25060) - 5343538..5344008 (-) 471 WP_010793791.1 hypothetical protein -
  ACFELE_RS25065 (ACFELE_25065) - 5344005..5344430 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  ACFELE_RS25070 (ACFELE_25070) - 5344564..5345193 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  ACFELE_RS25075 (ACFELE_25075) - 5345190..5345639 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44288.04 Da        Isoelectric Point: 9.8784

>NTDB_id=1069423 ACFELE_RS25030 WP_009315898.1 5339599..5340819(+) (pilC) [Pseudomonas aeruginosa strain 238632E_ID011]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGAIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1069423 ACFELE_RS25030 WP_009315898.1 5339599..5340819(+) (pilC) [Pseudomonas aeruginosa strain 238632E_ID011]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGTTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCACAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGTGTGGGGGCGATTGGCTTTACCTTCAAG
GAACTGCATAAACGTTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCAGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baumannii D1279779

60.891

99.507

0.606
  pilC Acinetobacter baylyi ADP1

60.591

100

0.606
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.562

97.783

0.406
  pilC Thermus thermophilus HB27

37.157

98.768

0.367

Multiple sequence alignment