Detailed information NTG1060556

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACF6ZU_RS26870
• Genome accession: NZ_CP171224
• Coordinates: 5837510..5838619 (+)
• Length: 369 a.a.
• NCBI ID: WP_392888592.1
• Uniprot ID: -
• Organism: Pseudomonas migulae strain HY-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5832510..5843619

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF6ZU_RS26855 (ACF6ZU_26855)	-	5833464..5834396 (+)	933	WP_392888586.1	sugar kinase	-
ACF6ZU_RS26860 (ACF6ZU_26860)	-	5834511..5834786 (-)	276	WP_007898282.1	peptidylprolyl isomerase	-
ACF6ZU_RS26865 (ACF6ZU_26865)	-	5834853..5837390 (-)	2538	WP_392888590.1	PAS domain-containing protein	-
ACF6ZU_RS26870 (ACF6ZU_26870)	pilU	5837510..5838619 (+)	1110	WP_392888592.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACF6ZU_RS26875 (ACF6ZU_26875)	-	5838782..5840920 (+)	2139	WP_392888595.1	FUSC family protein	-
ACF6ZU_RS26880 (ACF6ZU_26880)	-	5840910..5841110 (+)	201	WP_392888597.1	DUF1656 domain-containing protein	-
ACF6ZU_RS26885 (ACF6ZU_26885)	-	5841115..5842071 (+)	957	WP_392888598.1	biotin/lipoyl-binding protein	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41148.18 Da
• Isoelectric Point: 6.3914

>NTDB_id=1060556 ACF6ZU_RS26870 WP_392888592.1 5837510..5838619(+) (pilU) [Pseudomonas migulae strain HY-2]
MEIDALLTLLASQNGSDLYLSTGAPPCARFDGVLKPLSDQPFKPAEIAAMAASIMDAEQRLEFDRELEMNLAISLPGVGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPPVLLESVMLKQGLILFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INLFPEDRRLQLLHDLANNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIGDLIRRNELDELKGIMEKSVDAGMQTFDSAL
YALVVEGAIDEDEALKHADSVNNLRLRLKLHAETSPAPHTPPRDWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=1060556 ACF6ZU_RS26870 WP_392888592.1 5837510..5838619(+) (pilU) [Pseudomonas migulae strain HY-2]
ATGGAAATCGATGCGCTGTTAACGCTGCTGGCCAGCCAGAATGGATCCGATCTGTACCTCTCCACCGGCGCGCCGCCCTG
TGCCCGGTTCGATGGAGTGTTAAAGCCCTTGAGCGATCAGCCGTTCAAACCGGCAGAAATTGCGGCGATGGCCGCGTCCA
TCATGGACGCCGAACAGCGGTTGGAGTTCGACCGGGAGCTGGAAATGAACCTGGCGATTTCGTTGCCGGGTGTCGGTCGT
TTCCGGGTCAATATTTTCAAGCAGCGCAACGATGTGTCGATCGTGGCGCGCAACATCAAACTCGACATCCCGCGCTTCGA
GGACCTCAAGCTGCCGCCGGTGCTGTTGGAGTCGGTCATGCTCAAGCAAGGGCTGATCCTGTTCGTCGGCGCTACCGATT
CGGGCAAGTCGACTTCACTGGCGGCGTTGATCGATTACCGAAACCGCCACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTCGAGTACATCCATCGCCACAAGAAATCGATCATCAATCAGCGCGAAGTTGGCGTGGACACCCGCAGTTTTCATGC
GGCGTTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGAGAGATCCGCGACCGCGAAACCATGGAGCACG
CGCTGGCGTTTGCCGACACCGGGCACCTGGTGATTTCCACGTTGCATGCCCACAACGCCCATCAGGCGCTGGACCGCATC
ATCAACCTCTTCCCGGAAGACAGGCGCCTGCAACTGCTGCATGACCTGGCCAACAACCTGAAAGCGTTCGTGTCCCAGCG
CTTGGTACGCACCCGAGACGGCCAACGCCGGGCGGCGGTGGAAGTGATGCTGGGAACCCCAACTATCGGCGACCTGATCC
GCCGTAACGAGCTGGATGAACTGAAAGGGATCATGGAGAAGTCCGTGGACGCGGGGATGCAGACGTTCGACAGCGCGCTG
TACGCCCTGGTCGTGGAAGGTGCGATCGATGAGGACGAGGCGCTGAAGCATGCGGACTCGGTGAACAATTTACGCTTGCG
ACTCAAGTTGCACGCCGAAACATCCCCAGCTCCCCACACCCCTCCGCGCGATTGGGGCCTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.901	96.206	0.547
pilU	Acinetobacter baylyi ADP1	52.661	96.748	0.509
pilU	Vibrio cholerae strain A1552	51.429	94.851	0.488
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.742	90.244	0.377
pilT	Vibrio cholerae strain A1552	41.742	90.244	0.377
pilT	Pseudomonas stutzeri DSM 10701	40.407	93.225	0.377
pilT	Pseudomonas aeruginosa PAK	39.826	93.225	0.371
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.528	91.87	0.363
pilT	Neisseria gonorrhoeae MS11	38.329	94.038	0.36
pilT	Neisseria meningitidis 8013	38.551	93.496	0.36
pilT	Legionella pneumophila strain Lp02	39.82	90.515	0.36
pilT	Legionella pneumophila strain ERS1305867	39.82	90.515	0.36