Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABQF78_RS18110 Genome accession   NZ_CP168120
Coordinates   4161755..4162888 (+) Length   377 a.a.
NCBI ID   WP_070691750.1    Uniprot ID   -
Organism   Xanthomonas campestris strain WHRI 10255     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4156755..4167888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQF78_RS18085 (ABQF78_018085) - 4156931..4158130 (-) 1200 WP_070691753.1 cystathionine gamma-synthase -
  ABQF78_RS18090 (ABQF78_018090) - 4158224..4159594 (-) 1371 WP_014506550.1 pyridoxal-phosphate dependent enzyme -
  ABQF78_RS18095 (ABQF78_018095) - 4159826..4160044 (-) 219 WP_019237985.1 YdcH family protein -
  ABQF78_RS18100 (ABQF78_018100) - 4160386..4161276 (-) 891 WP_070691751.1 hypothetical protein -
  ABQF78_RS18105 (ABQF78_018105) - 4161273..4161656 (-) 384 WP_040941833.1 DUF4398 domain-containing protein -
  ABQF78_RS18110 (ABQF78_018110) pilU 4161755..4162888 (+) 1134 WP_070691750.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABQF78_RS18115 (ABQF78_018115) maiA 4163410..4164072 (-) 663 WP_070691748.1 maleylacetoacetate isomerase -
  ABQF78_RS18120 (ABQF78_018120) - 4164116..4165105 (-) 990 WP_070691746.1 fumarylacetoacetate hydrolase family protein -
  ABQF78_RS18125 (ABQF78_018125) - 4165485..4166873 (+) 1389 WP_070691744.1 DEAD/DEAH box helicase -
  ABQF78_RS18130 (ABQF78_018130) queD 4166989..4167342 (+) 354 WP_070691742.1 6-carboxytetrahydropterin synthase QueD -
  ABQF78_RS18135 (ABQF78_018135) - 4167532..4167867 (+) 336 WP_070691741.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41839.17 Da        Isoelectric Point: 6.1401

>NTDB_id=1042378 ABQF78_RS18110 WP_070691750.1 4161755..4162888(+) (pilU) [Xanthomonas campestris strain WHRI 10255]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNTMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1042378 ABQF78_RS18110 WP_070691750.1 4161755..4162888(+) (pilU) [Xanthomonas campestris strain WHRI 10255]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGCCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CCGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACAACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTAGGCCTGGACACGCACGCCTTCCAGAG
TGCGCTGAAGAACACGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAAGCGG
CGATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGTGCGCACAAGAACGTGCTGATGAACCTGGCACTGAATCTGCGCGCAGTGGTGTCCCAGCG
CCTGGTCAAGGGCCTGGACGGGCGCCGGCGCCCGGCCACCGAGGTGCTACTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGGCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
ATTCCGTCTTTCCGAAGGCGGCTCCGGCGAGCACGATCCGTATGCCGACTACGAGTCGGCCTCGGCGCCGAAGATCAGCC
ATGGGTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.646

95.225

0.501
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.718

93.899

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.503

100

0.451
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366

Multiple sequence alignment