Detailed information NTG1055440

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: LMH68_RS09325
• Genome accession: NZ_CP170175
• Coordinates: 2040530..2041555 (+)
• Length: 341 a.a.
• NCBI ID: WP_017061130.1
• Uniprot ID: A0A4U2B7T4
• Organism: Vibrio sp. F13 strain 10N.222.55.B3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2035530..2046555

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LMH68_RS09305 (LMH68_009305)	-	2035862..2036590 (-)	729	WP_017061133.1	glutathione peroxidase	-
LMH68_RS09310 (LMH68_009310)	-	2036635..2036814 (-)	180	WP_229647423.1	hypothetical protein	-
LMH68_RS09315 (LMH68_009315)	oxyR	2036813..2037706 (+)	894	WP_017061132.1	DNA-binding transcriptional regulator OxyR	-
LMH68_RS09320 (LMH68_009320)	-	2037817..2040378 (-)	2562	WP_229647424.1	penicillin-binding protein 1A	-
LMH68_RS09325 (LMH68_009325)	pilM	2040530..2041555 (+)	1026	WP_017061130.1	type IV pilus assembly protein PilM	Machinery gene
LMH68_RS09330 (LMH68_009330)	pilN	2041539..2042138 (+)	600	WP_017061129.1	PilN domain-containing protein	Machinery gene
LMH68_RS09335 (LMH68_009335)	pilO	2042131..2042760 (+)	630	WP_017061128.1	type 4a pilus biogenesis protein PilO	Machinery gene
LMH68_RS09340 (LMH68_009340)	pilP	2042747..2043265 (+)	519	WP_017063694.1	pilus assembly protein PilP	Machinery gene
LMH68_RS09345 (LMH68_009345)	pilQ	2043301..2045019 (+)	1719	WP_032545762.1	type IV pilus secretin PilQ	Machinery gene
LMH68_RS09350 (LMH68_009350)	aroK	2045196..2045714 (+)	519	WP_017061125.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38253.68 Da
• Isoelectric Point: 6.4182

>NTDB_id=1055440 LMH68_RS09325 WP_017061130.1 2040530..2041555(+) (pilM) [Vibrio sp. F13 strain 10N.222.55.B3]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQLASRLYPDKKNWLLVDVGIDQTSLGVIPQNSAPFYKDIAFGTQDLRSSDDPSDIESVFGTMEETH
QFIVNLIEKLKRQLQLYSSGNAQQPISGIWLMGEGASIPMVTEELERHFQLSCESLNPLSLFENKVAKRRRLPIDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=1055440 LMH68_RS09325 WP_017061130.1 2040530..2041555(+) (pilM) [Vibrio sp. F13 strain 10N.222.55.B3]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGACTGCCTTTTGGCTGTCGCAACGTCGCCATTTCGGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCTATCTATCAAACCTTCGCCCA
TCAATCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTATCAGGTATACGCCACTCGTAAAGAAGTTGTGGATAGCCGAGCGGATGCATTAACCAAGGCTGGCTTCAAACCT
GTGGTGGTGGATACACAGGCGCATGGGCTACTTAATATCTGGCAATTGGCCTCGCGCCTGTATCCCGATAAGAAAAACTG
GTTGCTAGTGGATGTTGGAATCGACCAAACCTCGCTAGGGGTTATCCCGCAGAATTCGGCGCCTTTCTATAAAGACATCG
CCTTTGGCACTCAAGATCTTCGCAGCTCAGATGACCCGAGTGATATCGAGAGCGTGTTCGGTACTATGGAAGAGACACAT
CAATTCATTGTTAACCTGATTGAGAAGCTCAAGCGTCAATTGCAGCTCTATTCATCGGGAAATGCGCAGCAACCTATCTC
AGGGATTTGGTTGATGGGCGAGGGCGCCAGCATCCCTATGGTAACCGAAGAGCTAGAACGCCATTTTCAGTTGAGCTGCG
AGTCATTGAATCCTTTGTCTTTATTTGAGAATAAGGTCGCCAAGAGACGCCGACTGCCGATTGACTGGCAACACTTTGGT
ATCGCGGCGGGTATGGCGATGAGTGGGCTCAAGTGGCAGGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-328)

3D structure

• AlphaFold DB: A0A4U2B7T4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	54.839	100	0.548
pilM	Vibrio campbellii strain DS40M4	48.688	100	0.49