Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABWH88_RS08935 Genome accession   NZ_CP159479
Coordinates   1848791..1849912 (-) Length   373 a.a.
NCBI ID   WP_014575888.1    Uniprot ID   E4PL12
Organism   Marinobacter adhaerens strain ac5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1843791..1854912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWH88_RS08920 (ABWH88_08895) polA 1843896..1846628 (+) 2733 WP_216688060.1 DNA polymerase I -
  ABWH88_RS08925 (ABWH88_08900) - 1846862..1847236 (+) 375 WP_008174194.1 hypothetical protein -
  ABWH88_RS08930 (ABWH88_08905) - 1847268..1848551 (-) 1284 WP_014575889.1 OmpP1/FadL family transporter -
  ABWH88_RS08935 (ABWH88_08910) pilU 1848791..1849912 (-) 1122 WP_014575888.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABWH88_RS08940 (ABWH88_08915) pilT 1849955..1850989 (-) 1035 WP_008174189.1 type IV pilus twitching motility protein PilT Machinery gene
  ABWH88_RS08945 (ABWH88_08920) - 1851130..1851834 (+) 705 WP_041644903.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABWH88_RS08950 (ABWH88_08925) proC 1851896..1852729 (+) 834 WP_014575886.1 pyrroline-5-carboxylate reductase -
  ABWH88_RS08955 (ABWH88_08930) - 1852752..1853336 (+) 585 WP_008174183.1 YggT family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41509.66 Da        Isoelectric Point: 6.5755

>NTDB_id=1013751 ABWH88_RS08935 WP_014575888.1 1848791..1849912(-) (pilU) [Marinobacter adhaerens strain ac5]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTREFVYGAMNDKQRAEFEETHECNFAISARGIGR
FRVSAFFQRNLCGMVLRRIEVKIPQIDDLSLPEVIKELAMTKRGLIMFVGATGTGKSTSLAAMLGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHNQIWMDLSLNLKAIVAQQLVPTPDGKGRKAVIEVLINTPLVSDLIRKGEVHRLKELMSKSNESGMQTFDQA
LYHLYAEGSITYEDALAHADSANDLRLMIKLGADAQGADKLSSSVDKLSIQDD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1013751 ABWH88_RS08935 WP_014575888.1 1848791..1849912(-) (pilU) [Marinobacter adhaerens strain ac5]
ATGGAATTCGAAAAACTGTTACGGCTGATGGTGGAGAAGGGCGGTTCGGACCTGTTTATTACAGCGGGTGTGCCGCCAAG
CATGAAGGTGAACGGGAAAGTTTTGCCGGTCACCAAGAACGCGTTGACGCCGGAGCAGACCAGGGAGTTCGTTTACGGCG
CCATGAACGACAAGCAGCGCGCCGAATTCGAGGAAACCCATGAGTGTAACTTCGCTATCAGTGCCCGTGGCATTGGCCGT
TTCCGTGTTTCTGCCTTTTTCCAGCGCAACCTGTGCGGGATGGTGCTGCGTCGGATCGAGGTAAAGATTCCGCAGATCGA
TGATCTGTCGTTGCCGGAGGTAATCAAAGAACTGGCCATGACCAAGCGTGGCCTGATCATGTTCGTGGGCGCTACCGGTA
CCGGTAAGTCTACGTCCCTGGCGGCCATGCTCGGGCATCGTAACCGCAACAGCCGTGGCCACATCATTTCCATCGAAGAC
CCAATCGAATTCGTGCACCAGCATCAGGGTTGTATCGTTACCCAGCGCGAGGTGGGCATCGATACCGAGAGCTTCGAAGT
AGCGCTGAAGAACACCCTGCGCCAGGCACCAGACGTTATTCTGATCGGTGAGGTTCGTACCCGGCAGACCATGGAATATT
CCGTGCAATTCGCCGAGACCGGTCACCTGTGTCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCCAGTTCTTCCCGCCGGAACAGCACAACCAGATCTGGATGGACCTGTCCCTGAACCTCAAGGCGATCGTGGCTCAGCA
GCTGGTGCCAACGCCAGATGGCAAGGGCCGTAAGGCGGTTATCGAGGTGCTGATCAATACCCCGCTGGTATCGGACCTGA
TCCGGAAGGGCGAGGTGCATCGGCTGAAAGAGCTGATGTCCAAATCCAACGAATCCGGTATGCAGACCTTTGATCAGGCG
CTTTATCACCTGTATGCGGAGGGTTCGATTACCTACGAGGATGCCCTGGCCCACGCAGACTCTGCCAACGACCTGCGCCT
GATGATCAAGCTGGGTGCCGACGCCCAGGGAGCCGACAAGCTCTCGTCTTCCGTCGACAAGCTTTCTATTCAAGATGACT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4PL12

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

79.245

99.464

0.788
  pilU Acinetobacter baylyi ADP1

64.888

95.442

0.619
  pilU Vibrio cholerae strain A1552

57.027

99.196

0.566
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

90.885

0.386
  pilT Pseudomonas aeruginosa PAK

40.896

89.812

0.367
  pilT Acinetobacter baumannii strain A118

40.597

89.812

0.365
  pilT Acinetobacter baumannii D1279779

40.597

89.812

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.597

89.812

0.365
  pilT Acinetobacter nosocomialis M2

40.299

89.812

0.362
  pilT Legionella pneumophila strain Lp02

40.299

89.812

0.362
  pilT Legionella pneumophila strain ERS1305867

40.299

89.812

0.362

Multiple sequence alignment