Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACGHX3_RS03505 Genome accession   NZ_CP171632
Coordinates   787131..788270 (-) Length   379 a.a.
NCBI ID   WP_162336981.1    Uniprot ID   -
Organism   Pseudoxanthomonas japonensis strain LY0623     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782131..793270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHX3_RS03465 (ACGHX3_03465) sugE 782325..782645 (+) 321 WP_394696409.1 quaternary ammonium compound efflux SMR transporter SugE -
  ACGHX3_RS03470 (ACGHX3_03470) coq7 782674..783315 (-) 642 WP_394696410.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  ACGHX3_RS03475 (ACGHX3_03475) rplM 783507..783935 (+) 429 WP_055936916.1 50S ribosomal protein L13 -
  ACGHX3_RS03480 (ACGHX3_03480) rpsI 783938..784330 (+) 393 WP_162110517.1 30S ribosomal protein S9 -
  ACGHX3_RS03490 (ACGHX3_03490) - 784507..785253 (+) 747 WP_394696411.1 2OG-Fe dioxygenase family protein -
  ACGHX3_RS03495 (ACGHX3_03495) - 785316..786305 (+) 990 WP_394696412.1 fumarylacetoacetate hydrolase family protein -
  ACGHX3_RS03500 (ACGHX3_03500) maiA 786403..787062 (+) 660 WP_394696413.1 maleylacetoacetate isomerase -
  ACGHX3_RS03505 (ACGHX3_03505) pilU 787131..788270 (-) 1140 WP_162336981.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGHX3_RS03510 (ACGHX3_03510) - 788364..788759 (+) 396 WP_313929674.1 DUF4398 domain-containing protein -
  ACGHX3_RS03515 (ACGHX3_03515) - 788756..789652 (+) 897 WP_394696414.1 OmpA family protein -
  ACGHX3_RS03520 (ACGHX3_03520) - 790010..790228 (+) 219 WP_055940917.1 YdcH family protein -
  ACGHX3_RS03525 (ACGHX3_03525) - 790340..791710 (+) 1371 WP_394696415.1 pyridoxal-phosphate dependent enzyme -
  ACGHX3_RS03530 (ACGHX3_03530) - 791810..793006 (+) 1197 WP_394696416.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 41903.21 Da        Isoelectric Point: 5.9760

>NTDB_id=1062407 ACGHX3_RS03505 WP_162336981.1 787131..788270(-) (pilU) [Pseudoxanthomonas japonensis strain LY0623]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPVFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSVIPTIEELNLPPVLKDIIMTPRGLVLIVGSTGSGKSTSLASMIDYRNTTSTGHILTIED
PIEYLHKHKQSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGTDGRRMPAAEVLINTPMIRDLLRRGQVHEIKAAMEESLEEGMETFDQCL
FRMVKEGQIEQEEALRAADSRDGLALKFRLSEGSKGEHDPYADYDSGGSSSPRITHGFG

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1062407 ACGHX3_RS03505 WP_162336981.1 787131..788270(-) (pilU) [Pseudoxanthomonas japonensis strain LY0623]
ATGGACATCGGCTATTTCCTGAAGCTGATGACCGAGAAGAACGCCTCGGACATGTTCCTCACGACCGGGGCGCCGGTCTA
CATCAAGATCGAGGGCAAGCTCTACCCGCTGGGCAATACCGGGCTGCCGCCCGGCATGGTCAAGAAGATCGCGTACTCGC
TGATGGACGAAGGCCAGGTGCCGGTGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCAGGACGCCGGCCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTCATCCGTGCCATCCGCAGCGTGATCCCGACCATCGA
GGAGCTGAACCTGCCGCCGGTGCTGAAGGACATCATCATGACCCCGCGCGGGCTGGTGCTGATCGTCGGTTCCACCGGTT
CGGGCAAGTCGACCTCGCTGGCGTCGATGATCGACTACCGCAACACCACCAGCACCGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAACAGTCGATCGTCAACCAGCGCGAAGTCGGCCTGGATACGCACGCCTTCCACAA
CGCACTGAAGAACGCGATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAGATCCTGGACGCCACCACCATGGAAGCCG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGTTGCACTCGAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTCTCCCAGCG
CCTGGTGAAGGGCACCGACGGGCGCCGCATGCCGGCGGCGGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GGCGCGGACAGGTGCACGAGATCAAGGCCGCGATGGAGGAGTCGCTGGAAGAAGGCATGGAGACCTTCGACCAGTGCCTG
TTCCGCATGGTCAAGGAAGGCCAGATCGAGCAGGAGGAAGCGCTGCGCGCCGCCGACTCGCGCGACGGCCTTGCGCTCAA
GTTCCGCCTGTCCGAAGGCAGCAAGGGCGAGCACGATCCCTACGCGGACTACGACAGCGGCGGCAGCAGTTCGCCGCGCA
TCACCCACGGCTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

94.723

0.496
  pilU Vibrio cholerae strain A1552

47.2

98.945

0.467
  pilU Acinetobacter baylyi ADP1

49.153

93.404

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.56

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.308

89.182

0.377
  pilT Acinetobacter baumannii strain A118

41.42

89.182

0.369
  pilT Acinetobacter nosocomialis M2

41.42

89.182

0.369
  pilT Acinetobacter baumannii D1279779

41.42

89.182

0.369
  pilT Acinetobacter baylyi ADP1

41.176

89.71

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.182

0.367
  pilT Legionella pneumophila strain Lp02

40.533

89.182

0.361
  pilT Legionella pneumophila strain ERS1305867

40.533

89.182

0.361

Multiple sequence alignment