Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABRP17_RS10845 Genome accession   NZ_CP167826
Coordinates   2479740..2480867 (-) Length   375 a.a.
NCBI ID   WP_188325975.1    Uniprot ID   A0A7H1AN20
Organism   Stenotrophomonas sp. WHRI 8082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2474740..2485867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABRP17_RS10830 (ABRP17_010830) - 2476059..2477048 (+) 990 WP_349986811.1 fumarylacetoacetate hydrolase family protein -
  ABRP17_RS10835 (ABRP17_010835) maiA 2477045..2477731 (+) 687 WP_349986813.1 maleylacetoacetate isomerase -
  ABRP17_RS10840 (ABRP17_010840) glmS 2477810..2479648 (+) 1839 WP_349986815.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -
  ABRP17_RS10845 (ABRP17_010845) pilU 2479740..2480867 (-) 1128 WP_188325975.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABRP17_RS10850 (ABRP17_010850) - 2480994..2481362 (+) 369 WP_192081121.1 DUF4398 domain-containing protein -
  ABRP17_RS10855 (ABRP17_010855) - 2481362..2482246 (+) 885 WP_349984979.1 OmpA family protein -
  ABRP17_RS10860 (ABRP17_010860) - 2482570..2482809 (+) 240 WP_188325978.1 YdcH family protein -
  ABRP17_RS10865 (ABRP17_010865) - 2483009..2484379 (+) 1371 WP_349984980.1 pyridoxal-phosphate dependent enzyme -
  ABRP17_RS10870 (ABRP17_010870) - 2484506..2485696 (+) 1191 WP_349984981.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41517.70 Da        Isoelectric Point: 5.8013

>NTDB_id=1039639 ABRP17_RS10845 WP_188325975.1 2479740..2480867(-) (pilU) [Stenotrophomonas sp. WHRI 8082]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADTGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFNSALKNAMREAPDVILIGEILDSETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHRNVLMNLSLNLRGVISQRLVKGKDGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRMAKDGTIEIEEALRAADSRDGLALKFRLSEGSDGEHDPYADFSEGQPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1039639 ABRP17_RS10845 WP_188325975.1 2479740..2480867(-) (pilU) [Stenotrophomonas sp. WHRI 8082]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCTTCGGACATGTTCTTGACTACCGGTGCGCCGGTCTA
CATCAAGATCGAGGGCAAGCTGTACCCGCTCGGCAACACCGGCCTGCCGCCCGGCATGGTCAAGAAAATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCTCAGTTCGAACGCGAGCTGGAACTCAACATGGCGATTGCCCTGGCGGACACCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGTGGCGAAGTCGGTATGGTCATCCGCGCCATCCGTAGCCGGATACCCAGCATCGA
AGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGCCTGGTCCTGGTGGTGGGGTCCACCGGGT
CGGGCAAGTCCACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACGACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAAATGTCGATCGTCAACCAGCGCGAGGTGGGGCTGGATACGCATGCCTTCAACAG
CGCCTTGAAGAACGCCATGCGTGAAGCACCCGACGTGATCCTGATCGGCGAAATCCTGGATTCGGAAACGATGGAGGCGG
CCATTGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAATGCCGACCAGACGATCGAGCGCATC
CTGAACTTCTTCCCGGAAAGCGCCCACCGCAATGTGCTGATGAACCTGTCGCTGAACCTGCGCGGCGTCATCAGCCAACG
CCTGGTAAAGGGCAAGGACGGCCGCCGCCTGCCCGCAACCGAGGTCCTGATCAACACGCCGATGATCCGAGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGGCCGCGATGGAAGCGTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGCATGGCGAAGGACGGCACCATCGAGATCGAGGAAGCACTGCGCGCCGCCGACTCCCGCGACGGCCTGGCCTTGAA
GTTCCGCCTGTCCGAAGGAAGCGACGGTGAACACGACCCCTACGCCGACTTCTCCGAAGGCCAACCCAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H1AN20

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

95.733

0.493
  pilU Acinetobacter baylyi ADP1

47.861

99.733

0.477
  pilU Vibrio cholerae strain A1552

49.719

94.933

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.251

95.467

0.432
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii strain A118

41.42

90.133

0.373
  pilT Acinetobacter nosocomialis M2

41.42

90.133

0.373
  pilT Acinetobacter baumannii D1279779

41.42

90.133

0.373
  pilT Acinetobacter baylyi ADP1

41.176

90.667

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.828

90.133

0.368
  pilT Legionella pneumophila strain Lp02

40.828

90.133

0.368
  pilT Legionella pneumophila strain ERS1305867

40.828

90.133

0.368

Multiple sequence alignment