Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LMH68_RS09345 Genome accession   NZ_CP170175
Coordinates   2043301..2045019 (+) Length   572 a.a.
NCBI ID   WP_032545762.1    Uniprot ID   A0A4U2A109
Organism   Vibrio sp. F13 strain 10N.222.55.B3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2038301..2050019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH68_RS09325 (LMH68_009325) pilM 2040530..2041555 (+) 1026 WP_017061130.1 type IV pilus assembly protein PilM Machinery gene
  LMH68_RS09330 (LMH68_009330) pilN 2041539..2042138 (+) 600 WP_017061129.1 PilN domain-containing protein Machinery gene
  LMH68_RS09335 (LMH68_009335) pilO 2042131..2042760 (+) 630 WP_017061128.1 type 4a pilus biogenesis protein PilO Machinery gene
  LMH68_RS09340 (LMH68_009340) pilP 2042747..2043265 (+) 519 WP_017063694.1 pilus assembly protein PilP Machinery gene
  LMH68_RS09345 (LMH68_009345) pilQ 2043301..2045019 (+) 1719 WP_032545762.1 type IV pilus secretin PilQ Machinery gene
  LMH68_RS09350 (LMH68_009350) aroK 2045196..2045714 (+) 519 WP_017061125.1 shikimate kinase AroK -
  LMH68_RS09355 (LMH68_009355) aroB 2045740..2046828 (+) 1089 WP_017061124.1 3-dehydroquinate synthase -
  LMH68_RS09360 (LMH68_009360) - 2046908..2048425 (+) 1518 WP_017068836.1 AAA family ATPase -
  LMH68_RS09365 (LMH68_009365) - 2048495..2049343 (+) 849 WP_017061122.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62033.75 Da        Isoelectric Point: 4.3871

>NTDB_id=1055444 LMH68_RS09345 WP_032545762.1 2043301..2045019(+) (pilQ) [Vibrio sp. F13 strain 10N.222.55.B3]
MIKGISGLVSKALQGFAVSVMLVVSVLSHAESLPNKLENIDFRVNKDKDAVIIIELATSTAVVDVQRAQEGLSIELINTQ
VDDDKLYLLDVKDFATLVEGIEVYKETPSTRLLATISNDYQYEYNLKGRFIEVVISKPVLDEATKEKSVLEKEGKLISIN
FQDIPVRNVLQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEK
SRLEEELGELKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQ
IEARIVTVTEGNLDELGVRWGVSSTNGSFTVGGSIEGNHPSAITPYDDDSGSSVIDDYLNVNLGATSPNASSIAFQVAKL
GSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLD
LSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSE
LLIFVTPKVVIQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=1055444 LMH68_RS09345 WP_032545762.1 2043301..2045019(+) (pilQ) [Vibrio sp. F13 strain 10N.222.55.B3]
ATGATTAAAGGAATAAGTGGATTAGTGAGCAAGGCTCTACAAGGTTTTGCTGTGTCTGTGATGTTGGTTGTTAGCGTGCT
CAGCCATGCCGAGAGCTTACCGAACAAATTGGAGAATATTGATTTTAGGGTCAATAAGGATAAAGACGCCGTCATCATTA
TTGAGCTGGCGACCAGCACAGCAGTGGTGGATGTTCAACGAGCTCAGGAAGGGTTAAGTATCGAGCTAATCAATACTCAA
GTTGATGATGACAAGCTCTACCTGCTGGATGTGAAGGATTTCGCTACCTTGGTTGAAGGCATTGAAGTCTATAAAGAGAC
ACCAAGCACTCGGCTTCTAGCGACGATTAGCAATGACTATCAGTATGAATACAACCTAAAGGGGCGCTTTATTGAGGTCG
TGATCAGCAAACCGGTGCTCGATGAAGCGACCAAAGAAAAAAGCGTTCTAGAGAAAGAGGGCAAGCTGATATCGATTAAC
TTCCAGGATATCCCGGTACGAAATGTCCTGCAACTGATTGCCGATTACAACGACTTCAATCTCGTGGTGTCTGATTCGGT
AGCCGGAAACCTGACGTTACGCTTAGATGGCGTACCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAAGGCTTGG
ATAAGCGTGTTGATGGCAATGTTATCTTGGTCGCGCCGAAAGCGGAGCTCGACCTTCGAGAGCAACAAGCGTTAGAGAAA
TCGCGCTTAGAAGAGGAGTTAGGGGAGCTTAAATCGGAAATCATCAAGATTAACTTTGCTAAAGCCACCGACATCGCTGA
CATGATTGGAGGTGAAGGTGCGGTCAGCATGTTGTCCGATCGTGGTTCGATTACCATTGATGAGCGCACCAACTCACTGC
TCATTCGTGAATTGGAAGAAAACATTGCCGTGATCCGAGGCATCATTGAATCGTTAGACATTCCCGTGAAGCAGGTACAG
ATAGAGGCGCGTATTGTCACGGTTACTGAGGGTAATCTTGATGAGCTTGGCGTGCGCTGGGGCGTTTCCTCAACCAACGG
AAGCTTCACCGTTGGTGGCTCAATCGAAGGTAACCACCCATCAGCGATTACGCCGTATGACGACGATAGCGGCAGCAGCG
TGATTGATGACTATCTCAACGTCAATTTAGGGGCAACATCGCCGAACGCCTCGAGTATTGCGTTTCAGGTGGCCAAACTG
GGTTCAGATACCTTACTCGATCTTGAACTGTCGGCACTGCAACAAGAGTCAAAAGCTGAAATCATTTCTAGTCCACGCTT
AATCACCACCAATAAAAAGCCGGCTTATATTGAGCAAGGTACTGAAATTCCTTACTTAGAGTCATCTTCAAGTGGCGCTA
CGTCGGTTGCATTTAAGAAAGCCGTGTTGAGTCTTAAGGTGACACCGCAGATCACACCCGACAATCGTTTGGTATTGGAT
TTGAGCGTGACTCAAGATAGGCCAGGGCAAGTGGTAAAAACTGGAACCGGTGAAGCGGTCGCCATTGATACGCAAAGAAT
AGGTACGCAAGTGCTTGTTAATAATGGTGAAACTGTCGTGCTTGGCGGGATATTTCAGCACAGCGTGAGCAGTACAGTGG
ATAAAGTTCCTCTGTTGGGGGACCTGCCAGTTTTGGGCGCATTGTTCCGTCGCAGCTATGAAAATGTGGGTAAAAGTGAA
CTACTTATTTTTGTCACACCCAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(412-570)

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4U2A109

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

77.526

100

0.778
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.526

100

0.778
  pilQ Vibrio campbellii strain DS40M4

81.066

95.105

0.771
  pilQ Legionella pneumophila strain ERS1305867

42.491

95.455

0.406
  pilQ Legionella pneumophila strain Lp02

42.491

95.455

0.406
  pilQ Pseudomonas aeruginosa PAK

40.754

97.378

0.397
  comQ Acinetobacter baylyi ADP1

38.957

100

0.392
  pilQ Acinetobacter baumannii D1279779

37.651

100

0.381
  pilQ Acinetobacter baumannii strain A118

37.651

100

0.381

Multiple sequence alignment