Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   LMH68_RS09330 Genome accession   NZ_CP170175
Coordinates   2041539..2042138 (+) Length   199 a.a.
NCBI ID   WP_017061129.1    Uniprot ID   A0A4V5RQ38
Organism   Vibrio sp. F13 strain 10N.222.55.B3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2036539..2047138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH68_RS09310 (LMH68_009310) - 2036635..2036814 (-) 180 WP_229647423.1 hypothetical protein -
  LMH68_RS09315 (LMH68_009315) oxyR 2036813..2037706 (+) 894 WP_017061132.1 DNA-binding transcriptional regulator OxyR -
  LMH68_RS09320 (LMH68_009320) - 2037817..2040378 (-) 2562 WP_229647424.1 penicillin-binding protein 1A -
  LMH68_RS09325 (LMH68_009325) pilM 2040530..2041555 (+) 1026 WP_017061130.1 type IV pilus assembly protein PilM Machinery gene
  LMH68_RS09330 (LMH68_009330) pilN 2041539..2042138 (+) 600 WP_017061129.1 PilN domain-containing protein Machinery gene
  LMH68_RS09335 (LMH68_009335) pilO 2042131..2042760 (+) 630 WP_017061128.1 type 4a pilus biogenesis protein PilO Machinery gene
  LMH68_RS09340 (LMH68_009340) pilP 2042747..2043265 (+) 519 WP_017063694.1 pilus assembly protein PilP Machinery gene
  LMH68_RS09345 (LMH68_009345) pilQ 2043301..2045019 (+) 1719 WP_032545762.1 type IV pilus secretin PilQ Machinery gene
  LMH68_RS09350 (LMH68_009350) aroK 2045196..2045714 (+) 519 WP_017061125.1 shikimate kinase AroK -
  LMH68_RS09355 (LMH68_009355) aroB 2045740..2046828 (+) 1089 WP_017061124.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22971.43 Da        Isoelectric Point: 9.6562

>NTDB_id=1055441 LMH68_RS09330 WP_017061129.1 2041539..2042138(+) (pilN) [Vibrio sp. F13 strain 10N.222.55.B3]
MLHQINLLPWRDEIRAQHKKRFIHLVILGVIIALIGQWAVGHYFHDQQAKQQARLNYLNQYIAELDRQIQSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKGVEMHSIV
HNRKRFNKEFQTFKVSFVFSPVSLENTTASRKDKGAHHG

Nucleotide


Download         Length: 600 bp        

>NTDB_id=1055441 LMH68_RS09330 WP_017061129.1 2041539..2042138(+) (pilN) [Vibrio sp. F13 strain 10N.222.55.B3]
ATGTTGCATCAAATTAACCTGCTGCCGTGGCGTGATGAGATTCGTGCTCAGCACAAGAAGCGTTTTATCCATCTGGTCAT
TCTTGGCGTCATCATTGCGCTTATAGGGCAATGGGCGGTTGGTCACTATTTTCACGATCAACAAGCCAAGCAGCAAGCTC
GCCTCAATTACCTCAATCAGTACATTGCTGAGCTCGACCGACAAATTCAGTCGTTAAAAGTCGCAGAGCAAGAACACAAA
GCCATTCTGACTCGACTTGATGTGGTTGAGTCGCTGCAGCTTGGCCGTAATAAGACCACCGACTTCATGAACTTGATGCC
GGAGCTGATTCCAGAGGGTGTCTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATCAGCGACA
GCACTGCTCGTCTCGCCACCATGTTGGACAACCTCGAACGTTCAGATTCTTTGAAAGGGGTCGAGATGCACTCTATCGTT
CATAACCGCAAGCGCTTCAATAAAGAGTTTCAGACCTTCAAGGTCTCTTTTGTGTTTAGCCCTGTGTCTTTAGAAAACAC
AACCGCGAGCAGAAAAGATAAGGGGGCGCATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4V5RQ38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

63.5

100

0.638
  pilN Vibrio campbellii strain DS40M4

63.441

93.467

0.593

Multiple sequence alignment