Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB9L18_RS17745 Genome accession   NZ_CP167120
Coordinates   3886288..3887415 (+) Length   375 a.a.
NCBI ID   WP_100554661.1    Uniprot ID   A0AAW4GKF4
Organism   Stenotrophomonas lactitubi strain RW9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3881288..3892415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9L18_RS17720 (AB9L18_17720) - 3881390..3882562 (-) 1173 WP_375068137.1 cystathionine gamma-synthase -
  AB9L18_RS17725 (AB9L18_17725) - 3882686..3884056 (-) 1371 WP_346559836.1 pyridoxal-phosphate dependent enzyme -
  AB9L18_RS17730 (AB9L18_17730) - 3884360..3884596 (-) 237 WP_093818802.1 YdcH family protein -
  AB9L18_RS17735 (AB9L18_17735) - 3884920..3885804 (-) 885 WP_375068139.1 hypothetical protein -
  AB9L18_RS17740 (AB9L18_17740) - 3885801..3886166 (-) 366 WP_099820401.1 DUF4398 domain-containing protein -
  AB9L18_RS17745 (AB9L18_17745) pilU 3886288..3887415 (+) 1128 WP_100554661.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB9L18_RS17750 (AB9L18_17750) - 3887547..3888830 (+) 1284 WP_375068142.1 putative DNA modification/repair radical SAM protein -
  AB9L18_RS17755 (AB9L18_17755) - 3888811..3890283 (+) 1473 Protein_3473 UdgX family uracil-DNA binding protein -
  AB9L18_RS17760 (AB9L18_17760) maiA 3890317..3890985 (-) 669 WP_375068144.1 maleylacetoacetate isomerase -
  AB9L18_RS17765 (AB9L18_17765) - 3891000..3891989 (-) 990 WP_180166677.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41388.62 Da        Isoelectric Point: 6.2378

>NTDB_id=1038274 AB9L18_RS17745 WP_100554661.1 3886288..3887415(+) (pilU) [Stenotrophomonas lactitubi strain RW9]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHTFHDALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKNKEGRRLPATEVLINTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKNGTIDQEEALRAADSRDGLALKFRLSEGGSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1038274 AB9L18_RS17745 WP_100554661.1 3886288..3887415(+) (pilU) [Stenotrophomonas lactitubi strain RW9]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAGGGCAAGCTGTATCCGCTCGGCAATACCGGCCTGCCGCCGGGAATGGTGAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCCCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGGCCGATGCCGGGCGT
TTCCGCGTCAACGTGTTCAAGCAGCGCGGTGAAGTGGGCATGGTCATCCGCGCCATCCGCAGCCGGATTCCGAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACTCCGCGCGGGCTGGTGCTGGTGGTGGGGTCCACCGGCT
CGGGCAAGTCCACCTCGCTGGCATCGATGATCGATCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTCGATACCCACACCTTCCACGA
CGCGCTGAAGAACGCGATGCGTGAAGCACCCGACGTGATCCTGATCGGCGAGATCCTGGATGCGGAAACGATGGAGGCGG
CAATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCCACCCTGCATTCCAACAACGCCGATCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGGTCAGCCAGCG
TCTGGTGAAGAACAAGGAGGGGCGCCGCCTGCCAGCCACCGAAGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAGATCAAGGCAGCAATGGAGGCGTCGCTGGAAGAAGGCATGGAGAGCTTCGACCAGTGCCTG
TTCCGTTTGGCCAAGAACGGCACCATCGATCAGGAAGAGGCACTGCGTGCGGCCGACTCCCGCGATGGCCTGGCGCTGAA
ATTCCGCCTGTCCGAAGGCGGCAGCGGCGAGCATGATCCGTATGCGGACTTCTCGGCCGGCGCACCGAGCATTACCCACG
GGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

95.733

0.493
  pilU Vibrio cholerae strain A1552

48.889

96

0.469
  pilU Acinetobacter baylyi ADP1

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.04

99.467

0.448
  pilT Pseudomonas aeruginosa PAK

42.308

90.133

0.381
  pilT Legionella pneumophila strain ERS1305867

41.124

90.133

0.371
  pilT Acinetobacter nosocomialis M2

41.124

90.133

0.371
  pilT Acinetobacter baumannii D1279779

41.124

90.133

0.371
  pilT Acinetobacter baumannii strain A118

41.124

90.133

0.371
  pilT Legionella pneumophila strain Lp02

41.124

90.133

0.371
  pilT Acinetobacter baylyi ADP1

40.882

90.667

0.371
  pilT Pseudomonas stutzeri DSM 10701

40.828

90.133

0.368

Multiple sequence alignment