Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8P72_RS01605 Genome accession   NZ_CP166085
Coordinates   361503..362624 (-) Length   373 a.a.
NCBI ID   WP_068406407.1    Uniprot ID   A0A2I0ELN7
Organism   Psychrobacter sp. CLB018     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356503..367624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8P72_RS01575 (AB8P72_01580) - 357016..357351 (+) 336 WP_068406397.1 cytochrome c5 family protein -
  AB8P72_RS01580 (AB8P72_01585) - 357714..358382 (+) 669 WP_068406399.1 cytochrome c -
  AB8P72_RS01585 (AB8P72_01590) - 358653..359792 (+) 1140 WP_068406401.1 hypothetical protein -
  AB8P72_RS01590 (AB8P72_01595) - 359780..360178 (+) 399 WP_227514961.1 RnfH family protein -
  AB8P72_RS01595 (AB8P72_01600) - 360201..360620 (-) 420 WP_068406454.1 outer membrane protein assembly factor BamE -
  AB8P72_RS01600 (AB8P72_01605) fur 360933..361367 (+) 435 WP_068406404.1 ferric iron uptake transcriptional regulator -
  AB8P72_RS01605 (AB8P72_01610) pilU 361503..362624 (-) 1122 WP_068406407.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8P72_RS01610 (AB8P72_01615) pilT 362794..363849 (-) 1056 WP_068406410.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8P72_RS01615 (AB8P72_01620) - 364111..364875 (+) 765 WP_082894340.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8P72_RS01620 (AB8P72_01625) - 365097..366374 (-) 1278 WP_193030047.1 hemolysin family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41655.94 Da        Isoelectric Point: 7.3354

>NTDB_id=1033242 AB8P72_RS01605 WP_068406407.1 361503..362624(-) (pilU) [Psychrobacter sp. CLB018]
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLTAEQTMRLVKGVMTPSQQKEFDETNECQFAISDQAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYKNGEITYKEAILHADSPNDLRLKIKLSGKNGNVNLDEGADSLSLDIT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1033242 AB8P72_RS01605 WP_068406407.1 361503..362624(-) (pilU) [Psychrobacter sp. CLB018]
ATGGACATTGATAAGCTTTTACAATTAATGATTAATAAAAACGGCTCTGACCTTTTTATTACTGCTGATGTCGCGCCGTC
CATGAAAATCAATGGCAAAATCTTGCCGGTGGGTAAAACGCCGTTAACCGCTGAGCAAACCATGAGATTGGTCAAAGGGG
TGATGACCCCAAGCCAGCAAAAAGAATTTGACGAAACCAACGAGTGTCAATTTGCGATTTCAGATCAAGCGCAGCAAGCG
CGCTTTCGGGTAAGTGCTTTTATTCAGCGCGACATGGCAGGGATGATTTTACGGAAAATCGAAAATAAAATCCCGACCGT
TGAAGAGCTACGCCTGCCCCCAGCGCTAAAAGAGCTGGCCATGAAAAAGCGCGGTATTATTTTGTTGGTTGGGGCTACAG
GTACGGGTAAATCAACTACCCTTGCGGCGATGATTGGCCATCGTAACCAGAATTCACACGGTCATATTATTACCATCGAA
GATCCGATTGAGTTTGTGCATAAGCACCGCGGTTGTATCATTACCCAGCGCGAAGTTGGTATCGACACCCATTCTTTTGA
GGCAGGTCTTAAAAACACCTTGCGCCAAGCGCCTGATGTGATTTTGATTGGTGAGATTCGTAACCGCGAAGTCATGAGCT
ACGCGATTCAATACGCTGAAACGGGGCATTTGGTCTTTGCAACTTTACACGCCAATAACGCCAACCAAGCGATTGACCGT
ATCATTCACTTTTTTGAAACCAGTCGCCATAATCAGTTGTTTATGGATTTATCATTAAACTTGCAAGCTATTATCGCTCA
GCAATTGATTCCAACGCCTGATGGTAAAGGACGCCGCGCCGCTATTGAGATTTTATTAAACTCACAGCTGATCAGCGACT
ACATCCGTAAAGGCGAGGTGCACGAAATCAAAGATGTCATGACGCGCTCACGTGATAGCGGCATGCAAACCTTTGACCAA
GCGCTGTTTGATTTATATAAAAATGGTGAAATCACTTATAAAGAAGCCATTCTGCACGCTGACTCCCCTAACGATTTACG
CTTAAAGATTAAGTTAAGTGGTAAAAATGGCAACGTCAACTTAGATGAAGGCGCCGATAGTTTATCGTTAGATATCACTT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I0ELN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.952

100

0.66
  pilU Acinetobacter baylyi ADP1

66.575

97.051

0.646
  pilU Vibrio cholerae strain A1552

51.762

98.928

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.53

98.123

0.408
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.194

89.812

0.37
  pilT Vibrio cholerae strain A1552

41.194

89.812

0.37
  pilT Acinetobacter nosocomialis M2

39.942

91.957

0.367
  pilT Acinetobacter baumannii D1279779

39.942

91.957

0.367
  pilT Acinetobacter baumannii strain A118

39.942

91.957

0.367
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362
  pilT Legionella pneumophila strain Lp02

41.538

87.131

0.362
  pilT Legionella pneumophila strain ERS1305867

41.538

87.131

0.362

Multiple sequence alignment