Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACGK9W_RS19880 Genome accession   NZ_CP171359
Coordinates   4264143..4265264 (+) Length   373 a.a.
NCBI ID   WP_405418343.1    Uniprot ID   -
Organism   Marinobacter flavimaris strain ZYH30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4259143..4270264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGK9W_RS19860 (ACGK9W_19860) - 4260703..4261287 (-) 585 WP_008174183.1 YggT family protein -
  ACGK9W_RS19865 (ACGK9W_19865) proC 4261310..4262143 (-) 834 WP_014575886.1 pyrroline-5-carboxylate reductase -
  ACGK9W_RS19870 (ACGK9W_19870) - 4262205..4262909 (-) 705 WP_405418340.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACGK9W_RS19875 (ACGK9W_19875) pilT 4263050..4264084 (+) 1035 WP_008174189.1 type IV pilus twitching motility protein PilT Machinery gene
  ACGK9W_RS19880 (ACGK9W_19880) pilU 4264143..4265264 (+) 1122 WP_405418343.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACGK9W_RS19885 (ACGK9W_19885) - 4265504..4266850 (+) 1347 WP_405418346.1 OmpP1/FadL family transporter -
  ACGK9W_RS19890 (ACGK9W_19890) - 4266871..4267245 (-) 375 WP_273046231.1 hypothetical protein -
  ACGK9W_RS19895 (ACGK9W_19895) polA 4267478..4270210 (-) 2733 WP_405418350.1 DNA polymerase I -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41486.62 Da        Isoelectric Point: 6.5037

>NTDB_id=1060876 ACGK9W_RS19880 WP_405418343.1 4264143..4265264(+) (pilU) [Marinobacter flavimaris strain ZYH30]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTREFVYGAMNDKQRAEFEETHECNFAISARGIGR
FRVSAFFQRNLCGMVLRRIEVKIPQIDDLSLPEVIKELAMTKRGLIMFVGATGTGKSTSLAAMLGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHNQIWMDLSLNLKAIVAQQLVPTPDGKGRKAVIEVLINTPLVSDLIRKGEVHRLKELMSKSNESGMQTFDQA
LYQLYAEGSITYEDALAHADSANDLRLMIKLGADAQGADKLSSSVDKLSIQDD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1060876 ACGK9W_RS19880 WP_405418343.1 4264143..4265264(+) (pilU) [Marinobacter flavimaris strain ZYH30]
ATGGAATTCGAAAAACTGTTACGGCTGATGGTGGAGAAGGGCGGTTCGGACCTGTTTATTACAGCGGGTGTGCCGCCGAG
TATGAAGGTGAACGGGAAGGTTCTACCGGTCACCAAGAACGCGTTGACGCCGGAGCAGACCAGGGAGTTCGTTTACGGCG
CCATGAACGACAAGCAGCGCGCCGAATTCGAGGAAACCCATGAGTGTAACTTCGCTATCAGTGCCCGTGGCATTGGCCGT
TTCCGTGTTTCTGCCTTTTTCCAGCGCAACCTGTGCGGGATGGTGCTGCGCCGGATCGAGGTAAAGATCCCGCAAATCGA
CGATCTGTCGCTGCCGGAAGTGATCAAGGAACTGGCCATGACCAAGCGTGGCCTGATCATGTTCGTGGGCGCCACCGGCA
CCGGTAAGTCTACGTCGCTGGCGGCGATGCTGGGGCACCGGAACCGCAACAGCCGTGGCCACATCATTTCCATTGAAGAC
CCGATCGAATTCGTACACCAGCACCAGGGCTGTATCGTTACCCAGCGTGAAGTGGGTATCGACACCGACAGCTTCGAAGT
AGCGCTGAAGAACACGCTGCGTCAGGCGCCCGACGTGATCCTGATCGGTGAGGTGCGTACCCGGCAGACCATGGAATACT
CCGTGCAATTCGCCGAGACCGGTCACCTGTGTCTGGCCACCCTGCACGCCAACAACGCGAACCAGGCGCTGGACCGGATT
ATCCAGTTCTTCCCTCCGGAACAGCATAACCAGATCTGGATGGACCTCTCCCTGAACCTCAAGGCCATCGTGGCCCAGCA
GCTGGTGCCAACGCCGGACGGCAAGGGCCGGAAGGCGGTTATCGAGGTGCTGATCAATACCCCGCTGGTATCGGACCTCA
TCCGGAAGGGCGAGGTGCATCGGCTGAAAGAGCTGATGTCCAAATCCAACGAATCCGGCATGCAGACCTTTGACCAGGCG
CTTTATCAGCTCTATGCAGAAGGGTCGATTACTTATGAGGACGCCCTGGCTCATGCTGATTCTGCTAACGACCTGCGCCT
GATGATCAAACTGGGTGCCGACGCCCAGGGAGCCGACAAGCTCTCGTCTTCCGTCGACAAGCTTTCCATTCAGGATGACT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

79.515

99.464

0.791
  pilU Acinetobacter baylyi ADP1

65.169

95.442

0.622
  pilU Vibrio cholerae strain A1552

57.027

99.196

0.566
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

90.885

0.386
  pilT Pseudomonas aeruginosa PAK

40.896

89.812

0.367
  pilT Acinetobacter baumannii strain A118

40.597

89.812

0.365
  pilT Acinetobacter baumannii D1279779

40.597

89.812

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.597

89.812

0.365
  pilT Acinetobacter nosocomialis M2

40.299

89.812

0.362
  pilT Legionella pneumophila strain Lp02

40.299

89.812

0.362
  pilT Legionella pneumophila strain ERS1305867

40.299

89.812

0.362

Multiple sequence alignment