Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFJIT_RS13810 Genome accession   NZ_CP170559
Coordinates   3125907..3127043 (+) Length   378 a.a.
NCBI ID   WP_379653932.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. UC19_8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3102343..3128824 3125907..3127043 within 0


Gene organization within MGE regions


Location: 3102343..3128824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIT_RS13700 (ACFJIT_13700) - 3102343..3103326 (+) 984 WP_379653915.1 YbhN family protein -
  ACFJIT_RS13705 (ACFJIT_13705) - 3103449..3104837 (+) 1389 WP_379655779.1 UbiA family prenyltransferase -
  ACFJIT_RS13710 (ACFJIT_13710) - 3104837..3106144 (+) 1308 WP_379653916.1 FAD-dependent oxidoreductase -
  ACFJIT_RS13715 (ACFJIT_13715) - 3106147..3106875 (+) 729 WP_379653917.1 SDR family oxidoreductase -
  ACFJIT_RS13720 (ACFJIT_13720) - 3106877..3107821 (+) 945 WP_379653918.1 NAD-dependent epimerase/dehydratase family protein -
  ACFJIT_RS13725 (ACFJIT_13725) - 3107844..3109139 (+) 1296 WP_379653919.1 NAD(P)/FAD-dependent oxidoreductase -
  ACFJIT_RS13730 (ACFJIT_13730) - 3109136..3109528 (+) 393 WP_379653920.1 GtrA family protein -
  ACFJIT_RS13735 (ACFJIT_13735) - 3109603..3111078 (+) 1476 WP_379653921.1 hypothetical protein -
  ACFJIT_RS13740 (ACFJIT_13740) - 3111321..3111848 (-) 528 WP_379653922.1 hypothetical protein -
  ACFJIT_RS13745 (ACFJIT_13745) - 3111872..3113473 (-) 1602 WP_379653923.1 hypothetical protein -
  ACFJIT_RS13750 (ACFJIT_13750) - 3113551..3114525 (-) 975 WP_379653924.1 glycosyltransferase -
  ACFJIT_RS13755 (ACFJIT_13755) - 3114522..3115142 (-) 621 WP_379653925.1 glycosyltransferase -
  ACFJIT_RS13760 (ACFJIT_13760) - 3116113..3117045 (-) 933 Protein_2695 glycosyltransferase -
  ACFJIT_RS13765 (ACFJIT_13765) - 3117102..3118202 (-) 1101 WP_379653926.1 class I SAM-dependent methyltransferase -
  ACFJIT_RS13770 (ACFJIT_13770) - 3118264..3118824 (-) 561 WP_379653927.1 CatB-related O-acetyltransferase -
  ACFJIT_RS13775 (ACFJIT_13775) - 3118824..3120158 (-) 1335 WP_379653928.1 ABC transporter ATP-binding protein -
  ACFJIT_RS13780 (ACFJIT_13780) - 3120201..3121019 (-) 819 WP_379653929.1 ABC transporter permease -
  ACFJIT_RS13785 (ACFJIT_13785) - 3121046..3122245 (-) 1200 WP_379653930.1 cystathionine gamma-synthase -
  ACFJIT_RS13790 (ACFJIT_13790) - 3122341..3123711 (-) 1371 WP_130523082.1 pyridoxal-phosphate dependent enzyme -
  ACFJIT_RS13795 (ACFJIT_13795) - 3123899..3124117 (-) 219 WP_130515246.1 YdcH family protein -
  ACFJIT_RS13800 (ACFJIT_13800) - 3124452..3125342 (-) 891 WP_379653931.1 hypothetical protein -
  ACFJIT_RS13805 (ACFJIT_13805) - 3125339..3125764 (-) 426 WP_328590467.1 DUF4398 domain-containing protein -
  ACFJIT_RS13810 (ACFJIT_13810) pilU 3125907..3127043 (+) 1137 WP_379653932.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJIT_RS13815 (ACFJIT_13815) maiA 3127237..3127914 (-) 678 WP_130515237.1 maleylacetoacetate isomerase -
  ACFJIT_RS13820 (ACFJIT_13820) - 3128052..3128824 (+) 773 WP_379653933.1 IS5 family transposase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41798.04 Da        Isoelectric Point: 6.1390

>NTDB_id=1058027 ACFJIT_RS13810 WP_379653932.1 3125907..3127043(+) (pilU) [Pseudoxanthomonas sp. UC19_8]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAVALAEAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELRLPEVLKDIIMTPRGLVLLVGSTGSGKSTSLASMIDYRNSSTTGHILTIED
PIEYLHKHKQSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPETAHKNVLMNLALNLRAVVSQRLVKGVDGRRLPAAEVLINTPVIRDLLRRGQIHEIKQAMEESLEDGMESFDQCL
FRMAKEGRISQEEALRAADSRDGLALKFRLSEGASGEHDPYADLYDSSAPSTITHGFG

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1058027 ACFJIT_RS13810 WP_379653932.1 3125907..3127043(+) (pilU) [Pseudoxanthomonas sp. UC19_8]
ATGGACATCGGCTATTTCCTCAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACCACCGGCGCGCCCGTCTA
CATCAAGATCGAAGGCAGGCTCTACCCCCTTGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAAATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAGCTCAACATGGCCGTGGCCCTGGCCGAGGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTCATCCGCGCCATCCGCAGCAAGATCCCCTCGATCGA
GGAGCTGCGGCTGCCGGAGGTGCTCAAGGACATCATCATGACCCCGCGTGGGCTGGTGCTGCTGGTCGGCTCGACCGGCT
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGACTATCGCAACTCCAGCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGCAGTCCATCGTCAACCAGCGTGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCCATGCGCGAGGCGCCGGACGTGATTCTGATCGGCGAGATCCTGGATGCGACCACCATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGACCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTGTCCCAGCG
CCTGGTCAAGGGCGTGGACGGGCGCCGGCTGCCGGCCGCCGAGGTCCTGATCAACACCCCGGTAATCCGCGACCTGCTGC
GCCGCGGCCAGATCCACGAGATCAAGCAGGCCATGGAGGAGTCGCTGGAGGATGGCATGGAGTCCTTCGACCAGTGCCTG
TTCCGCATGGCCAAGGAAGGCAGGATCAGCCAGGAGGAGGCGCTGCGCGCGGCCGATTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGCCTCGGGCGAGCACGACCCTTACGCCGATCTGTACGACAGCAGCGCCCCCTCGACCATCA
CGCACGGCTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.696

94.974

0.481
  pilU Acinetobacter baylyi ADP1

49.3

94.444

0.466
  pilU Vibrio cholerae strain A1552

48.023

93.651

0.45
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.67

100

0.431
  pilT Pseudomonas aeruginosa PAK

41.716

89.418

0.373
  pilT Legionella pneumophila strain ERS1305867

41.42

89.418

0.37
  pilT Legionella pneumophila strain Lp02

41.42

89.418

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.418

0.368
  pilT Acinetobacter baumannii D1279779

40.828

89.418

0.365
  pilT Acinetobacter nosocomialis M2

40.828

89.418

0.365
  pilT Acinetobacter baumannii strain A118

40.828

89.418

0.365
  pilT Acinetobacter baylyi ADP1

40.294

89.947

0.362

Multiple sequence alignment